Protein Overview: BUD7

General Information:
Name(s) found:	BUD7 / YOR299W [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane [SGD]
Organism:	Saccharomyces cerevisiae
Length:	746 amino acids
Gene Ontology:
Cellular Component:	trans-Golgi network transport vesicle [IDA] clathrin-coated vesicle [IDA]
Biological Process:	cellular bud site selection [IMP] Golgi to plasma membrane transport [IGI] cell wall chitin biosynthetic process [IGI][IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MITQNSIPEV KEDFIGYALH ERRIRLPQFQ DLGPADLVTL TKYLPTSSNT NAINSTSRNG  60
   61 AAIIQSPAAV VADDSAASMA TNGDASDTAV TTNYTNASIY SSSRNANDGA PMVAELHPLD 120
  121 KLKDEVGTFF YSMGVDTSGP TSIAIFLKEI SEVISEKPQV WFGRKKTFNV ARISFSTWNA 180
  181 FRRCDINVVV HIPGSIQNFI VDCNGESQNI EMCADYDLIW AETFVSGVVR SIMLMKENAE 240
  241 EGELQNLVET LILNPFTAGQ IDDVPEMFID LFPIVYHKGP LLGAPYYITN VTNTNNYLVE 300
  301 TLVEIVKLTR NVSRAEIMLK NLATDNPEAI IILIKIFLVC DQELDAIKLT YDMLSQDKII 360
  361 NNTNNRMDYK SELLCLQAQF LIDKRQDYSL AQNIAQEAVN CSPSEFRPWY LLSKVYVKLN 420
  421 DIENALLILN SCPMSPLKEK YVLKRVAPLP SNNSLHLPLP IDVVLDEVTS LNPQDVQNEH 480
  481 RSADPMLVNL AASNLKSTFQ LAYRLLTEIV QITGWENLLK YRSNIFVMEE EYQKSSSSLP 540
  541 KDVNKQEEQP LRAKRLCERW LDNLFMLLYE DLKMYTLWQT EQLYMDAQNN NHNKLTFEWE 600
  601 LFGLCARRLG HFPEAAKAFQ NGLSQRFSSR CARKLLEYCI NERQRVKNFI NSPNSHDMVP 660
  661 EIVSSRIREL DNSIIDLCVK ICCWNHRWYT EFSISLLDCL SVVIQDMSLT KVSNEISSRY 720
  721 PETVLNLVQE NLLNFFTTCT IGCYDA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BUD7 RAD17 TDH2 ULA1
View Details	Gavin AC, et al. (2006)		BCH1 BUD7 CHS5 CHS6
View Details	Ho Y, et al. (2002)		BUD7 COR1 GAL7 PRE3 QCR2 SLT2 SMK1 STI1
View Details	Qiu et al. (2008)		BCH2 BUD7 CHS6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUD7	VPS45	2	Unpublished Fields Lab Data
View Screen	BUD7	STE20	2	Unpublished Fields Lab Data
View Screen	BUD7	RRP14	2	Unpublished Fields Lab Data
View Screen	BUD7	HER2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUD7	Huh WK, et al. (2003)
View Data	BUD7 and CHC1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..363]	deduced	N/A	No confident structure predictions are available.
2	View Details	[364..472]	PSI-BLAST	2.221849	Designed protein cTPR2
3	View Details	[473..746]	MSA	1.008926	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2