YRC Public Data Repository - Protein Structure Prediction

Protein:	PUF3_YEAST
Organism:	Saccharomyces cerevisiae S288c
Length:	879 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUF3_YEAST.

Description	E-value	Query Range	Subject Range
PUF3 - Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anter... PUF3_YEAST - mRNA-binding protein PUF3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUF3 PE=1 SV=1	0.0	[1..879]	[1..879]
PUM1_ARATH - Pumilio homolog 1 OS=Arabidopsis thaliana GN=APUM1 PE=1 SV=1	0.0	[85..875]	[184..954]
gi\|24159015, gi\|... - gi\|24159016\|pdb\|1M8Y\|B Chain B, Crystal Structure Of The Pumilio-Homology Domain From Human Pumilio1...	0.0	[514..875]	[2..345]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[79..879]	[23..847]
gi\|14719326 - gi\|14719326\|gb\|AAK73144.1\|AC079022_17 putative pumilio/Mpt5 family RNA-binding protein [Oryza sativa... gi\|50949037 - gi\|50949037\|ref\|XP_493876.1\| putative pumilio/Mpt5 family RNA-binding protein [Oryza sativa]	0.0	[44..875]	[88..838]
gi\|8071634 - gi\|8071634\|gb\|AAF71823.1\|AF153276_1 pumilio domain-containing protein PPD1 [Populus tremula x Populu...	0.0	[49..852]	[156..966]

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Predicted Domain #1

Region A:
Residues: [1-147]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEMNMDMDMD MELASIVSSL SALSHSNNNG GQAAAAGIVN GGAAGSQQIG GFRRSSFTTA  60
   61 NEVDSEILLL HGSSESSPIF KKTALSVGTA PPFSTNSKKF FGNGGNYYQY RSTDTASLSS 120
  121 ASYNNYHTHH TAANLGKNNK VNHLLGQ

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [148-513]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YSASIAGPVY YNGNDNNNSG GEGFFEKFGK SLIDGTRELE SQDRPDAVNT QSQFISKSVS  60
   61 NASLDTQNTF EQNVESDKNF NKLNRNTTNS GSLYHSSSNS GSSASLESEN AHYPKRNIWN 120
  121 VANTPVFRPS NNPAAVGATN VALPNQQDGP ANNNFPPYMN GFPPNQFHQG PHYQNFPNYL 180
  181 IGSPSNFISQ MISVQIPANE DTEDSNGKKK KKANRPSSVS SPSSPPNNSP FPFAYPNPMM 240
  241 FMPPPPLSAP QQQQQQQQQQ QQEDQQQQQQ QENPYIYYPT PNPIPVKMPK DEKTFKKRNN 300
  301 KNHPANNSNN ANKQANPYLE NSIPTKNTSK KNASSKSNES TANNHKSHSH SHPHSQSLQQ 360
  361 QQQTYH

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	14.59
Match:	1k83A
Description:	RBP1
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [514-647]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RSPLLEQLRN SSSDKNSNSN MSLKDIFGHS LEFCKDQHGS RFIQRELATS PASEKEVIFN  60
   61 EIRDDAIELS NDVFGNYVIQ KFFEFGSKIQ KNTLVDQFKG NMKQLSLQMY ACRVIQKALE 120
  121 YIDSNQRIEL VLEL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	973.9794
Match:	1ib2A_
Description:	Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
protein transporter activity	3.78867875686001	bayes_pls_golite062009
binding	2.48958374349477	bayes_pls_golite062009
protein transmembrane transporter activity	2.31781486383479	bayes_pls_golite062009
RNA binding	2.11573661150436	bayes_pls_golite062009
transporter activity	2.07943941884833	bayes_pls_golite062009
substrate-specific transporter activity	1.94695201254013	bayes_pls_golite062009
protein binding	1.94427330399472	bayes_pls_golite062009
nucleic acid binding	1.25530318108311	bayes_pls_golite062009
transmembrane transporter activity	0.912298340413274	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.798034501840742	bayes_pls_golite062009
mRNA binding	0.73831124805124	bayes_pls_golite062009
transcription regulator activity	0.464230801825493	bayes_pls_golite062009
DNA binding	0.356030945125909	bayes_pls_golite062009
hydrolase activity	0.251693850515235	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.184228011150913	bayes_pls_golite062009
signal sequence binding	0.14931345687137	bayes_pls_golite062009
nuclear localization sequence binding	0.144906281351729	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.128082902706083	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [648-746]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDSVLQMIKD QNGNHVIQKA IETIPIEKLP FILSSLTGHI YHLSTHSYGC RVIQRLLEFG  60
   61 SSEDQESILN ELKDFIPYLI QDQYGNYVIQ YVLQQDQFT

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	973.9794
Match:	1ib2A_
Description:	Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [747-879]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NKEMVDIKQE IIETVANNVV EYSKHKFASN VVEKSILYGS KNQKDLIISK ILPRDKNHAL  60
   61 NLEDDSPMIL MIKDQFANYV IQKLVNVSEG EGKKLIVIAI RAYLDKLNKS NSLGNRHLAS 120
  121 VEKLAALVEN AEV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	973.9794
Match:	1ib2A_
Description:	Pumilio 1
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: