Protein Overview: DFR1

General Information:
Name(s) found:	DFR1 / YOR236W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|68844666\|sp\|P07807.2\|DYR_YEAST RecName: Full=Dihydrofolate reductase [NCBI NR] pir\|\|RDBYD dihydrofolate reductase (EC 1.5.1.3) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6324810\|ref\|NP_014879.1\| Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function; Dfr1p [Saccharomyces cerevisiae] [NCBI NR] gi\|295604\|gb\|AAA34564.1\| dihydrofolate reductase [NCBI NR] gi\|171397\|gb\|AAB59331.1\| dihydrofolate reductase [Saccharomyces cerevisiae] [NCBI NR] gi\|1420541\|emb\|CAA99456.1\| DFR1 [Saccharomyces cerevisiae] [NCBI NR] Dihydrofolate reductase OS=Saccharomyces cerevisiae GN=DFR1 PE=1 SV=2 [Swiss-Prot] DFR1 Dihydrofolate reductase [mips] Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function [SGD] Chr XV from 780906-781541 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 780905-781540 [yeastorf3.fasta] DFR1, Chr XV from 780905-781540 [yeast_orfs2] DFR1 SGDID:S0005762, Chr XV from 780906-781541, Verified ORF [yeastflysequest.fasta] Dfr1p [Saccharomyces cerevisiae]gi\|1420541\|emb\|CAA99456.1\| DFR1 [Saccharomyces cerevisiae]gi\|66097\|pir\|\|RDBYD dihydrofolate reductase (EC 1.5.1.3) - yeast (Saccharomyces cerevisiae)gi\|171397\|gb\|AAB59331.1\| dihydrofolate reductasegi\|295604\|gb\|AAA34564. [nrdb_11152004_Chlamydomonas] DFR1 SGDID:S000005762, Chr XV from 780906-781541, Verified ORF, "Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DFR1 SGDID:S000005762, Chr XV from 780907-781542, Verified ORF, "Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function" [yeast-contam.fasta] dihydrofolate reductase [nr-271106-contam.fasta] Dihydrofolate reductase [nr-271106-contam.fasta] DFR1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function; Dfr1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Dihydrofolate reductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DFR1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DFR1 SGDID:S000005762, Chr XV from 780906-781541, Genome Release 64-1-1, Verified ORF, "Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function" [yeast-030211-contam.fasta] DFR1 SGDID:S000005762, Chr XV from 780906-781541, Genome Release 64-2-1, Verified ORF, "Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism" [20141208_orf_trans.fasta] YOR236W SGDID:S000005762, Chr XV from 780906-781541, Genome Release 64-1-1, Verified ORF, "Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	211 amino acids
Gene Ontology:
Cellular Component:	cytosol [TAS]
Biological Process:	folic acid and derivative metabolic process [IMP]
Molecular Function:	dihydrofolate reductase activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK KNALIMGRKT  60
   61 WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS LANAIMNLES NFKEHLERIY 120
  121 VIGGGEVYSQ IFSITDHWLI TKINPLDKNA TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP 180
  181 PKVELPETDC DQRYSLEEKG YCFEFTLYNR K

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CAP1 DFR1 INP54

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PUF3	DFR1	1	Unpublished Fields Lab Data
View Screen	YOR203W	DFR1	1	Unpublished Fields Lab Data
View Screen	YHR122W	DFR1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DFR1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..211]	PSI-BLAST	389.616428	Dihydrofolate reductases, eukaryotic type

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
dihydrofolic acid binding	5.17709559620199	bayes_pls_golite062009
dihydrofolate reductase activity	4.66343252900911	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4.14137539326183	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH group of donors	3.88025481634404	bayes_pls_golite062009
catalytic activity	1.48020895637259	bayes_pls_golite062009
oxidoreductase activity	1.39855499164233	bayes_pls_golite062009
binding	0.576814191883416	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.72

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2