YRC Public Data Repository - Protein Structure Prediction

Protein:	MAD2
Organism:	Saccharomyces cerevisiae
Length:	196 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MAD2.

Description	E-value	Query Range	Subject Range
MD2L1_MOUSE - Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus GN=Mad2l1 PE=1 SV=2	4.0E-76	[3..195]	[9..200]
gi\|190689935, gi... - gi\|190691307\|gb\|ACE87428.1\| MAD2 mitotic arrest deficient-like 1 (yeast) protein [synthetic construc... gi\|67969313 - gi\|67969313\|dbj\|BAE01009.1\| unnamed protein product [Macaca fascicularis] MAD2L1 - MAD2 mitotic arrest deficient-like 1 (yeast) ENSCJAT00000006092 - null ENSPTRT00000030501 - null ENSMMUT000000068... - null, null	3.0E-75	[3..195]	[9..200]
gi\|28175279, gi\|... - gi\|28175279\|gb\|AAH45227.1\| Mad2l1 protein [Xenopus laevis], gi\|1806617\|gb\|AAB41527.1\| spindle assemb...	1.0E-74	[3..195]	[8..198]
MAD2 - Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells wi... gi\|151945054 - gi\|151945054\|gb\|EDN63305.1\| spindle checkpoint complex subunit [Saccharomyces cerevisiae YJM789] gi\|207343944 - gi\|207343944\|gb\|EDZ71245.1\| YJL030Wp-like protein [Saccharomyces cerevisiae AWRI1631] gi\|190409468 - gi\|190409468\|gb\|EDV12733.1\| spindle checkpoint complex subunit [Saccharomyces cerevisiae RM11-1a] MAD2_YEAST - Mitotic spindle checkpoint component MAD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) G...	1.0E-71	[1..196]	[1..196]
MAD2_MAIZE - Mitotic spindle checkpoint protein MAD2 OS=Zea mays GN=MAD2 PE=2 SV=1	5.0E-69	[5..195]	[11..200]
MAD2_ARATH - Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana GN=MAD2 PE=1 SV=1	8.0E-69	[5..195]	[12..201]
mad2-PA - The gene mad2 is referred to in FlyBase by the symbol Dmel\mad2 (CG17498, FBgn0035640). It is a prot...	3.0E-67	[5..195]	[11..204]
MAD2_SCHPO - Mitotic spindle checkpoint component mad2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=... mad2 - spindle checkpoint protein Mad2	1.0E-66	[4..195]	[9..200]

Back

Predicted Domain #1

Region A:
Residues: [1-196]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR  60
   61 KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ 120
  121 IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT 180
  181 FSTNDHKVGA QVSYKY

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	448.232149
Match:	1klqA_
Description:	The spindle assembly checkpoint protein mad2
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
binding	2.14424444268625	bayes_pls_golite062009
protein binding	0.856075580719879	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: