YRC Public Data Repository - Protein Structure Prediction

Protein:	PUS2
Organism:	Saccharomyces cerevisiae
Length:	370 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUS2.

Description	E-value	Query Range	Subject Range
PUS2 - Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; effi... gi\|6321375\|ref\|N... - Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; effi...	0.0	[1..370]	[1..370]
gi\|23308411, gi\|... - gi\|6573728\|gb\|AAF17648.1\|AC009978_24 T23E18.5 [Arabidopsis thaliana], gi\|23308411\|gb\|AAN18175.1\| At1...	2.0E-85	[1..368]	[26..435]
PUS2_SCHPO - tRNA pseudouridine synthase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pus2 PE=3 SV... pus2 - tRNA pseudouridylate synthase Pus2	1.0E-75	[1..364]	[43..440]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	6.0E-67	[2..346]	[5..330]
gi\|293415611\|ref... - tRNA pseudouridine synthase A [Escherichia coli B185] TRUA_ECOL6 - tRNA pseudouridine synthase A OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=truA... gi\|209764792, gi... - pir\|\|C85873 pseudouridylate synthase I [imported] - Escherichia coli (strain O157:H7, substrain EDL... gi\|291283559\|ref... - tRNA pseudouridine synthase A [Escherichia coli O55:H7 str. CB9615] gi\|12516677, gi\|... - gi\|15802865\|ref\|NP_288892.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 EDL933], gi\|125... gi\|189362830, gi... - gi\|189362830\|gb\|EDU81249.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4486], gi... TRUA_ECO57 - tRNA pseudouridine synthase A OS=Escherichia coli O157:H7 GN=truA PE=3 SV=1 gi\|168761239, gi... - gi\|189368293\|gb\|EDU86709.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4501], gi... TRUA_ECOL6 - tRNA pseudouridine synthase A OS=Escherichia coli O6 GN=truA PE=3 SV=1 gi\|189357607, gi... - gi\|189357607\|gb\|EDU76026.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4401], gi... gi\|293410675\|ref... - tRNA pseudouridine synthase A [Escherichia coli B354] gi\|261223227\|ref... - tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. FRIK2000] gi\|188017261, gi... - gi\|188017261\|gb\|EDU55383.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4113], gi... gi\|168798493, gi... - gi\|189378874\|gb\|EDU97290.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC508], gi\|... gi\|168787230, gi... - gi\|189372810\|gb\|EDU91226.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC869], gi\|... gi\|168780178, gi... - gi\|189001906\|gb\|EDU70892.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4076], gi... gi\|187771144, gi... - gi\|187771144\|gb\|EDU34988.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4196], gi... gi\|293405784\|ref... - tRNA pseudouridine synthase A [Escherichia coli FVEC1412] gi\|13362672, gi\|... - gi\|15832456\|ref\|NP_311229.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. Sakai], gi... gi\|261259223\|ref... - tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. FRIK966] TRUA_ECOLU - tRNA pseudouridine synthase A OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=truA PE=3 S... TRUA_ECO7I - tRNA pseudouridine synthase A OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=truA PE=3 SV=1 gi\|208811862, gi... - gi\|208811862\|ref\|ZP_03253191.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4045]... gi\|208727696, gi... - gi\|208807895\|ref\|ZP_03250232.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4206]... gi\|195935697 - gi\|195935697\|ref\|ZP_03081079.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4024] TRUA_ECOSM - tRNA pseudouridine synthase A OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=truA PE=3 SV=1 TRUA_ECO55 - tRNA pseudouridine synthase A OS=Escherichia coli (strain 55989 / EAEC) GN=truA PE=3 SV=1 gi\|217319539, gi... - gi\|217327172\|ref\|ZP_03443255.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. TW14588... TRUA_ECO5E - tRNA pseudouridine synthase A OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=truA PE=3 SV=1 gi\|208741667, gi... - gi\|208821544\|ref\|ZP_03261864.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. EC4042]... gi\|293446655\|ref... - tRNA pseudouridine synthase A [Escherichia coli B088] gi\|254794211, gi... - gi\|254794211\|ref\|YP_003079048.1\| tRNA pseudouridine synthase A [Escherichia coli O157:H7 str. TW1435... gi\|227887378, gi... - gi\|227887378\|ref\|ZP_04005183.1\| tRNA pseudouridine synthase A [Escherichia coli 83972], gi\|227835728... TRUA_SHIDS - tRNA pseudouridine synthase A OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=truA PE=3 SV=1	3.0E-59	[1..215]	[14..218]

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Predicted Domain #1

Region A:
Residues: [1-124]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM AAARTDKGVH  60
   61 AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF NARSACDSRW YQYLIPEFIL 120
  121 IGPP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	109.980454
Match:	1dj0A_
Description:	Pseudouridine synthase I
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
pseudouridine synthase activity	5.19957135839682	bayes_pls_golite062009
intramolecular transferase activity	3.78688422405974	bayes_pls_golite062009
isomerase activity	2.91720316078381	bayes_pls_golite062009
transcription regulator activity	2.31595692797262	bayes_pls_golite062009
catalytic activity	2.02180970970004	bayes_pls_golite062009
nucleic acid binding	2.01176877679953	bayes_pls_golite062009
DNA binding	1.95629441517514	bayes_pls_golite062009
binding	1.32358464424761	bayes_pls_golite062009
transcription activator activity	1.27357280497723	bayes_pls_golite062009
protein binding	1.10318302423731	bayes_pls_golite062009
RNA binding	0.706879359688019	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [125-215]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RSSLLHRNVG GSYREDGSQE VWDTFLEQTR GRFSGDELCR LQDTAQKLSE SDPLVQDYVG  60
   61 LLSGTLSGYC LSPSKLDAFE AAMQEYVGTH N

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	109.980454
Match:	1dj0A_
Description:	Pseudouridine synthase I
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [216-370]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FHNFTTGKLW GDPSAQRHIK KVVVSQASPG WICVRIHGQS FMLHQIRRMV ALAVLAARCQ  60
   61 LPPNIVRNYF NAGPRKYIPR APAQGLLLEG PVFDGYNTKL RNLLYCEIRP DDITLERMCR 120
  121 FRERQICTAI AHEETQRHVF CHFVRQMNRL ATPLI

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	7.920819
Match:	PF01416
Description:	tRNA pseudouridine synthase

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: