YRC Public Data Repository - Protein Structure Prediction

Protein:	HTS1
Organism:	Saccharomyces cerevisiae
Length:	546 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HTS1.

Description	E-value	Query Range	Subject Range
HTS1 - Cytoplasmic and mitochondrial histidine tRNA synthetase; encoded by a single nuclear gene that speci... gi\|207340386 - gi\|207340386\|gb\|EDZ68752.1\| YPR033Cp-like protein [Saccharomyces cerevisiae AWRI1631] SYH_YEAST - Histidine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HT...	0.0	[1..546]	[1..546]
SYT_PSESM - Threonine--tRNA ligase OS=Pseudomonas syringae pv. tomato (strain DC3000) GN=thrS PE=3 SV=1 gi\|213970495, gi... - gi\|213970495\|ref\|ZP_03398622.1\| threonyl-tRNA synthetase [Pseudomonas syringae pv. tomato T1], gi\|21... SYT_PSESM - Threonyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=thrS PE=3 SV=1	0.0	[3..399]	[207..596]
SYT_VIBCM - Threonine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain M66-2) GN=thrS PE=3 SV=1 SYT_VIBCH - Threonine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN=t... gi\|229353090, gi... - gi\|229510645\|ref\|ZP_04400125.1\| threonyl-tRNA synthetase [Vibrio cholerae B33], gi\|229353090\|gb\|EEO1... gi\|229356910, gi... - gi\|229506559\|ref\|ZP_04396068.1\| threonyl-tRNA synthetase [Vibrio cholerae BX 330286], gi\|229356910\|g... gi\|229606038, gi... - gi\|229606038\|ref\|YP_002876742.1\| threonyl-tRNA synthetase [Vibrio cholerae MJ-1236], gi\|229372524\|gb... gi\|229345260, gi... - gi\|229517224\|ref\|ZP_04406669.1\| threonyl-tRNA synthetase [Vibrio cholerae RC9], gi\|229345260\|gb\|EEO1... gi\|126511981, gi... - gi\|153817556\|ref\|ZP_01970223.1\| threonyl-tRNA synthetase [Vibrio cholerae NCTC 8457], gi\|126511981\|g... gi\|153221646, gi... - gi\|153221646\|ref\|ZP_01952571.1\| threonyl-tRNA synthetase [Vibrio cholerae MAK 757], gi\|124119556\|gb\|... gi\|254850514, gi... - gi\|254850514\|ref\|ZP_05239864.1\| threonyl-tRNA synthetase [Vibrio cholerae MO10], gi\|254846219\|gb\|EET... SYT_VIBCH - Threonine--tRNA ligase OS=Vibrio cholerae GN=thrS PE=3 SV=1	0.0	[3..399]	[209..598]
SYT_PSEAE - Threonine--tRNA ligase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 1222... gi\|126194575, gi... - gi\|254241197\|ref\|ZP_04934519.1\| threonyl-tRNA synthetase [Pseudomonas aeruginosa 2192], gi\|194552182... SYT_PSEAB - Threonine--tRNA ligase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=thrS PE=3 SV=1 SYT_PSEAE - Threonyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=thrS PE=3 SV=1 SYT_PSEA8 - Threonine--tRNA ligase OS=Pseudomonas aeruginosa (strain LESB58) GN=thrS PE=3 SV=1	0.0	[3..399]	[207..596]
gi\|197241161, gi... - gi\|197262906\|ref\|ZP_03162980.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... SYT_SALTY - Threonine--tRNA ligase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=thrS PE=3 ... SYT_SALHS - Threonine--tRNA ligase OS=Salmonella heidelberg (strain SL476) GN=thrS PE=3 SV=1 SYT_SALTY - Threonyl-tRNA synthetase OS=Salmonella typhimurium GN=thrS PE=3 SV=1 gi\|168244449, gi... - gi\|205336802\|gb\|EDZ23566.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar He... gi\|205329759, gi... - gi\|205329759\|gb\|EDZ16523.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar 4,... lcl\|NC_016856.1_... - [gene=thrS] [protein=threonyl-tRNA synthetase] [protein_id=YP_005237194.1] [location=1422178..142410... SYT_SALTY - Threonine--tRNA ligase OS=Salmonella typhimurium GN=thrS PE=3 SV=1	0.0	[3..399]	[208..597]
SYT_SALTI - Threonyl-tRNA synthetase OS=Salmonella typhi GN=thrS PE=3 SV=1 gi\|199602680, gi... - gi\|200390235\|ref\|ZP_03216846.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... SYT_SALA4 - Threonine--tRNA ligase OS=Salmonella agona (strain SL483) GN=thrS PE=3 SV=1 SYT_SALSV - Threonine--tRNA ligase OS=Salmonella schwarzengrund (strain CVM19633) GN=thrS PE=3 SV=1 gi\|205341550, gi... - gi\|205341550\|gb\|EDZ28314.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar We... gi\|213852224 - gi\|213852224\|ref\|ZP_03381756.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213426331 - gi\|213426331\|ref\|ZP_03359081.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213423451 - gi\|213423451\|ref\|ZP_03356434.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|204927743, gi... - gi\|204927743\|ref\|ZP_03218944.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|168239001, gi... - gi\|197288252\|gb\|EDY27637.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Sc... SYT_SALTI - Threonine--tRNA ligase OS=Salmonella typhi GN=thrS PE=3 SV=1 gi\|167552305, gi... - gi\|205323017\|gb\|EDZ10856.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Sa... gi\|29141677, gi\|... - gi\|29141677\|ref\|NP_805019.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... gi\|16760562 - gi\|16760562\|ref\|NP_456179.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... gi\|238910851 - gi\|238910851\|ref\|ZP_04654688.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova...	0.0	[3..399]	[208..597]
SYT_PASMU - Threonyl-tRNA synthetase OS=Pasteurella multocida GN=thrS PE=3 SV=1 SYT_PASMU - Threonine--tRNA ligase OS=Pasteurella multocida (strain Pm70) GN=thrS PE=3 SV=1	0.0	[3..399]	[208..597]
