Protein Overview: GCV2

General Information:
Name(s) found:	GCV2 / YMR189W [SGD]
Description(s) found: Found 31 descriptions. SHOW ALL	P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1034 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ][TAS] glycine cleavage complex [TAS]
Biological Process:	glycine catabolic process [IC] one-carbon metabolic process [IGI][IMP]
Molecular Function:	glycine dehydrogenase (decarboxylating) activity [IEP][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRTRVTALL CRATVRSSTN YVSLARTRSF HSQSILLKTA ATDITSTQYS RIFNPDLKNI  60
   61 DRPLDTFARR HLGPSPSDVK KMLKTMGYSD LNAFIEELVP PNILKRRPLK LEAPSKGFCE 120
  121 QEMLQHLEKI ANKNHYKVKN FIGKGYYGTI LPPVIQRNLL ESPEWYTSYT PYQPEISQGR 180
  181 LEALLNFQTV VSDLTGLPVA NASLLDEGTA AGEAMLLSFN ISRKKKLKYV IDKKLHQQTK 240
  241 SVLHTRAKPF NIEIIEVDCS DIKKAVDVLK NPDVSGCLVQ YPATDGSILP PDSMKQLSDA 300
  301 LHSHKSLLSV ASDLMALTLL KPPAHYGADI VLGSSQRFGV PMGYGGPHAA FFAVIDKLNR 360
  361 KIPGRIVGIS KDRLGKTALR LALQTREQHI KRDKATSNIC TAQALLANVA SSYCVYHGPK 420
  421 GLQNISRRIF SLTSILANAI ENDSCPHELI NKTWFDTLTI KLGNGISSEQ LLDKALKEFN 480
  481 INLFAVDTTT ISLALDETTT KADVENLLKV FDIENSSQFL SEDYSNSFPR EFQRTDEILR 540
  541 NEVFHMHHSE TAMLRYLHRL QSRDLSLANS MIPLGSCTMK LNSTVEMMPI TWPQFSNIHP 600
  601 FQPSNQVQGY KELITSLEKD LCSITGFDGI SLQPNSGAQG EYTGLRVIRS YLESKGENHR 660
  661 NVCLIPVSAH GTNPASAAMA GLKVVPVNCL QDGSLDLVDL KNKAEQHSKE LAAVMITYPS 720
  721 TYGLFEPGIQ HAIDIVHSFG GQVYLDGANM NAQVGLTSPG DLGADVCHLN LHKTFSIPHG 780
  781 GGGPAGAPIC VKSHLIPHLP KHDVVDMITG IGGSKSIDSV SSAPYGNALV LPISYAYIKM 840
  841 MGNEGLPFSS VIAMLNSNYM MTRLKDHYKI LFVNEMSTLK HCAHEFIVDL REYKAKGVEA 900
  901 IDVAKRLQDY GFHAPTLAFP VPGTLMIEPT ESENLEELDR FCDAMISIKE EINALVAGQP 960
  961 KGQILKNAPH SLEDLITSSN WDTRGYTREE AAYPLPFLRY NKFWPTVARL DDTYGDMNLI1020
 1021 CTCPSVEEIA NETE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		GCV1 GCV2 GCV3 LPD1
View Details	Qiu et al. (2008)		GCV2 LPD1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	GCV2	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..111]	deduced	N/A	No confident structure predictions are available.
2	View Details	[112..146] [426..539]	PSI-BLAST	65.522879	NifS-like protein/selenocysteine lyase
3	View Details	[147..425]	PSI-BLAST	65.522879	NifS-like protein/selenocysteine lyase
4	View Details	[540..850] [882..894]	PSI-BLAST	63.0	Cystathionine gamma-synthase, CGS
5	View Details	[851..881] [895..1034]	PSI-BLAST	63.0	Cystathionine gamma-synthase, CGS

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
glycine dehydrogenase (decarboxylating) activity	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	3.14486270422678	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transferase activity	1.44093490824786	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
methyltransferase activity	0.681623261233707	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	0.619695949258279	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.60304280865376	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009

2

Term	Confidence	Notes
glycine dehydrogenase (decarboxylating) activity	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	3.14486270422678	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transferase activity	1.44093490824786	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
methyltransferase activity	0.681623261233707	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	0.619695949258279	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.60304280865376	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009

3

No functions predicted.

4

No functions predicted.

5

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

Philius confidence legend

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle