YRC Public Data Repository - Protein Structure Prediction

Protein:	NIFJ_ECOLI
Organism:	Escherichia coli
Length:	1174 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NIFJ_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|20806954 - gi\|20806954\|ref\|NP_622125.1\| Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxi... gi\|20515433, gi\|... - gi\|20806954\|ref\|NP_622125.1\| pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxi...	1012.0	[0..1]	[1168..5]
gi\|91072216, gi\|... - gi\|91210642\|ref\|YP_540628.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli UTI89], gi\|9107221... gi\|237705378, gi... - gi\|237705378\|ref\|ZP_04535859.1\| pyruvate-flavodoxin oxidoreductase [Escherichia sp. 3_2_53FAA], gi\|2... gi\|117623652, gi... - gi\|117623652\|ref\|YP_852565.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli APEC O1], gi\|1155... gi\|218365103, gi... - gi\|218558334\|ref\|YP_002391247.1\| putative 2-oxoacid-flavodoxin fused oxidoreductase:conserved protei...	1010.0	[0..1]	[1174..1]
gi\|194419782, gi... - gi\|83571039\|ref\|ZP_00922481.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer...	1009.0	[0..1]	[1174..1]
gi\|110343175, gi... - gi\|110641583\|ref\|YP_669313.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli 536], gi\|11034317... gi\|75242146, gi\|... - gi\|75242146\|ref\|ZP_00725940.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer...	1009.0	[0..1]	[1174..1]
gi\|191165148, gi... - gi\|75231854\|ref\|ZP_00718195.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer...	1007.0	[0..1]	[1174..1]
gi\|124527899, gi... - gi\|124527899\|ref\|ZP_01699288.1\| pyruvate flavodoxin/ferredoxin oxidoreductase domain protein [Escher... gi\|75195495, gi\|... - gi\|75195495\|ref\|ZP_00705565.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer... gi\|169755215, gi... - gi\|170020287\|ref\|YP_001725241.1\| pyruvate flavodoxin/ferredoxin oxidoreductase domain-containing pro...	1006.0	[0..1]	[1174..1]
gi\|75259503, gi\|... - gi\|75259503\|ref\|ZP_00730822.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer... gi\|192959596, gi... - gi\|75239990\|ref\|ZP_00723951.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer... gi\|157155711, gi... - gi\|75190435\|ref\|ZP_00703702.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer...	1006.0	[0..1]	[1174..1]
gi\|194424662, gi... - gi\|83587751\|ref\|ZP_00926377.1\| COG0674: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:fer...	1006.0	[0..1]	[1174..1]
gi\|13361465, gi\|... - gi\|15831254\|ref\|NP_310027.1\| putative oxidoreductase [Escherichia coli O157:H7 str. Sakai], gi\|13361... gi\|217317716, gi... - gi\|217329277\|ref\|ZP_03445357.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli O157:H7 str. TW... gi\|25284482, gi\|... - pir\|\|H90878 probable oxidoreductase ECs2000 [imported] - Escherichia coli (strain O157:H7, substrain... gi\|13361465, gi\|... - gi\|15831254\|ref\|NP_310027.1\| putative oxidoreductase [Escherichia coli O157:H7], gi\|13361465\|dbj\|BAB... gi\|189366638, gi... - gi\|189366638\|gb\|EDU85054.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli O157:H7 str. EC4501...	1005.0	[0..1]	[1174..1]
gi\|26247685, gi\|... - gi\|26247685\|ref\|NP_753725.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli CFT073], gi\|261080... gi\|227836417, gi... - gi\|227886213\|ref\|ZP_04004018.1\| pyruvate-flavodoxin oxidoreductase [Escherichia coli 83972], gi\|2278...	1005.0	[0..1]	[1174..1]

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Predicted Domain #1

Region A:
Residues: [1-1174]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MITIDGNGAV ASVAFRTSEV IAIYPITPSS TMAEQADAWA GNGLKNVWGD TPRVVEMQSE  60
   61 AGAIATVHGA LQTGALSTSF TSSQGLLLMI PTLYKLAGEL TPFVLHVAAR TVATHALSIF 120
  121 GDHSDVMAVR QTGCAMLCAA NVQEAQDFAL ISQIATLKSR VPFIHFFDGF RTSHEINKIV 180
  181 PLADDTILDL MPQVEIDAHR ARALNPEHPV IRGTSANPDT YFQSREATNP WYNAVYDHVE 240
  241 QAMNDFSAAT GRQYQPFEYY GHPQAERVII LMGSAIGTCE EVVDELLTRG EKVGVLKVRL 300
  301 YRPFSAKHLL QALPGSVRSV AVLDRTKEPG AQAEPLYLDV MTALAEAFNN GERETLPRVI 360
  361 GGRYGLSSKE FGPDCVLAVF AELNAAKPKA RFTVGIYDDV TNLSLPLPEN TLPNSAKLEA 420
  421 LFYGLGSDGS VSATKNNIKI IGNSTPWYAQ GYFVYDSKKA GGLTVSHLRV SEQPIRSAYL 480
  481 ISQADFVGCH QLQFIDKYQM AERLKPGGIF LLNTPYSADE VWSRLPQEVQ AVLNQKKARF 540
  541 YVINAAKIAR ECGLAARINT VMQMAFFHLT QILPGDSALA ELQGAIAKSY SSKGQDLVER 600
  601 NWQALALARE SVEEVPLQPV NPHSANRPPV VSDAAPDFVK TVTAAMLAGL GDALPVSALP 660
  661 PDGTWPMGTT RWEKRNIAEE IPIWKEELCT QCNHCVAACP HSAIRAKVVP PEAMENAPAS 720
  721 LHSLDVKSRD MRGQKYVLQV APEDCTGCNL CVEVCPAKDR QNPEIKAINM MSRLEHVEEE 780
  781 KINYDFFLNL PEIDRSKLER IDIRTSQLIT PLFEYSGACS GCGETPYIKL LTQLYGDRML 840
  841 IANATGCSSI YGGNLPSTPY TTDANGRGPA WANSLFEDNA EFGLGFRLTV DQHRVRVLRL 900
  901 LDQFADKIPA ELLTALKSDA TPEVRREQVA ALRQQLNDVA EAHELLRDAD ALVEKSIWLI 960
  961 GGDGWAYDIG FGGLDHVLSL TENVNILVLD TQCYSNTGGQ ASKATPLGAV TKFGEHGKRK1020
 1021 ARKDLGVSMM MYGHVYVAQI SLGAQLNQTV KAIQEAEAYP GPSLIIAYSP CEEHGYDLAL1080
 1081 SHDQMRQLTA TGFWPLYRFD PRRADEGKLP LALDSRPPSE APEETLLHEQ RFRRLNSQQP1140
 1141 EVAEQLWKDA AADLQKRYDF LAQMAGKAEK SNTD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1000.0
Match:	1b0pA
Description:	Pyruvate-ferredoxin oxidoreductase, PFOR, domain I; Pyruvate-ferredoxin oxidoreductase, PFOR, domains VI; Pyruvate-ferredoxin oxidoreductase, PFOR, domain II; Pyruvate-ferredoxin oxidoreductase, PFOR, domain III; Pyruvate-ferredoxin oxidoreductase, PFOR, domain V
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
pyruvate dehydrogenase activity	4.78378116511208	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	4.60815384927294	bayes_pls_golite062009
pyruvate dehydrogenase (acetyl-transferring) activity	4.53835330045346	bayes_pls_golite062009
catalytic activity	2.62581591393304	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	1.71446964011957	bayes_pls_golite062009
NADH dehydrogenase activity	1.52708302196048	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	1.34194069643424	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	1.33446932713773	bayes_pls_golite062009
transketolase activity	1.17934016977965	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	1.01522839124481	bayes_pls_golite062009
NADH dehydrogenase (ubiquinone) activity	1.00909748207872	bayes_pls_golite062009
oxidoreductase activity	0.716854888534	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.62952479827273	bayes_pls_golite062009
formate dehydrogenase activity	0.13956405004608	bayes_pls_golite062009
acetolactate synthase activity	0.10785955428579	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: