Protein Overview: IME4

General Information:
Name(s) found:	IME4 / YGL192W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|945046\|gb\|AAA74443.1\| putative mRNA methyltransferase Ime4p [Saccharomyces cerevisiae] [NCBI NR] gi\|6321246\|ref\|NP_011323.1\| Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells; Ime4p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48509 transcription regulator SPO8 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|471168\|dbj\|BAA04938.1\| positive transcriptional regulator for IME2 [Saccharomyces cerevisiae] [NCBI NR] gi\|1322815\|emb\|CAA96904.1\| IME4 [Saccharomyces cerevisiae] [NCBI NR] gi\|1177636\|emb\|CAA62952.1\| transcriptional activator SPO8 [Saccharomyces cerevisiae] [NCBI NR] gi\|1174426\|sp\|P41833.1\|IME4_YEAST RecName: Full=N6-adenosine-methyltransferase IME4 [NCBI NR] N6-adenosine-methyltransferase IME4 OS=Saccharomyces cerevisiae GN=IME4 PE=1 SV=1 [Swiss-Prot] IME4 Positive transcription factor for IME2 [mips] Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids [SGD] IME4, Chr VII from 142250-144052 [yeast_orfs2] Chr VII from 142250-144052 [yeastorf3.fasta] (P41833) N6-adenosine-methyltransferase IME4 (EC 2.1.1.62) [4932.FASTACCERV.txt] IME4 SGDID:S0003160, Chr VII from 142250-144052, Verified ORF [yeastflysequest.fasta] IME4 SGDID:S0003160, Chr VII from 142252-144054, Verified ORF [10112005cervSGD.fasta] Ime4p [Saccharomyces cerevisiae]gi\|945046\|gb\|AAA74443.1\| putative mRNA methyltransferase Ime4p [Saccharomyces cerevisiae]gi\|1322815\|emb\|CAA96904.1\| IME4 [Saccharomyces cerevisiae]gi\|1177636\|emb\|CAA62952.1\| transcriptional activator SPO8 [Saccharomyces [nrdb_11152004_Chlamydomonas] IME4 SGDID:S000003160, Chr VII from 142252-144054, Verified ORF, "Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] IME4 SGDID:S000003160, Chr VII from 142252-144054, Verified ORF, "Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; [yeast-200209-contam.fasta] N6-adenosine-methyltransferase IME4 [nr-271106-contam.fasta] putative mRNA methyltransferase Ime4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells; Ime4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] transcriptional activator SPO8 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] IME4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] positive transcriptional regulator for IME2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] IME4 SGDID:S000003160, Chr VII from 142252-144054, Verified ORF, "Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids" [yeast-bovrabprots.fasta] [chemostatdb.fasta] N6-adenosine-methyltransferase IME4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IME4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] IME4 SGDID:S000003160, Chr VII from 142246-144048, Genome Release 64-1-1, Verified ORF, "Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids" [yeast-030211-contam.fasta] IME4 SGDID:S000003160, Chr VII from 142246-144048, Genome Release 64-2-1, Verified ORF, "mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70)" [20141208_orf_trans.fasta] YGL192W SGDID:S000003160, Chr VII from 142246-144048, Genome Release 64-1-1, Verified ORF, "Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	600 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	meiosis [IMP] mRNA modification [IMP][ISS]
Molecular Function:	mRNA methyltransferase activity [IDA][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD EVLQFEQFWN  60
   61 DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY NKFHAFYYTL KSSSSSCEEK 120
  121 VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK 180
  181 ERAKYTTEVI EYIPICSDYS HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS 240
  241 KIHFIPNIKP QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 300
  301 SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA WNIHMNLPYG 360
  361 TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW GYNVINEVSW IKTNQLGRTI 420
  421 VTGRTGHWLN HSKEHLLVGL KGNPKWINKH IDVDLIVSMT RETSRKPDEL YGIAERLAGT 480
  481 HARKLEIFGR DHNTRPGWFT IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK 540
  541 QPSKLQQQHQ QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ 600
  601

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		CAT5 IME4 PRP28

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUR2	IME4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..295]	deduced	N/A	No confident structure predictions are available.
2	View Details	[296..532]	MSA	6.024967	View MSA. No confident structure predictions are available.
3	View Details	[533..600]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
methyltransferase activity	3.68431317301075	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	3.60853685152199	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	2.91374296950366	bayes_pls_golite062009
DNA-methyltransferase activity	2.72106897862429	bayes_pls_golite062009
RNA methyltransferase activity	2.63941856307703	bayes_pls_golite062009
N-methyltransferase activity	2.62081022854657	bayes_pls_golite062009
protein methyltransferase activity	2.60624413031692	bayes_pls_golite062009
transferase activity	1.97261773840408	bayes_pls_golite062009
rRNA methyltransferase activity	1.85380504590369	bayes_pls_golite062009
histone methyltransferase activity	1.5770892133978	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	1.5743030926483	bayes_pls_golite062009
arginine N-methyltransferase activity	1.17774160785818	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	1.17774160785818	bayes_pls_golite062009
catalytic activity	1.16970557486814	bayes_pls_golite062009
tRNA methyltransferase activity	0.752031224323003	bayes_pls_golite062009
binding	0.749096536959959	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	0.663671542316122	bayes_pls_golite062009
DNA (cytosine-5-)-methyltransferase activity	0.378599327496302	bayes_pls_golite062009
rRNA (uridine) methyltransferase activity	0.283627597838594	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	0.24819970199694	bayes_pls_golite062009
RNA binding	0.145125818301729	bayes_pls_golite062009
rRNA (adenine) methyltransferase activity	0.113328595762489	bayes_pls_golite062009
protein-arginine omega-N symmetric methyltransferase activity	0.0804008144859909	bayes_pls_golite062009
transcription regulator activity	0.0739385287924036	bayes_pls_golite062009
rRNA (adenine-N6,N6-)-dimethyltransferase activity	0.0390774859535424	bayes_pls_golite062009
DNA binding	0.029220083486074	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2