Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 16 | 20 | 92.8% | 0.238 | 7729 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 187 | 237 | 66.8% | 0.116 | 195140 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 13 | 15 | 61.5% | 0.0765 | 18751 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 29 | 32 | 61.4% | 0.0724 | 42271 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 72 | 81 | 51.4% | 0.0705 | 111782 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 24 | 24 | 48.2% | 0.0339 | 67400 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 13 | 15 | 47.4% | 0.0487 | 29280 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 4 | 4 | 44.9% | 0.0223 | 16135 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 9 | 9 | 39.5% | 0.0156 | 52628 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 10 | 10 | 31.2% | 0.0213 | 45384 |
[ |
TCP1 | Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... | 5 | 5 | 23.8% | 0.00735 | 60481 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 6 | 6 | 21.6% | 0.0154 | 37354 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 4 | 4 | 21.2% | 0.012 | 29995 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 10 | 22 | 20.2% | 0.022 | 96825 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 7 | 7 | 18.5% | 0.01 | 66087 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 5 | 5 | 16.4% | 0.00948 | 50653 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 4 | 4 | 13.7% | 0.00554 | 66953 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 5 | 5 | 13.4% | 0.0106 | 44585 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 8 | 8 | 12.6% | 0.00772 | 94104 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 5 | 11 | 12.3% | 0.0107 | 98227 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 8 | 9 | 10.5% | 0.00805 | 99619 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 8 | 8 | 10.5% | 0.00794 | 95153 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 4 | 4 | 9.3% | 0.0039 | 93289 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 4 | 4 | 8.7% | 0.00482 | 74468 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 8 | 8 | 6.2% | 0.0035 | 218454 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 2 | 2 | 3.9% | 0.00108 | 175459 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 2 | 2 | 3.7% | 0.00264 | 72105 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 2 | 2 | 2.7% | 0.00123 | 151652 |