YRC Public Data Repository - Protein Structure Prediction

Protein:	THI80
Organism:	Saccharomyces cerevisiae
Length:	319 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THI80.

Description	E-value	Query Range	Subject Range
THI80_YEAST - Thiamine pyrophosphokinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI80 PE=1 SV... THI80 - Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (...	0.0	[1..319]	[1..319]
gi\|83755026, gi\|... - gi\|83755027\|pdb\|2F17\|B Chain B, Mouse Thiamin Pyrophosphokinase In A Ternary Complex With Pyrithiami...	5.0E-82	[29..310]	[29..261]
TPK1 - thiamin pyrophosphokinase 1	3.0E-77	[29..310]	[9..241]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	8.0E-63	[31..310]	[11..251]
gi\|18406621, gi\|... - gi\|20197027\|gb\|AAC27477.2\| putative thiamin pyrophosphokinase [Arabidopsis thaliana], gi\|20148329\|gb...	1.0E-62	[31..310]	[15..253]
TNR3_SCHPO - Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tnr3 PE=3 SV=1 tnr3 - thiamine diphosphokinase Tnr3	2.0E-58	[36..309]	[350..566]

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Predicted Domain #1

Region A:
Residues: [1-26]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEECIENPE RIKIGTDLIN IRNKMN

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [228-308]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FLIKKNGTLI EYDPQFRNTC IGNCGLLPIG EATLVKETRG LKWDVKNWPT SVVTGRVSSS  60
   61 NRFVGDNCCF IDTKDDIILN V

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10109.0
Match:	1ig0A_
Description:	Thiamin pyrophosphokinase, substrate-binding domain; Thiamin pyrophosphokinase, catalytic domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
thiamin diphosphokinase activity	3.17820968990148	bayes_pls_golite062009
catalytic activity	0.902495165806215	bayes_pls_golite062009
diphosphotransferase activity	0.763669021538822	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.0461369092625173	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [27-227]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LKELIHPNED ENSTLLILNQ KIDIPRPLFY KIWKLHDLKV CADGAANRLY DYLDDDETLR  60
   61 IKYLPNYIIG DLDSLSEKVY KYYRKNKVTI IKQTTQYSTD FTKCVNLISL HFNSPEFRSL 120
  121 ISNKDNLQSN HGIELEKGIH TLYNTMTESL VFSKVTPISL LALGGIGGRF DQTVHSITQL 180
  181 YTLSENASYF KLCYMTPTDL I

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [309-319]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EIFVDKLIDF L

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10109.0
Match:	1ig0A_
Description:	Thiamin pyrophosphokinase, substrate-binding domain; Thiamin pyrophosphokinase, catalytic domain
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: