Protein Overview: NHX1

General Information:
Name(s) found:	NHX1 / YDR456W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|927695\|gb\|AAB64861.1\| Ydr456wp; CAI: 0.16 [Saccharomyces cerevisiae] [NCBI NR] gi\|6919935\|sp\|Q04121.1\|NHX1_YEAST RecName: Full=Endosomal/prevacuolar sodium/hydrogen exchanger; AltName: Full=Endosomal/prevacuolar Na(+)/H(+) exchanger; AltName: Full=Vacuolar protein sorting-associated protein 44; Flags: Precursor [NCBI NR] gi\|6320663\|ref\|NP_010744.1\| Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock; Nhx1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S69734 hypothetical protein YDR456w - yeast (Saccharomyces cerevisiae) [NCBI NR] Endosomal/prevacuolar sodium/hydrogen exchanger OS=Saccharomyces cerevisiae GN=NHX1 PE=1 SV=1 [Swiss-Prot] Mitochondrial sodium/hydrogen exchanger - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] NHX1 Na+/H+ exchanger of the prevacuolar compartment - involved in salt tolerance [mips] Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock [SGD] NHX1, Chr IV from 1367474-1369375 [yeast_orfs2] Chr IV from 1367474-1369375 [yeastorf3.fasta] Chr IV from 1367473-1369374 [yeastorf4.fasta] (Q04121) Mitochondrial sodium/hydrogen exchanger (Mitochondrial Na(+)/H(+) exchanger) [4932.FASTACCERV.txt] NHX1 SGDID:S0002864, Chr IV from 1367473-1369374, Verified ORF [yeastflysequest.fasta] NHX1 SGDID:S0002864, Chr IV from 1367475-1369376, Verified ORF [10112005cervSGD.fasta] Nhx1p [Saccharomyces cerevisiae]gi\|927695\|gb\|AAB64861.1\| Ydr456wp; CAI: 0.16 [Saccharomyces cerevisiae]gi\|2131504\|pir\|\|S69734 hypothetical protein YDR456w - yeast (Saccharomyces cerevisiae)gi\|6919935\|sp\|Q04121\|NAH2_YEAST Mitochondrial sodium/hydrogen e [nrdb_11152004_Chlamydomonas] NHX1 SGDID:S000002864, Chr IV from 1367475-1369376, Verified ORF, "Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NHX1 SGDID:S000002864, Chr IV from 1367477-1369378, Verified ORF, "Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock" [yeast-contam.fasta] NHX1 SGDID:S000002864, Chr IV from 1367478-1369379, Verified ORF, "Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Ydr456wp; CAI: 0.16 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock; Nhx1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial sodium/hydrogen exchanger (Mitochondrial Na(+)/H(+) exchanger) (Vacuolar protein sorting-associated protein 44) [nr-271106-contam.fasta] [chemostatdb.fasta] Endosomal/prevacuolar sodium/hydrogen exchanger OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NHX1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NHX1 SGDID:S000002864, Chr IV from 1367485-1369386, Genome Release 64-1-1, Verified ORF, "Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion" [yeast-030211-contam.fasta] NHX1 SGDID:S000002864, Chr IV from 1367485-1369386, Genome Release 64-2-1, Verified ORF, "Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism" [20141208_orf_trans.fasta] TR6162\|c0_g1_i1\|g.4380 ORF TR6162\|c0_g1_i1\|g.4380 TR6162\|c0_g1_i1\|m.4380 type:complete len:634 (+) TR6162\|c0_g1_i1:556-2457(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDR456W SGDID:S000002864, Chr IV from 1367485-1369386, Genome Release 64-1-1, Verified ORF, "Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_64\|g.851 ORF asmbl_64\|g.851 asmbl_64\|m.851 type:complete len:634 (+) asmbl_64:556-2457(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_64\|g.74 ORF ID=asmbl_64\|g.74 ID=asmbl_64\|m.74 type:complete len:634 (+) ID=asmbl_64:556-2457(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	633 amino acids
Gene Ontology:
Cellular Component:	late endosome [IDA]
Biological Process:	monovalent inorganic cation transport [IMP][ISS] endosome transport [IMP] vacuolar acidification [IMP] cellular monovalent inorganic cation homeostasis [IMP]
Molecular Function:	monovalent inorganic cation transmembrane transporter activity [IDA][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSKVLLNIA FKVLLTTAKR AVDPDDDDEL LPSPDLPGSD DPIAGDPDVD LNPVTEEMFS  60
   61 SWALFIMLLL LISALWSSYY LTQKRIRAVH ETVLSIFYGM VIGLIIRMSP GHYIQDTVTF 120
  121 NSSYFFNVLL PPIILNSGYE LNQVNFFNNM LSILIFAIPG TFISAVVIGI ILYIWTFLGL 180
  181 ESIDISFADA MSVGATLSAT DPVTILSIFN AYKVDPKLYT IIFGESLLND AISIVMFETC 240
  241 QKFHGQPATF SSVFEGAGLF LMTFSVSLLI GVLIGILVAL LLKHTHIRRY PQIESCLILL 300
  301 IAYESYFFSN GCHMSGIVSL LFCGITLKHY AYYNMSRRSQ ITIKYIFQLL ARLSENFIFI 360
  361 YLGLELFTEV ELVYKPLLII VAAISICVAR WCAVFPLSQF VNWIYRVKTI RSMSGITGEN 420
  421 ISVPDEIPYN YQMMTFWAGL RGAVGVALAL GIQGEYKFTL LATVLVVVVL TVIIFGGTTA 480
  481 GMLEVLNIKT GCISEEDTSD DEFDIEAPRA INLLNGSSIQ TDLGPYSDNN SPDISIDQFA 540
  541 VSSNKNLPNN ISTTGGNTFG GLNETENTSP NPARSSMDKR NLRDKLGTIF NSDSQWFQNF 600
  601 DEQVLKPVFL DNVSPSLQDS ATQSPADFSS QNH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		NHX1 STV1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	ASF1	3908: TAP-tagged, includes hir2(delta) in background	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SEC17	NHX1	1	Unpublished Fields Lab Data
View Screen	FCF2	NHX1	1	Unpublished Fields Lab Data
View Screen	UBC7	NHX1	1	Unpublished Fields Lab Data
View Screen	ARG1	NHX1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NHX1 and SNF7	Huh WK, et al. (2003)
View Data	NHX1	Huh WK, et al. (2003)

Protein Structure Data

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Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..506]	Pfam	140.387216	Sodium/hydrogen exchanger family No confident structure predictions are available.
2	View Details	[507..633]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2