SYT_ECOK1 - Threonine--tRNA ligase OS=Escherichia coli O1:K1 / APEC GN=thrS PE=3 SV=1 SYT_ECOLI - Threonyl-tRNA synthetase (EC 6.1.1.3) (Threonine--tRNA ligase) (ThrRS) - Escherichia coli SYT_SHISS - Threonine--tRNA ligase OS=Shigella sonnei (strain Ss046) GN=thrS PE=3 SV=1 SYT_SHIFL - Threonine--tRNA ligase OS=Shigella flexneri GN=thrS PE=3 SV=1 gi\|24112885, gi\|... - gi\|24112885\|ref\|NP_707395.1\| threonyl-tRNA synthetase [Shigella flexneri 2a str. 301], gi\|24051829\|g... EG11001 - threonyl-tRNA synthetase / Threonine--tRNA ligase, autogenously regulated; binds Zn(II) SYT_ECOL6 - Threonine--tRNA ligase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=thrS PE=3 S... gi\|30063014, gi\|... - gi\|30063014\|ref\|NP_837185.1\| threonyl-tRNA synthetase [Shigella flexneri 2a str. 2457T], gi\|30041263... SYT_ECOL6 - Threonine--tRNA ligase OS=Escherichia coli O6 GN=thrS PE=3 SV=1 sp\|B7USA2\|SYT_ECO27 - Threonyl-tRNA synthetase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=thrS PE=3 SV=1 SYT_ECOSM - Threonine--tRNA ligase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=thrS PE=3 SV=1 SYT_ECODH - Threonine--tRNA ligase OS=Escherichia coli (strain K12 / DH10B) GN=thrS PE=3 SV=1 gi\|190908082, gi... - gi\|191171752\|ref\|ZP_03033299.1\| threonyl-tRNA synthetase [Escherichia coli F11], gi\|190908082\|gb\|EDV... gi\|188492265, gi... - gi\|188492265\|ref\|ZP_02999535.1\| threonyl-tRNA synthetase [Escherichia coli 53638], gi\|188487464\|gb\|E... gi\|89108559, gi\|... - gi\|89108559\|ref\|AP_002339.1\| threonyl-tRNA synthetase [Escherichia coli str. K-12 substr. W3110], gi... SYT_ECO7I - Threonine--tRNA ligase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=thrS PE=3 SV=1 gi\|242377442, gi... - gi\|251785169\|ref\|YP_002999473.1\| hypothetical protein B21_01677 [Escherichia coli BL21], gi\|24237744... SYT_ECOUT - Threonine--tRNA ligase OS=Escherichia coli (strain UTI89 / UPEC) GN=thrS PE=3 SV=1 SYT_ECO81 - Threonine--tRNA ligase OS=Escherichia coli O81 (strain ED1a) GN=thrS PE=3 SV=1 gi\|254288567, gi... - gi\|254288567\|ref\|YP_003054315.1\| threonyl-tRNA synthetase [Escherichia coli BL21(DE3)], gi\|253977874... SYT_ECOL5 - Threonine--tRNA ligase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=thrS PE=3 SV=1 gi\|237705668, gi... - gi\|237705668\|ref\|ZP_04536149.1\| threonyl-tRNA synthetase [Escherichia sp. 3_2_53FAA], gi\|226900425\|g... EG11001 - threonyl-tRNA synthetase / Threonine--tRNA ligase, autogenously regulated; binds Zn(II) SYT_SHIF8 - Threonine--tRNA ligase OS=Shigella flexneri serotype 5b (strain 8401) GN=thrS PE=3 SV=1 SYT_ECOLU - Threonine--tRNA ligase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=thrS PE=3 SV=1 SYT_ECOBW - Threonine--tRNA ligase OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=thrS PE=3 SV=1 gi\|194422590, gi... - gi\|194438580\|ref\|ZP_03070669.1\| threonyl-tRNA synthetase [Escherichia coli 101-1], gi\|194422590\|gb\|E... gi\|194416606, gi... - gi\|194435142\|ref\|ZP_03067376.1\| threonyl-tRNA synthetase [Shigella dysenteriae 1012], gi\|194416606\|g... SYT_ECO45 - Threonine--tRNA ligase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=thrS PE=3 SV=1 gi\|254161779, gi... - gi\|254161779\|ref\|YP_003044887.1\| threonyl-tRNA synthetase [Escherichia coli B str. REL606], gi\|25397... SYT_ECO27 - Threonine--tRNA ligase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=thrS PE=3 SV=1	0.0	[3..399]	[208..597]
gi\|187776029, gi... - gi\|187776029\|ref\|ZP_02799987.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4196], gi\|... gi\|188016225, gi... - gi\|188024843\|ref\|ZP_02774486.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4113], gi\|... gi\|189010441, gi... - gi\|189010441\|ref\|ZP_02807141.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4076], gi\|... gi\|189356196, gi... - gi\|189402338\|ref\|ZP_02781727.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4401], gi\|... gi\|189367509, gi... - gi\|189367509\|gb\|EDU85925.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4501], gi\|1687... gi\|189372060, gi... - gi\|189372060\|gb\|EDU90476.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC869], gi\|16878... gi\|189377572, gi... - gi\|189377572\|gb\|EDU95988.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC508], gi\|16880... gi\|15831680, gi\|... - gi\|15831680\|ref\|NP_310453.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. Sakai], gi\|1336... gi\|254793336, gi... - gi\|254793336\|ref\|YP_003078173.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. TW14359], g... SYT_ECO57 - Threonine--tRNA ligase OS=Escherichia coli O157:H7 GN=thrS PE=3 SV=1 gi\|209768834, gi... - pir\|\|F85780 threonine tRNA synthetase [imported] - Escherichia coli (strain O157:H7, substrain EDL9... gi\|15802131, gi\|... - gi\|15802131\|ref\|NP_288153.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 EDL933], gi\|12515733... gi\|217318337, gi... - gi\|217328990\|ref\|ZP_03445071.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. TW14588], gi... gi\|189360618, gi... - gi\|189403439\|ref\|ZP_02795487.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4486], gi\|... gi\|208725260, gi... - gi\|208810744\|ref\|ZP_03252620.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4206], gi\|... gi\|208730990, gi... - gi\|208816647\|ref\|ZP_03257767.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4045], gi\|... SYT_ECO5E - Threonine--tRNA ligase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=thrS PE=3 SV=1	0.0	[3..399]	[208..597]

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Predicted Domain #1

Region A:
Residues: [1-421]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG  60
   61 TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG 120
  121 GELCSLRYDL TVPFARYVAM NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT 180
  181 FESMVPDSEC LSILVEGLTS LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL 240
  241 DKSPWEAVKK EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD 300
  301 DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE 360
  361 DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT 420
  421 I

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	285.228787
Match:	1kmmA_
Description:	Histidyl-tRNA synthetase (HisRS), C-terminal domain; Histidyl-tRNA synthetase (HisRS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.89468709683036	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.89468709683036	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.88410036819636	bayes_pls_golite062009
ligase activity	6.92073480193262	bayes_pls_golite062009
histidine-tRNA ligase activity	5.91343220471745	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	2.99105702052936	bayes_pls_golite062009
serine-tRNA ligase activity	2.46505535069103	bayes_pls_golite062009
glycine-tRNA ligase activity	2.29229136935761	bayes_pls_golite062009
threonine-tRNA ligase activity	2.18642354927	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
alanine-tRNA ligase activity	1.82594054955281	bayes_pls_golite062009
lysine-tRNA ligase activity	1.72324031407565	bayes_pls_golite062009
asparagine-tRNA ligase activity	0.921492123574877	bayes_pls_golite062009
binding	0.916222310895772	bayes_pls_golite062009
transcription regulator activity	0.375542987753806	bayes_pls_golite062009
nucleic acid binding	0.281662799812287	bayes_pls_golite062009
DNA binding	0.0309732052586011	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.00572514744734753	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [422-546]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KPTATQVFVM AFGGGKDWTG YLPERMKVTK QLWDAGIEAE YVYKAKANPR KQFDAAEKAG  60
   61 CHIAVILGKE EYLEGKLRVK RLGQEFADDD GELVSAADIV PIVQEKLSQI HEDGLNEVTR 120
  121 LIKGL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	285.228787
Match:	1kmmA_
Description:	Histidyl-tRNA synthetase (HisRS), C-terminal domain; Histidyl-tRNA synthetase (HisRS)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: