YRC Public Data Repository - Protein Structure Prediction

Protein:	TWF1
Organism:	Saccharomyces cerevisiae
Length:	332 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TWF1.

Description	E-value	Query Range	Subject Range
gi\|207345159 - gi\|207345159\|gb\|EDZ72069.1\| YGR080Wp-like protein [Saccharomyces cerevisiae AWRI1631] gi\|190406900 - gi\|190406900\|gb\|EDV10167.1\| twinfilin A [Saccharomyces cerevisiae RM11-1a] TWF1_YEAST - Twinfilin-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TWF1 PE=1 SV=1 TWF1 - Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortica...	4.0E-92	[1..332]	[1..332]
TWF1_MOUSE - (Q91YR1) Twinfilin-1 (A6 protein) (Protein tyrosine kinase 9)	9.0E-85	[1..330]	[1..333]
TWF2 - twinfilin, actin-binding protein, homolog 2 (Drosophila) gi\|123981702, gi... - gi\|168278421\|dbj\|BAG11090.1\| twinfilin-2 [synthetic construct], gi\|123996521\|gb\|ABM85862.1\| PTK9L pr...	8.0E-80	[1..330]	[1..333]
gi\|194900685, gi... - gi\|194900685\|ref\|XP_001979886.1\| GG16839 [Drosophila erecta], gi\|190651589\|gb\|EDV48844.1\| GG16839 [D... gi\|220956446 - gi\|220956446\|gb\|ACL90766.1\| twf-PA [synthetic construct] twf-PA - The gene twinfilin is referred to in FlyBase by the symbol Dmel\twf (CG3172, FBgn0038206). It is a p...	6.0E-73	[1..332]	[1..337]
CE28307 - protein tyrosine kinase status:Confirmed UniProt:Q20173 protein_id:AAA93345.2	7.0E-70	[1..331]	[1..329]
TWF1_SCHPO - Twinfilin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=twf1 PE=1 SV=1 twf1 - twinfilin	4.0E-60	[1..296]	[1..300]

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Predicted Domain #1

Region A:
Residues: [1-166]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE ELVQLASQER  60
   61 EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL ARQVGSNSLS TEQPLITDAQ 120
  121 DLVDLKNFDS ARPAGQNKPL THDEEMQIEI NKQQALLRKN TSVKLV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	44.39794
Match:	1f7sA_
Description:	Cofilin (actin depolymerizing factor, ADF)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
actin binding	7.52071741004022	bayes_pls_golite062009
cytoskeletal protein binding	6.04859625643932	bayes_pls_golite062009
actin filament binding	5.45532510538573	bayes_pls_golite062009
binding	2.41877859618486	bayes_pls_golite062009
protein kinase activity	2.22634235673758	bayes_pls_golite062009
protein binding	1.87981807141519	bayes_pls_golite062009
protein tyrosine kinase activity	1.87091423399482	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.67227267699496	bayes_pls_golite062009
kinase activity	1.55883505515498	bayes_pls_golite062009
phosphatidylinositol-4,5-bisphosphate binding	1.3406661967545	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.30213296029859	bayes_pls_golite062009
transferase activity	0.591259446255369	bayes_pls_golite062009
structural constituent of cytoskeleton	0.482954032096802	bayes_pls_golite062009
structural molecule activity	0.391701411061931	bayes_pls_golite062009
molecular transducer activity	0.293715974535634	bayes_pls_golite062009
signal transducer activity	0.293715974535634	bayes_pls_golite062009
actin monomer binding	0.224304003054399	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [167-332]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SQDSASPLSL TFRVNSEKPI NEILDSEGKN LIIFQIDPSN ETIQIVQSDT CPSVDELYID  60
   61 LPGPSYTIFR QGDSSFFIYS CPSGSKVKDR MIYASNKNGF INYLKNDQKI AFSKVVEIGD 120
  121 FVELDKSLLM ATNKEDSLDH GSNPDLPNKS NLKFNKPKGP LRKRRT

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	14.09
Match:	1cfyA
Description:	Cofilin (actin depolymerizing factor, ADF)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: