YRC Public Data Repository - Protein Structure Prediction

Protein:	GSPE_ECOLI
Organism:	Escherichia coli
Length:	493 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GSPE_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|27364324, gi\|... - gi\|27364324\|ref\|NP_759852.1\| Type II secretory pathway, ATPase EpsE [Vibrio vulnificus CMCP6], gi\|27...	487.0	[0..5]	[487..12]
gi\|39983472, gi\|... - gi\|39996591\|ref\|NP_952542.1\| type IV pilus biogenesis protein PilB [Geobacter sulfurreducens PCA], g...	486.0	[0..1]	[489..74]
gi\|37197138, gi\|... - gi\|37678399\|ref\|NP_933008.1\| type II secretory pathway, ATPase EpsE [Vibrio vulnificus YJ016], gi\|37...	486.0	[0..5]	[487..12]
gi\|90437782, gi\|... - gi\|90581086\|ref\|ZP_01236886.1\| putative general secretion pathway protein E [Vibrio angustum S14], g...	486.0	[0..6]	[487..15]
gi\|89049776, gi\|... - gi\|89074488\|ref\|ZP_01160965.1\| putative general secretion pathway protein E [Photobacterium sp. SKA3...	485.0	[0..6]	[487..15]
gi\|85773561, gi\|... - gi\|86157050\|ref\|YP_463835.1\| type II secretion system protein E [Anaeromyxobacter dehalogenans 2CP-C...	484.0	[0..3]	[485..73]
gi\|59713080, gi\|... - gi\|59713080\|ref\|YP_205856.1\| general secretion pathway protein E [Vibrio fischeri ES114], gi\|5948118...	483.0	[0..2]	[487..10]
gi\|48846577, gi\|... - gi\|78222605\|ref\|YP_384352.1\| type II secretion system protein E [Geobacter metallireducens GS-15], g...	483.0	[0..2]	[489..75]
gi\|254222241, gi... - gi\|75824765\|ref\|ZP_00754211.1\| COG2804: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pa... tr\|D7HG13\|D7HG13... - General secretion pathway protein E OS=Vibrio cholerae RC385 GN=VCRC385_01803 PE=4 SV=1	482.0	[0..2]	[487..12]
gi\|153828327, gi... - gi\|153828327\|ref\|ZP_01980994.1\| general secretion pathway protein E [Vibrio cholerae 623-39], gi\|148... gi\|153825524, gi... - gi\|153825524\|ref\|ZP_01978191.1\| general secretion pathway protein E [Vibrio cholerae MZO-2], gi\|1497... gi\|153817431, gi... - gi\|153817431\|ref\|ZP_01970098.1\| general secretion pathway protein E [Vibrio cholerae NCTC 8457], gi\|... gi\|229509859, gi... - gi\|229509859\|ref\|ZP_04399340.1\| general secretion pathway protein E [Vibrio cholerae B33], gi\|229353... gi\|121630855, gi... - gi\|75815847\|ref\|ZP_00746337.1\| COG2804: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pa... gi\|125619251, gi... - gi\|75830657\|ref\|ZP_00759931.1\| COG2804: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pa... GSPE_VIBCH - Type II secretion system protein E OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba ... gi\|194537470, gi... - gi\|75820632\|ref\|ZP_00750675.1\| COG2804: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pa... gi\|227119168, gi... - gi\|75827975\|ref\|ZP_00757410.1\| COG2804: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pa... gi\|227082846, gi... - gi\|227082846\|ref\|YP_002811397.1\| general secretion pathway protein E [Vibrio cholerae M66-2], gi\|227... gi\|229507220, gi... - gi\|229507220\|ref\|ZP_04396725.1\| general secretion pathway protein E [Vibrio cholerae BX 330286], gi\|... gi\|229349537, gi... - gi\|229513660\|ref\|ZP_04403124.1\| general secretion pathway protein E [Vibrio cholerae TMA 21], gi\|229... gi\|229516980, gi... - gi\|229516980\|ref\|ZP_04406426.1\| general secretion pathway protein E [Vibrio cholerae RC9], gi\|229346... gi\|229334974, gi... - gi\|229527342\|ref\|ZP_04416734.1\| general secretion pathway protein E [Vibrio cholerae 12129(1)], gi\|2... gi\|229369381, gi... - gi\|229606726\|ref\|YP_002877374.1\| general secretion pathway protein E [Vibrio cholerae MJ-1236], gi\|2... GSPE_VIBCH - General secretion pathway protein E OS=Vibrio cholerae GN=epsE PE=1 SV=1	482.0	[0..2]	[487..12]

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Predicted Domain #1

Region A:
Residues: [1-86]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM TSLSQADFEA  60
   61 RLEAVFHQNT GESQQIAQDI DQSVDL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	4.69897
Match:	2bh1X
Description:	X-ray structure of the general secretion pathway complex of the N- terminal domain of EpsE and the cytosolic domain of EpsL of Vibrio cholerae
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [87-493]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LSLSEEMPAN EDLLNEDSAA PVIRLINAIL SEAIKETASD IHIETYEKTM SIRFRIDGVL  60
   61 RTILQPNKKL AALLISRIKV MARLDIAEKR IPQDGRISLR IGRRNIDVRV STLPSIYGER 120
  121 AVLRLLDKNS LQLSLNNLGM TAADKQDLEN LIQLPHGIIL VTGPTGSGKS TTLYAILSAL 180
  181 NTPGRNILTV EDPVEYELEG IGQTQVNTRV DMSFARGLRA ILRQDPDVVM VGEIRDTETA 240
  241 QIAVQASLTG HLVLSTLHTN SASGAVTRLR DMGVESFLLS SSLAGIIAQR LVRRLCPQCR 300
  301 QFTPVSPQQA QMFKYHQLAV TTIGTPVGCP HCHQSGYQGR MAIHEMMVVT PELRAAIHEN 360
  361 VDEQALERLV RQQHKALIKN GLQKVISGDT SWDEVMRVAS ATLESEA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	124.0
Match:	1p9rA
Description:	Crystal Structure of Vibrio cholerae putative NTPase EpsE
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
protein transporter activity	4.96050219760327	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
transporter activity	2.58140740499725	bayes_pls_golite062009
transmembrane transporter activity	2.39695871109024	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
ATPase activity, coupled	2.18396165590908	bayes_pls_golite062009
primary active transmembrane transporter activity	1.80249404515499	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	1.79104521657582	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	1.72596995524566	bayes_pls_golite062009
active transmembrane transporter activity	1.72417939639501	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	1.71919653053162	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	1.71868752128533	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
binding	1.55526868300981	bayes_pls_golite062009
substrate-specific transporter activity	1.29730375126024	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.906554174337376	bayes_pls_golite062009
motor activity	0.87979065815418	bayes_pls_golite062009
protein binding	0.569246647740442	bayes_pls_golite062009
catalytic activity	0.458437698837681	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
guanyl nucleotide binding	0.373350189511011	bayes_pls_golite062009
guanyl ribonucleotide binding	0.363151049799385	bayes_pls_golite062009
ion transmembrane transporter activity	0.355466224802969	bayes_pls_golite062009
GTP binding	0.343113158179248	bayes_pls_golite062009
GTPase activity	0.105546189348878	bayes_pls_golite062009
cation transmembrane transporter activity	0.0254369227200564	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: