YRC Public Data Repository - Protein Structure Prediction

Protein:	LEU2
Organism:	Saccharomyces cerevisiae
Length:	364 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for LEU2.

Description	E-value	Query Range	Subject Range
LEU3_YEAST - 3-isopropylmalate dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LEU2 PE=... LEU2 - Beta-isopropylmalate dehydrogenase (IMDH), catalyzes the third step in the leucine biosynthesis path... gi\|223364527 - gi\|223364527\|gb\|ACM86781.1\| beta-isopropylmalate dehydrogenase [Cloning vector pEG(KG)] gi\|156079882 - gi\|156079882\|gb\|ABU48524.1\| beta-isopropyl-malate dehydrogenase [Cloning vector pAW8-yEGFP] gi\|156079872 - gi\|156079872\|gb\|ABU48518.1\| beta-isopropyl-malate dehydrogenase [Cloning vector pAW8] gi\|23506934 - gi\|23506934\|gb\|AAN31951.1\| 3-isopropylmalate dehydrogenase; Leu2p [Cloning vector YDp-L] gi\|156079869 - gi\|156079869\|gb\|ABU48516.1\| beta-isopropyl-malate dehydrogenase [Cloning vector pAW5] gi\|207347360 - gi\|207347360\|gb\|EDZ73556.1\| YCL018Wp-like protein [Saccharomyces cerevisiae AWRI1631] gi\|209167984 - gi\|209167984\|gb\|ACI42001.1\| beta-isopropyl-malate dehydrogenase [Cre-expression plasmid pAW8_Xho] gi\|223364522 - gi\|223364522\|gb\|ACM86777.1\| beta-isopropylmalate dehydrogenase [Cloning vector pEG(KT)]	0.0	[1..364]	[1..364]
gi\|2692340 - gi\|2692340\|gb\|AAB91428.1\| beta-isopropylmalate dehydrogenase [Expression vector pESP-2]	0.0	[1..364]	[1..364]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[1..364]	[1..364]
gi\|6318865 - gi\|6318865\|gb\|AAF07053.1\|AF178583_3 dLEU2 [Expression vector pSB229] gi\|5929926, gi\|5... - gi\|5929926\|gb\|AAD56650.1\|AF178973_5 LEU2 [Cloning vector pCS512], gi\|5929923\|gb\|AAD56647.1\|AF178973_... gi\|6318857 - gi\|6318857\|gb\|AAF07046.1\|AF178582_1 LEU2p [Expression vector pCS316] gi\|6457299 - gi\|6457299\|gb\|AAF09462.1\|AF179626_3 dLEU2 [Expression vector pGP100] gi\|6457310 - gi\|6457310\|gb\|AAF09471.1\|AF179628_4 dLEU2 [Shuttle vector pHIGEXhOR] gi\|6457291, gi\|6... - gi\|6457295\|gb\|AAF09459.1\|AF179625_5 LEU2 [Shuttle vector pCS513], gi\|6457291\|gb\|AAF09455.1\|AF179625_...	0.0	[1..364]	[5..368]
gi\|1595849 - gi\|1595849\|gb\|AAB49965.1\| LEU2 [Cloning vector pGAD-C3] gi\|1595845 - gi\|1595845\|gb\|AAB49962.1\| LEU2 [Cloning vector pGAD-C2] gi\|1595841 - gi\|1595841\|gb\|AAB49959.1\| LEU2 [Cloning vector pGAD-C1]	0.0	[1..364]	[1..364]
gi\|15289746 - gi\|15289746\|dbj\|BAB63465.1\| b-isopropylmalate dehydrogenase [Saccharomyces naganishii] LEU3_SACEX - 3-isopropylmalate dehydrogenase OS=Saccharomyces exiguus GN=LEU2 PE=3 SV=1	0.0	[3..364]	[4..365]
gi\|13569825 - gi\|13569825\|gb\|AAK31262.1\| LEU2 marker [Integrating expression vector pARC25B]	0.0	[1..364]	[5..368]
gi\|11344907 - gi\|11344907\|gb\|AAG34541.1\| beta-isopropylmalate dehydrogenase [PCR template vector pJJH727] LEU3_KLULA - 3-isopropylmalate dehydrogenase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / N... LEU3_KLULA - 3-isopropylmalate dehydrogenase OS=Kluyveromyces lactis GN=LEU2 PE=3 SV=2	0.0	[5..364]	[3..362]
gi\|1160359 - gi\|1160359\|gb\|AAC53643.1\| 3-isopropylmalate dehydrogenase	0.0	[1..364]	[1..366]

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Predicted Domain #1

Region A:
Residues: [1-364]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAPKKIVVL PGDHVGQEIT AEAIKVLKAI SDVRSNVKFD FENHLIGGAA IDATGVPLPD  60
   61 EALEASKKAD AVLLGAVGGP KWGTGSVRPE QGLLKIRKEL QLYANLRPCN FASDSLLDLS 120
  121 PIKPQFAKGT DFVVVRELVG GIYFGKRKED DGDGVAWDSE QYTVPEVQRI TRMAAFMALQ 180
  181 HEPPLPIWSL DKANVLASSR LWRKTVEETI KNEFPTLKVQ HQLIDSAAMI LVKNPTHLNG 240
  241 IIITSNMFGD IISDEASVIP GSLGLLPSAS LASLPDKNTA FGLYEPCHGS APDLPKNKVN 300
  301 PIATILSAAM MLKLSLNLPE EGKAIEDAVK KVLDAGIRTG DLGGSNSTTE VGDAVAEEVK 360
  361 KILA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	954.54902
Match:	2ayqA_
Description:	3-isopropylmalate dehydrogenase, IPMDH
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
isocitrate dehydrogenase (NAD+) activity	5.9567768649084	bayes_pls_golite062009
isocitrate dehydrogenase activity	5.76817846591752	bayes_pls_golite062009
3-isopropylmalate dehydrogenase activity	4.43187390953534	bayes_pls_golite062009
isocitrate dehydrogenase (NADP+) activity	3.47645456015586	bayes_pls_golite062009
malate dehydrogenase activity	3.17013707624362	bayes_pls_golite062009
oxidoreductase activity	2.74237430402883	bayes_pls_golite062009
catalytic activity	2.71251959637136	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	2.46799258909805	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	2.38235863668553	bayes_pls_golite062009
binding	0.768867938429375	bayes_pls_golite062009
malic enzyme activity	0.483776792839856	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.466038297142601	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: