YRC Public Data Repository - Protein Structure Prediction

Protein:	GUS1
Organism:	Saccharomyces cerevisiae
Length:	708 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GUS1.

Description	E-value	Query Range	Subject Range
SYEC_YEAST - Glutamate--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GUS1... GUS1 - Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... gi\|190407178 - gi\|190407178\|gb\|EDV10445.1\| glutamyl-tRNA synthetase [Saccharomyces cerevisiae RM11-1a] gi\|151943574 - gi\|151943574\|gb\|EDN61884.1\| glutamyl-tRNA synthetase [Saccharomyces cerevisiae YJM789]	0.0	[1..708]	[1..708]
gi\|44984811, gi\|... - gi\|45198782\|ref\|NP_985811.1\| AFR264Wp [Ashbya gossypii ATCC 10895], gi\|44984811\|gb\|AAS53635.1\| AFR26... gi\|44984811 - gi\|44984811\|gb\|AAS53635.1\| AFR264Wp [Ashbya gossypii ATCC 10895]	0.0	[1..708]	[1..707]
gi\|27371005 - gi\|27371005\|gb\|AAH40802.1\| Eprs protein [Mus musculus]	0.0	[2..701]	[3..708]
gi\|46442213, gi\|... - gi\|77022500\|ref\|XP_888694.1\| hypothetical protein CaO19_7057 [Candida albicans SC5314], gi\|68471145\|... gi\|76573507 - gi\|76573507\|dbj\|BAE44591.1\| hypothetical protein [Candida albicans]	0.0	[1..708]	[1..725]
gi\|37589132 - gi\|37589132\|gb\|AAH58921.1\| EPRS protein [Homo sapiens]	0.0	[2..701]	[3..708]
SYQ_ECODH - Glutamine--tRNA ligase OS=Escherichia coli (strain K12 / DH10B) GN=glnS PE=3 SV=1 SYQ_ECO8A - Glutamine--tRNA ligase OS=Escherichia coli O8 (strain IAI1) GN=glnS PE=3 SV=1 SYQ_ECOSE - Glutamine--tRNA ligase OS=Escherichia coli (strain SE11) GN=glnS PE=3 SV=1 SYQ_ECO55 - Glutamine--tRNA ligase OS=Escherichia coli (strain 55989 / EAEC) GN=glnS PE=3 SV=1 EG10390 - glutamyl-tRNA synthetase / Glutamine--tRNA ligase SYQ_ECOBW - Glutamine--tRNA ligase OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=glnS PE=3 SV=1 gi\|251784170, gi... - gi\|251784170\|ref\|YP_002998474.1\| hypothetical protein B21_00628 [Escherichia coli BL21], gi\|24237644... gi\|254287536, gi... - gi\|254287536\|ref\|YP_003053284.1\| glutaminyl-tRNA synthetase [Escherichia coli BL21(DE3)], gi\|2539768... EG10390 - glutamyl-tRNA synthetase / Glutamine--tRNA ligase SYQ_SHISS - Glutamine--tRNA ligase OS=Shigella sonnei (strain Ss046) GN=glnS PE=3 SV=1 gi\|254160748, gi... - gi\|254160748\|ref\|YP_003043856.1\| glutaminyl-tRNA synthetase [Escherichia coli B str. REL606], gi\|253... SYQ_ECOHS - Glutamine--tRNA ligase OS=Escherichia coli O9:H4 (strain HS) GN=glnS PE=3 SV=1 SYQ_ECOLI - Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Glutamine--tRNA ligase) (GlnRS) - Escherichia coli gi\|192930998, gi... - gi\|75258594\|ref\|ZP_00730001.1\| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Escherichia coli ... SYQ_ECOLC - Glutamine--tRNA ligase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=glnS PE=3 SV=1 gi\|124529704, gi... - gi\|124529704\|ref\|ZP_01700611.1\| glutaminyl-tRNA synthetase [Escherichia coli B], gi\|124499707\|gb\|EAY... gi\|89107538, gi\|... - gi\|89107538\|ref\|AP_001318.1\| glutamyl-tRNA synthetase [Escherichia coli str. K-12 substr. W3110], gi... SYQ_ECO24 - Glutamine--tRNA ligase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=glnS PE=3 SV=1	0.0	[175..706]	[1..554]
gi\|1942956 - gi\|1942956\|pdb\|1QRT\|A Chain A, Glutaminyl-Trna Synthetase Mutant D235g Complexed With Glutamine Tran...	0.0	[181..706]	[6..553]

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Predicted Domain #1

Region A:
Residues: [1-177]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPSTLTINGK APIVAYAELI AARIVNALAP NSIAIKLVDD KKAPAAKLDD ATEDVFNKIT  60
   61 SKFAAIFDNG DKEQVAKWVN LAQKELVIKN FAKLSQSLET LDSQLNLRTF ILGGLKYSAA 120
  121 DVACWGALRS NGMCGSIIKN KVDVNVSRWY TLLEMDPIFG EAHDFLSKSL LELKKSA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	8.30103
Match:	1pn9A_
Description:	Crystal structure of an insect delta-class glutathione S-transferase from a DDT-resistant strain of the malaria vector Anopheles gambiae
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [178-708]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NVGKKKETHK ANFEIDLPDA KMGEVVTRFP PEPSGYLHIG HAKAALLNQY FAQAYKGKLI  60
   61 IRFDDTNPSK EKEEFQDSIL EDLDLLGIKG DRITYSSDYF QEMYDYCVQM IKDGKAYCDD 120
  121 TPTEKMREER MDGVASARRD RSVEENLRIF TEEMKNGTEE GLKNCVRAKI DYKALNKTLR 180
  181 DPVIYRCNLT PHHRTGSTWK IYPTYDFCVP IVDAIEGVTH ALRTIEYRDR NAQYDWMLQA 240
  241 LRLRKVHIWD FARINFVRTL LSKRKLQWMV DKDLVGNWDD PRFPTVRGVR RRGMTVEGLR 300
  301 NFVLSQGPSR NVINLEWNLI WAFNKKVIDP IAPRHTAIVN PVKIHLEGSE APQEPKIEMK 360
  361 PKHKKNPAVG EKKVIYYKDI VVDKDDADVI NVDEEVTLMD WGNVIITKKN DDGSMVAKLN 420
  421 LEGDFKKTKH KLTWLADTKD VVPVDLVDFD HLITKDRLEE DESFEDFLTP QTEFHTDAIA 480
  481 DLNVKDMKIG DIIQFERKGY YRLDALPKDG KPYVFFTIPD GKSVNKYGAK K

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	150.0
Match:	1o0bA_
Description:	Gln-tRNA synthetase (GlnRS), C-terminal (anticodon-binding) domain; Glutaminyl-tRNA synthetase (GlnRS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
glutamate-tRNA ligase activity	5.13420185144914	bayes_pls_golite062009
structural constituent of ribosome	4.61484562096458	bayes_pls_golite062009
glutamine-tRNA ligase activity	4.45248364270113	bayes_pls_golite062009
structural molecule activity	4.261982318682	bayes_pls_golite062009
methionine-tRNA ligase activity	2.56893064485672	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
5S rRNA binding	2.25646775578129	bayes_pls_golite062009
rRNA binding	2.12571610259264	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
nucleic acid binding	1.64840822019417	bayes_pls_golite062009
arginine-tRNA ligase activity	1.50161401062914	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
binding	1.11291710031577	bayes_pls_golite062009
nucleotidyltransferase activity	1.06535308346284	bayes_pls_golite062009
cysteine-tRNA ligase activity	0.844943947059557	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: