YRC Public Data Repository - Protein Structure Prediction

Protein:	GLCF_ECOLI
Organism:	Escherichia coli
Length:	407 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GLCF_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|882507, gi\|74... - gi\|882507\|gb\|AAA69145.1\| ORF_f761, gi\|7451596\|pir\|\|H65083 glycolate oxidase subunits GlcE and GlcF -... gi\|16130878\|ref\|... - glycolate oxidase iron-sulfur subunit [Escherichia coli K12], glycolate oxidase iron-sulfur subunit ... gi\|882507\|gb\|AAA... - ORF_f761	332.0	[0..1]	[407..355]
gi\|227835073, gi... - gi\|227887677\|ref\|ZP_04005482.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli 83972], gi\|2... gi\|110344801, gi... - gi\|110643209\|ref\|YP_670939.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli 536], gi\|11034... gi\|47155044 - gi\|47155044\|emb\|CAE85243.1\| GlcF protein [Escherichia coli] gi\|218701746, gi... - gi\|218701746\|ref\|YP_002409375.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli IAI39], gi\|...	324.0	[0..1]	[407..1]
gi\|48768629, gi\|... - gi\|94311861\|ref\|YP_585071.1\| glycolate oxidase iron-sulfur subunit [Ralstonia metallidurans CH34], g...	323.0	[0..1]	[404..1]
gi\|191169574, gi... - gi\|75227064\|ref\|ZP_00713889.1\| COG0247: Fe-S oxidoreductase [Escherichia coli B7A], gi\|191169574\|ref... gi\|157077628, gi... - gi\|75187792\|ref\|ZP_00701059.1\| COG0247: Fe-S oxidoreductase [Escherichia coli E24377A], gi\|157155598...	323.0	[0..1]	[407..1]
gi\|193067448, gi... - gi\|75239446\|ref\|ZP_00723417.1\| COG0247: Fe-S oxidoreductase [Escherichia coli E110019], gi\|193067448...	322.0	[0..1]	[407..1]
gi\|75259916, gi\|... - gi\|75259916\|ref\|ZP_00731194.1\| COG0247: Fe-S oxidoreductase [Escherichia coli E22], gi\|193061936\|ref... gi\|218353414, gi... - gi\|218696682\|ref\|YP_002404349.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli 55989], gi\|... gi\|209913705, gi... - gi\|209920446\|ref\|YP_002294530.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli SE11], gi\|2...	322.0	[0..1]	[407..1]
gi\|194436683, gi... - gi\|83587583\|ref\|ZP_00926210.1\| COG0247: Fe-S oxidoreductase [Escherichia coli 101-1], gi\|194436683\|r...	322.0	[0..1]	[407..1]
gi\|218560046, gi... - gi\|218560046\|ref\|YP_002392959.1\| glycolate oxidase iron-sulfur subunit [Escherichia coli S88], gi\|21... gi\|75241175, gi\|... - gi\|75241175\|ref\|ZP_00725037.1\| COG0247: Fe-S oxidoreductase [Escherichia coli F11], gi\|191171853\|ref... gi\|226899322, gi... - gi\|237706282\|ref\|ZP_04536763.1\| glycolate oxidase iron-sulfur subunit [Escherichia sp. 3_2_53FAA], g...	321.0	[0..1]	[407..1]
gi\|90335993, gi\|... - gi\|90419952\|ref\|ZP_01227861.1\| glycolate oxidase, iron-sulfur subunit [Aurantimonas sp. SI85-9A1], g... gi\|90335993, gi\|... - gi\|90419952\|ref\|ZP_01227861.1\| glycolate oxidase, iron-sulfur subunit [Aurantimonas sp. SI85-9A1], g...	321.0	[0..1]	[407..1]
gi\|67734885, gi\|... - gi\|67734885\|ref\|ZP_00485952.1\| COG0247: Fe-S oxidoreductase [Burkholderia pseudomallei 668], gi\|1264...	319.0	[0..1]	[406..1]

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Predicted Domain #1

Region A:
Residues: [1-299]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQTQLTEEMR QNARALEADS ILRACVHCGF CTATCPTYQL LGDELDGPRG RIYLIKQVLE  60
   61 GNEVTLKTQE HLDRCLTCRN CETTCPSGVR YHNLLDIGRD IVEQKVKRPL PERILREGLR 120
  121 QVVPRPAVFR ALTQVGLVLR PFLPEQVRAK LPAETVKAKP RPPLRHKRRV LMLEGCAQPT 180
  181 LSPNTNAATA RVLDRLGISV MPANEAGCCG AVDYHLNAQE KGLARARNNI DAWWPAIEAG 240
  241 AEAILQTASG CGAFVKEYGQ MLKNDALYAD KARQVSELAV DLVELLREEP LEKLAIRGD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	16.30103
Match:	1b0pA
Description:	Pyruvate-ferredoxin oxidoreductase, PFOR, domain I; Pyruvate-ferredoxin oxidoreductase, PFOR, domains VI; Pyruvate-ferredoxin oxidoreductase, PFOR, domain II; Pyruvate-ferredoxin oxidoreductase, PFOR, domain III; Pyruvate-ferredoxin oxidoreductase, PFOR, domain V
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
formate dehydrogenase activity	4.7946737640663	bayes_pls_golite062009
NADH dehydrogenase activity	3.13953145151561	bayes_pls_golite062009
NADH dehydrogenase (ubiquinone) activity	2.76338281383262	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	2.71230504811675	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	2.70037803782769	bayes_pls_golite062009
electron carrier activity	2.45164586846912	bayes_pls_golite062009
transketolase activity	2.14943266725938	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	2.09041948600744	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	1.80621486978907	bayes_pls_golite062009
4 iron, 4 sulfur cluster binding	1.5619384041388	bayes_pls_golite062009
pyruvate dehydrogenase activity	1.51399046206095	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	1.50909082807368	bayes_pls_golite062009
metal cluster binding	1.48723437664337	bayes_pls_golite062009
iron-sulfur cluster binding	1.48723437664337	bayes_pls_golite062009
pyruvate dehydrogenase (acetyl-transferring) activity	1.33926757092229	bayes_pls_golite062009
oxidoreductase activity	0.660370448422897	bayes_pls_golite062009
catalytic activity	0.488483980994331	bayes_pls_golite062009
acetolactate synthase activity	0.10785955428579	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	0.0827182678048914	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [300-407]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKLAFHCPCT LQHAQKLNGE VEKVLLRLGF TLTDVPDSHL CCGSAGTYAL THPDLARQLR  60
   61 DNKMNALESG KPEMIVTANI GCQTHLASAG RTSVRHWIEI VEQALEKE

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	16.119186
Match:	PF02754.7
Description:	No description for PF02754.7 was found.

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: