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Protein Overview: ISOA1_ARATH

General Information:
Name(s) found: ISOA1_ARATH [Swiss-Prot]
Description(s) found:
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  • gi|75096955|sp|O04196.1|ISOA1_ARATH RecName: Full=Isoamylase 1, chloroplastic; Short=AtISA1; Flags: Precursor [NCBI NR]
  • gi|34098817|gb|AAQ56791.1| At2g39930 [Arabidopsis thaliana] [NCBI NR]
  • pir||B84823 probable isoamylase [imported] - Arabidopsis thaliana [NCBI NR]
  • gi|23306386|gb|AAN17420.1| putative isoamylase [Arabidopsis thaliana] [NCBI NR]
  • gi|2088646|gb|AAB95278.1| putative isoamylase [Arabidopsis thaliana] [NCBI NR]
  • gi|15225595|ref|NP_181522.1| ISA1 (ISOAMYLASE 1); alpha-amylase/ isoamylase [Arabidopsis thaliana] [NCBI NR]
  • Isoamylase 1, chloroplastic OS=Arabidopsis thaliana GN=ISA1 PE=1 SV=1 [Swiss-Prot]
  • (O04196) Putative isoamylase [athaliana3702.FASTAC.txt]
  • At2g39930 [Arabidopsis thaliana]gi|23306386|gb|AAN17420.1| putative isoamylase [Arabidopsis thaliana]gi|2088646|gb|AAB95278.1| putative isoamylase [Arabidopsis thaliana]gi|15225595|ref|NP_181522.1| isoamylase, putative / starch debranching enzyme, puta [nrdb_11152004_Chlamydomonas]
  • Tax_Id=3702 Encodes an isoamylase-type debranching enzyme [EBI-IPI_Arabidopsis_3.11_12-06-2005_reversed.fasta]
  • | Symbols: ATISA1, ISA1 | ATISA1/ISA1 (ISOAMYLASE 1); alpha-amylase/ isoamylase | chr2:16673156-16679261 FORWARD [TAIR_Arabidopsis_na_04-12-2008_con_reversed.fasta]
  • At2g39930 [Arabidopsis thaliana] [nr-271106-contam.fasta]
  • putative isoamylase [Arabidopsis thaliana] [nr-271106-contam.fasta]
  • ATISA1/ISA1; alpha-amylase/ isoamylase [Arabidopsis thaliana] [nr-271106-contam.fasta]
  • | Symbols: ISA1, ATISA1 | isoamylase 1 | chr2:16666078-16672183 FORWARD LENGTH=783 [TAIR_A_Thaliana_10_12-14-2010_reversed.fasta]
    • Organism: Arabidopsis thaliana
    Length: 783 amino acids

    Gene Ontology:
    Cellular Component: chloroplast [IDA]
    chloroplast isoamylase complex [IDA]
    Biological Process: carbohydrate metabolic process [ISS]
    amylopectin biosynthetic process [IMP]
    Molecular Function: alpha-amylase activity [ISS]
    isoamylase activity [IDA][IMP]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDAIKCSSSF LHHTKLNTLF SNHTFPKISA PNFKPLFRPI SISAKDRRSN EAENIAVVEK  60
   61 PLKSDRFFIS DGLPSPFGPT VRDDGVNFSV YSTNSVSATI CLISLSDLRQ NKVTEEIQLD 120
  121 PSRNRTGHVW HVFLRGDFKD MLYGYRFDGK FSPEEGHYYD SSNILLDPYA KAIISRDEFG 180
  181 VLGPDDNCWP QMACMVPTRE EEFDWEGDMH LKLPQKDLVI YEMHVRGFTR HESSKIEFPG 240
  241 TYQGVAEKLD HLKELGINCI ELMPCHEFNE LEYYSYNTIL GDHRVNFWGY STIGFFSPMI 300
  301 RYASASSNNF AGRAINEFKI LVKEAHKRGI EVIMDVVLNH TAEGNEKGPI FSFRGVDNSV 360
  361 YYMLAPKGEF YNYSGCGNTF NCNHPVVRQF ILDCLRYWVT EMHVDGFRFD LGSIMSRSSS 420
  421 LWDAANVYGA DVEGDLLTTG TPISCPPVID MISNDPILRG VKLIAEAWDA GGLYQVGMFP 480
  481 HWGIWSEWNG KFRDVVRQFI KGTDGFSGAF AECLCGSPNL YQGGRKPWHS INFICAHDGF 540
  541 TLADLVTYNN KNNLANGEEN NDGENHNYSW NCGEEGDFAS ISVKRLRKRQ MRNFFVSLMV 600
  601 SQGVPMIYMG DEYGHTKGGN NNTYCHDNYM NYFRWDKKEE AHSDFFRFCR ILIKFRDECE 660
  661 SLGLNDFPTA KRLQWHGLAP EIPNWSETSR FVAFSLVDSV KKEIYVAFNT SHLATLVSLP 720
  721 NRPGYRWEPF VDTSKPSPYD CITPDLPERE TAMKQYRHFL DANVYPMLSY SSIILLLSPI 780
  781 KDP

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..783] PSI-BLAST 93.0 STRUCTURE OF PSEUDOMONAS ISOAMYLASE

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • hydrolase activity, acting on glycosyl bonds
    		•  3.1190602686215 bayes_pls_golite062009
  • hydrolase activity, hydrolyzing O-glycosyl compounds
    		•  2.98773241907716 bayes_pls_golite062009
  • hydrolase activity
    		•  2.87134532188836 bayes_pls_golite062009
  • catalytic activity
    		•  2.22613067995402 bayes_pls_golite062009
  • glycogen debranching enzyme activity
    		•  1.61342226744611 bayes_pls_golite062009
  • 1,4-alpha-glucan branching enzyme activity
    		•  0.72343541139639 bayes_pls_golite062009
  • chitinase activity
    		•  0.67854733864231 bayes_pls_golite062009
  • glucosidase activity
    		•  0.58717079348538 bayes_pls_golite062009
  • hexosaminidase activity
    		•  0.16438321606077 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  0.15340916113777 bayes_pls_golite062009
  • amylase activity
    		•  0.0914002140078796 bayes_pls_golite062009
  • alpha-amylase activity
    		•  0.0521244165288097 bayes_pls_golite062009


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.94

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle