YRC Public Data Repository - Protein Structure Prediction

Protein:	GCST_ECOLI
Organism:	Escherichia coli
Length:	364 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GCST_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|75239248 - gi\|75239248\|ref\|ZP_00723221.1\| COG0404: Glycine cleavage system T protein (aminomethyltransferase) [... gi\|117625135, gi... - gi\|117625135\|ref\|YP_854123.1\| glycine cleavage system aminomethyltransferase T [Escherichia coli APE... gi\|110344645, gi... - gi\|110643053\|ref\|YP_670783.1\| glycine cleavage system aminomethyltransferase T [Escherichia coli 536...	404.0	[0..1]	[364..24]
GCST_SHIFL - Aminomethyltransferase OS=Shigella flexneri GN=gcvT PE=3 SV=1 gi\|24053299, gi\|... - gi\|24114158\|ref\|NP_708668.1\| glycine cleavage system aminomethyltransferase T [Shigella flexneri 2a ... gi\|30042473, gi\|... - gi\|30064216\|ref\|NP_838387.1\| glycine cleavage system aminomethyltransferase T [Shigella flexneri 2a ...	402.0	[0..1]	[364..1]
sp\|B7UHV3\|GCST_ECO27 - Aminomethyltransferase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=gcvT PE=3 SV=1 gi\|190906457, gi... - gi\|191173206\|ref\|ZP_03034737.1\| glycine cleavage system T protein [Escherichia coli F11], gi\|1909064... GCST_ECO27 - Aminomethyltransferase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=gcvT PE=3 SV=1 GCST_ESCF3 - Aminomethyltransferase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=gc... GCST_ECO45 - Aminomethyltransferase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=gcvT PE=3 SV=1 GCST_ECO7I - Aminomethyltransferase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=gcvT PE=3 SV=1 gi\|229560214 - gi\|229560214\|ref\|YP_670783.2\| glycine cleavage system aminomethyltransferase T [Escherichia coli 536... gi\|229775962 - gi\|229775962\|ref\|YP_854123.2\| glycine cleavage system aminomethyltransferase T [Escherichia coli APE... GCST_ECOUT - Aminomethyltransferase OS=Escherichia coli (strain UTI89 / UPEC) GN=gcvT PE=3 SV=1 gi\|237706451, gi... - gi\|237706451\|ref\|ZP_04536932.1\| glycine cleavage system aminomethyltransferase T [Escherichia sp. 3_... gi\|226839573, gi... - gi\|254037949\|ref\|ZP_04872007.1\| glycine cleavage system aminomethyltransferase T [Escherichia sp. 1_...	401.0	[0..1]	[364..1]
GCST_SHIDS - Aminomethyltransferase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=gcvT PE=3 SV=1	401.0	[0..1]	[364..1]
GCST_ECOL6 - Aminomethyltransferase OS=Escherichia coli O6 GN=gcvT PE=3 SV=1 GCST_ECOL6 - Aminomethyltransferase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=gcvT PE=3 S... gi\|227834723, gi... - gi\|227888454\|ref\|ZP_04006259.1\| glycine cleavage system aminomethyltransferase T [Escherichia coli 8...	401.0	[0..1]	[364..1]
GCST_YERPS - Aminomethyltransferase OS=Yersinia pseudotuberculosis serotype I (strain IP32953) GN=gcvT PE=3 SV=1 GCST_YERPS - Aminomethyltransferase OS=Yersinia pseudotuberculosis GN=gcvT PE=3 SV=1 GCST_YERP3 - Aminomethyltransferase OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=gcvT PE=3 S...	400.0	[0..1]	[363..1]
GCST_SHIB3 - Aminomethyltransferase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=gcvT PE=3 SV=1	400.0	[0..1]	[364..1]
gi\|149289490, gi... - gi\|150260082\|ref\|ZP_01916810.1\| aminomethyltransferase [Yersinia pestis CA88-4125], gi\|149289490\|gb\|... GCST_YERPP - Aminomethyltransferase OS=Yersinia pestis (strain Pestoides F) GN=gcvT PE=3 SV=1 GCST_YERPE - Aminomethyltransferase OS=Yersinia pestis GN=gcvT PE=3 SV=1 gi\|21960233, gi\|... - gi\|22127170\|ref\|NP_670593.1\| glycine cleavage system aminomethyltransferase T [Yersinia pestis KIM],... gi\|115346691, gi... - gi\|218928080\|ref\|YP_002345955.1\| glycine cleavage system aminomethyltransferase T [Yersinia pestis C... gi\|45443338, gi\|... - gi\|45443338\|ref\|NP_994877.1\| glycine cleavage system aminomethyltransferase T [Yersinia pestis biova... gi\|229837595, gi... - gi\|229837595\|ref\|ZP_04457757.1\| aminomethyltransferase, tetrahydrofolate-dependent, subunit (T prote... gi\|229840819, gi... - gi\|229840819\|ref\|ZP_04460978.1\| aminomethyltransferase, tetrahydrofolate-dependent, subunit (T prote... gi\|229842622, gi... - gi\|229842622\|ref\|ZP_04462777.1\| aminomethyltransferase, tetrahydrofolate-dependent, subunit (T prote... GCST_YERPB - Aminomethyltransferase OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=gcvT PE=3 SV=1 gi\|165914681, gi... - gi\|89102642\|ref\|ZP_01175260.1\| COG0404: Glycine cleavage system T protein (aminomethyltransferase) [... gi\|165923172, gi... - gi\|165924972\|ref\|ZP_02220804.1\| glycine cleavage system T protein [Yersinia pestis biovar Orientalis... GCST_YERPA - Aminomethyltransferase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=gcvT PE=3 SV=1 GCST_YERPN - Aminomethyltransferase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=gcvT PE=3 SV=1 gi\|166205998, gi... - gi\|166212706\|ref\|ZP_02238741.1\| glycine cleavage system T protein [Yersinia pestis biovar Antiqua st... gi\|165990820, gi... - gi\|166010334\|ref\|ZP_02231232.1\| glycine cleavage system T protein [Yersinia pestis biovar Antiqua st... gi\|167419345, gi... - gi\|167419345\|ref\|ZP_02311098.1\| glycine cleavage system T protein [Yersinia pestis biovar Orientalis... gi\|167399824, gi... - gi\|167399824\|ref\|ZP_02305342.1\| glycine cleavage system T protein [Yersinia pestis biovar Antiqua st... gi\|167467675 - gi\|167467675\|ref\|ZP_02332379.1\| glycine cleavage system T protein [Yersinia pestis FV-1] GCST_YERPG - Aminomethyltransferase OS=Yersinia pestis bv. Antiqua (strain Angola) GN=gcvT PE=3 SV=1 gi\|167055699, gi... - gi\|167425299\|ref\|ZP_02317052.1\| glycine cleavage system T protein [Yersinia pestis biovar Mediaevali...	400.0	[0..1]	[363..1]
gi\|75515312, gi\|... - gi\|75515312\|ref\|ZP_00737496.1\| COG0404: Glycine cleavage system T protein (aminomethyltransferase) [...	400.0	[0..1]	[364..1]
GCST_SHESM - Aminomethyltransferase OS=Shewanella sp. (strain MR-4) GN=gcvT PE=3 SV=1	399.0	[0..1]	[361..1]

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Predicted Domain #1

Region A:
Residues: [1-364]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAQQTPLYEQ HTLCGARMVD FHGWMMPLHY GSQIDEHHAV RTDAGMFDVS HMTIVDLRGS  60
   61 RTREFLRYLL ANDVAKLTKS GKALYSGMLN ASGGVIDDLI VYYFTEDFFR LVVNSATREK 120
  121 DLSWITQHAE PFGIEITVRD DLSMIAVQGP NAQAKAATLF NDAQRQAVEG MKPFFGVQAG 180
  181 DLFIATTGYT GEAGYEIALP NEKAADFWRA LVEAGVKPCG LGARDTLRLE AGMNLYGQEM 240
  241 DETISPLAAN MGWTIAWEPA DRDFIGREAL EVQREHGTEK LVGLVMTEKG VLRNELPVRF 300
  301 TDAQGNQHEG IITSGTFSPT LGYSIALARV PEGIGETAIV QIRNREMPVK VTKPVFVRNG 360
  361 KAVA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	111.0
Match:	1vloA
Description:	Crystal structure of aminomethyltransferase (T protein; tetrahydrofolate-dependent) of glycine cleavage system (np417381) from Escherichia coli k12 at 1.70 A resolution
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
aminomethyltransferase activity	10.5654741349554	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	5.37200252580633	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	5.37200252580633	bayes_pls_golite062009
methyltransferase activity	3.28983380541263	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	3.20782575104451	bayes_pls_golite062009
catalytic activity	1.65079399897053	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	1.19783690850993	bayes_pls_golite062009
oxidoreductase activity	0.867567984371732	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH group of donors	0.550291785460228	bayes_pls_golite062009
transferase activity	0.217873555384951	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: