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View Structure Prediction Details

Protein: TfIIFalpha-PA
Organism: Drosophila melanogaster
Length: 577 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TfIIFalpha-PA.

Description E-value Query
Range
Subject
Range
gi|73987252 - gi|73987252|ref|XP_533935.2| PREDICTED: similar to Transcription initiation factor IIF alpha subunit...
213.0 [0..9] [577..77]

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Predicted Domain #1
Region A:
Residues: [1-194]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSASKSTPS AASGSSTSAA AAAAASVASG SASSSANVQE FKIRVPKMPK KHHVMRFNAT  60
   61 LNVDFAQWRN VKLERENNMK EFRGMEEDQP KFGAGSEYNR DQREEARRKK FGIIARKYRP 120
  121 EAQPWILKVG GKTGKKFKGI REGGVGENAA FYVFTHAPDG AIEAYPLTEW YNFQPIQRYK 180
  181 SLSAEEAEQE FGRR

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 38.30103
Match: 1f3uB
Description: TFIIF alpha subunit, Rap74
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [195-285]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKVMNYFSLM LRKRLRGDEE EEQDPEEAKL IKAATKKSKE LKITDMDEWI DSEDESDSED  60
   61 EEDKKKKEQE DSDDGKAKGK GKKGADKKKK K

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #3
Region A:
Residues: [286-463]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RDVDDEAFEE SDDGDEEGRE MDYDTSSSED EPDPEAKVDK DMKGVAEEDA LRKLLTSDEE  60
   61 EDDEKKSDES DKEDADGEKK KKDKGKDEVS KDKKKKKPTK DDKKGKSNGS GDSSTDFSSD 120
  121 STDSEDDLSN GPPKKKVVVK DKDKEKEKEK ESAASSKVIA SSSNANKSRS ATPTLSTD

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [464-577]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ASKRKMNSLP SDLTASDTSN SPTSTPAKRP KNEISTSLPT SFSGGKVEDY GITEEAVRRY  60
   61 LKRKPLTATE LLTKFKNKKT PVSSDRLVET MTKILKKINP VKHTIQGKMY LWIK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 11.0
Match: 1nhaA
Description: C-terminal domain of the rap74 subunit of TFIIF
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 0.835523092977445 bayes_pls_golite062009
transcription regulator activity 0.4843036104266 bayes_pls_golite062009
nucleic acid binding 0.37117756729668 bayes_pls_golite062009
DNA binding 0.3118855114645 bayes_pls_golite062009

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle