YRC Public Data Repository - Protein Structure Prediction

Protein:	YEIT_ECOLI
Organism:	Escherichia coli
Length:	412 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for YEIT_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|20515680, gi\|... - gi\|20807180\|ref\|NP_622351.1\| NADPH-dependent glutamate synthase beta chain and related oxidoreductas... gi\|20807180 - gi\|20807180\|ref\|NP_622351.1\| NADPH-dependent glutamate synthase beta chain and related oxidoreductas...	365.0	[0..3]	[409..20]
gi\|94970706, gi\|... - gi\|94970706\|ref\|YP_592754.1\| glutamate synthase (NADH) small subunit [Candidatus Koribacter versatil...	365.0	[0..1]	[409..21]
gi\|90580606, gi\|... - gi\|90580606\|ref\|ZP_01236411.1\| glutamate synthase, small subunit [Vibrio angustum S14], gi\|90438264\|...	365.0	[0..3]	[407..24]
gi\|95108604, gi\|... - gi\|95108604\|emb\|CAK13298.1\| glutamate synthase [NADPH] small chain (GOGAT) [Pseudomonas entomophila ... gi\|95108604 - gi\|95108604\|emb\|CAK13298.1\| glutamate synthase [NADPH] small chain (GOGAT) [Pseudomonas entomophila ...	364.0	[0..6]	[407..30]
gi\|89072509, gi\|... - gi\|89072509\|ref\|ZP_01159081.1\| glutamate synthase, small subunit [Photobacterium sp. SKA34], gi\|8905...	364.0	[0..3]	[407..24]
gi\|53612313, gi\|... - gi\|67158022\|ref\|ZP_00419112.1\| Glutamate synthase, NADH/NADPH, small subunit 2 [Azotobacter vineland... gi\|67158022, gi\|... - gi\|67158022\|ref\|ZP_00419112.1\| Glutamate synthase, NADH/NADPH, small subunit 2 [Azotobacter vineland...	363.0	[0..6]	[406..30]
gi\|26991751, gi\|... - gi\|26991751\|ref\|NP_747176.1\| glutamate synthase subunit beta [Pseudomonas putida KT2440], gi\|2498685... gi\|82716770, gi\|... - gi\|82736127\|ref\|ZP_00898987.1\| Glutamate synthase, NADH/NADPH, small subunit 2 [Pseudomonas putida F... gi\|58615307 - gi\|58615307\|gb\|AAW80264.1\| GltD [Pseudomonas putida] tr\|E4RAK0\|E4RAK0... - GltD OS=Pseudomonas putida (strain BIRD-1) GN=gltD PE=4 SV=1 gi\|167036114, gi... - gi\|167036114\|ref\|YP_001671345.1\| glutamate synthase subunit beta [Pseudomonas putida GB-1], gi\|16686...	363.0	[0..6]	[407..30]
gi\|167036646, gi... - gi\|76797438\|ref\|ZP_00779755.1\| Glutamate synthase (NADPH), homotetrameric [Thermoanaerobacter ethano... gi\|241903592, gi... - gi\|241903592\|ref\|ZP_04788313.1\| glutamate synthase (NADPH), homotetrameric [Thermoanaerobacter brock...	363.0	[0..3]	[409..20]
gi\|89951927, gi\|... - gi\|90022327\|ref\|YP_528154.1\| glutamate synthase subunit beta [Saccharophagus degradans 2-40], gi\|899...	362.0	[0..3]	[407..38]
gi\|9951324, gi\|1... - gi\|9951324\|gb\|AAG08420.1\|AE004916_9 glutamate synthase small chain [Pseudomonas aeruginosa PAO1], gi... gi\|218894134, gi... - gi\|218894134\|ref\|YP_002443003.1\| glutamate synthase subunit beta [Pseudomonas aeruginosa LESB58], gi... gi\|53726581, gi\|... - gi\|53726581\|ref\|ZP_00141511.2\| COG0493: NADPH-dependent glutamate synthase beta chain and related ox... gi\|107104135 - gi\|107104135\|ref\|ZP_01368053.1\| hypothetical protein PaerPA_01005208 [Pseudomonas aeruginosa PACS2] gi\|1750398, gi\|1... - gi\|1750398\|gb\|AAB39260.1\| glutamate synthase small subunit [Pseudomonas aeruginosa], pir\|\|G83017 glu...	362.0	[0..2]	[406..31]

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Predicted Domain #1

Region A:
Residues: [1-412]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS IYFRNFKGAA  60
   61 ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR LQRFVTDFEQ QTGMEIYQPG 120
  121 TKTLGKVAII GAGPAGLQAS VTLTNQGYDV TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE 180
  181 IARIEKMGVT IKCNNEVGNT LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL 240
  241 QRARQAQGDI SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS 300
  301 ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL DAFAELEPQR 360
  361 NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE AIHHYLEGAC SC

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	61.221849
Match:	1h7xA
Description:	Dihydropyrimidine dehydrogenase, N-terminal domain; Dihydropyrimidine dehydrogenase, domain 4; Dihydropyrimidine dehydrogenase, domain 3; Dihydropyrimidine dehydrogenase, domain 2; Dihydropyrimidine dehydrogenase, C-terminal domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glutamate synthase activity	8.25305391467034	bayes_pls_golite062009
glutamate synthase activity, NADH or NADPH as acceptor	8.23578981764913	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor	7.02480361242491	bayes_pls_golite062009
glutamate synthase (NADPH) activity	6.79992270958953	bayes_pls_golite062009
succinate dehydrogenase activity	1.80846474206463	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	1.45813178614374	bayes_pls_golite062009
catalytic activity	1.35682608895635	bayes_pls_golite062009
oxidoreductase activity	1.32475122976745	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor	0.384435272305167	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	0.201800390587271	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: