Protein Overview: RTG3

General Information:
Name(s) found:	RTG3 / YBL103C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319365\|ref\|NP_009447.1\| Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways; Rtg3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S74319 RTG3 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1870091\|emb\|CAA84929.1\| RTG3 [Saccharomyces cerevisiae] [NCBI NR] gi\|1710804\|sp\|P38165.2\|RTG3_YEAST RecName: Full=Retrograde regulation protein 3 [NCBI NR] gi\|1177881\|gb\|AAA86842.1\| Rtg3p [NCBI NR] Retrograde regulation protein 3 OS=Saccharomyces cerevisiae GN=RTG3 PE=1 SV=2 [Swiss-Prot] RTG3 BHLH/zip transcription factor that regulates CIT2 gene expression [mips] Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways [SGD] RTG3, Chr II from 22070-23530, reverse complement [yeast_orfs2] Chr II from 22070-23530, reverse complement [yeastorf3.fasta] Chr II from 23530-22070, reverse complement [yeastorf5.fasta] (P38165) Retrograde regulation protein 3 [4932.FASTACCERV.txt] RTG3 SGDID:S0000199, Chr II from 23535-22075, reverse complement, Verified ORF [yeastflysequest.fasta] Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways [Saccharomyces cerevisiae]gi\|1870091\|emb\|CAA84929.1\| RTG3 [Saccharomyces ce [nrdb_11152004_Chlamydomonas] RTG3 SGDID:S000000199, Chr II from 23535-22075, reverse complement, Verified ORF, "Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pa [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Retrograde regulation protein 3 [nr-271106-contam.fasta] Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways; Rtg3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RTG3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Rtg3p [nr-271106-contam.fasta] RTG3 SGDID:S000000199, Chr II from 23535-22075, reverse complement, Verified ORF, "Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Retrograde regulation protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTG3 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RTG3 SGDID:S000000199, Chr II from 23536-22076, Genome Release 64-1-1, reverse complement, Verified ORF, "Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways" [yeast-030211-contam.fasta] RTG3 SGDID:S000000199, Chr II from 23536-22076, Genome Release 64-2-1, reverse complement, Verified ORF, "bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p" [20141208_orf_trans.fasta] YBL103C SGDID:S000000199, Chr II from 23536-22076, Genome Release 64-1-1, reverse complement, Verified ORF, "Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	486 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	mitochondria-nucleus signaling pathway [IDA] transcription initiation from RNA polymerase II promoter [IDA]
Molecular Function:	specific RNA polymerase II transcription factor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMNNNESEAE NQRLLDELMN QTKVLQETLD FSLVTPTPHH NDDYKIHGSA YPGGETPAQQ  60
   61 HEKLSYINTH NSNDNNNLMG SQARSNSQTP TASTIYEEAE SQSSYLDDMF RTSQGGRPVT 120
  121 QNSISSIGQG PLRSSYSMAY DSPVDRAMNT PLQQQEGLKA ELPHDFLFQH GTDDTMYNLT 180
  181 DDLSSSLSSS INSDMMTPNT YSSSFSYNPQ SLGPASVSST YSPKVRSPSS SFRAGSFLSS 240
  241 SFRHGSINTP RTRHTSISSN MTENIGPGSV PKILGGLTSD EKLRRKREFH NAVERRRREL 300
  301 IKQKIKELGQ LVPPSLLNYD DLGKQIKPNK GIILDRTVEY LQYLAEILEI QARKKKALLA 360
  361 KIKELEEKKS SVAALSPFTN NHHASSGQNN SENSEERIID IRSVPNALMN EQNSKAELHN 420
  421 WEPPLYDSVG NHNHAGTMES HPHTNIHEEL KEFLSGDLIE AEDNAKLMFG DDNSNPADYL 480
  481 LEFGSG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		RTG1 RTG3
View Details	(MIPS) Mewes HW, et al. (2004)		RTG1 RTG3
View Details	Krogan NJ, et al. (2006)		RTG1 RTG3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAS2	RTG3	2	Unpublished Fields Lab Data
View Screen	CDC48	RTG3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RTG3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..276]	MSA	35.347997	View MSA. No confident structure predictions are available.
2	View Details	[277..422]	PSI-BLAST	40.64593	SREBP-1a
3	View Details	[423..486]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2