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View Structure Prediction Details

Protein: TfIIFalpha-PA
Organism: Drosophila melanogaster
Length: 577 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TfIIFalpha-PA.

Description E-value Query
Range
Subject
Range
gi|73987252 - gi|73987252|ref|XP_533935.2| PREDICTED: similar to Transcription initiation factor IIF alpha subunit...
213.0 [0..9] [577..77]

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Predicted Domain #1
Region A:
Residues: [1-194]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSASKSTPS AASGSSTSAA AAAAASVASG SASSSANVQE FKIRVPKMPK KHHVMRFNAT  60
   61 LNVDFAQWRN VKLERENNMK EFRGMEEDQP KFGAGSEYNR DQREEARRKK FGIIARKYRP 120
  121 EAQPWILKVG GKTGKKFKGI REGGVGENAA FYVFTHAPDG AIEAYPLTEW YNFQPIQRYK 180
  181 SLSAEEAEQE FGRR

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 38.30103
Match: 1f3uB
Description: TFIIF alpha subunit, Rap74
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [195-285]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKVMNYFSLM LRKRLRGDEE EEQDPEEAKL IKAATKKSKE LKITDMDEWI DSEDESDSED  60
   61 EEDKKKKEQE DSDDGKAKGK GKKGADKKKK K

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3
Region A:
Residues: [286-463]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RDVDDEAFEE SDDGDEEGRE MDYDTSSSED EPDPEAKVDK DMKGVAEEDA LRKLLTSDEE  60
   61 EDDEKKSDES DKEDADGEKK KKDKGKDEVS KDKKKKKPTK DDKKGKSNGS GDSSTDFSSD 120
  121 STDSEDDLSN GPPKKKVVVK DKDKEKEKEK ESAASSKVIA SSSNANKSRS ATPTLSTD

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [464-577]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ASKRKMNSLP SDLTASDTSN SPTSTPAKRP KNEISTSLPT SFSGGKVEDY GITEEAVRRY  60
   61 LKRKPLTATE LLTKFKNKKT PVSSDRLVET MTKILKKINP VKHTIQGKMY LWIK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 11.0
Match: 1nhaA
Description: C-terminal domain of the rap74 subunit of TFIIF
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 0.835523092977445 bayes_pls_golite062009
transcription regulator activity 0.4843036104266 bayes_pls_golite062009
nucleic acid binding 0.37117756729668 bayes_pls_golite062009
DNA binding 0.3118855114645 bayes_pls_golite062009

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle