YRC Public Data Repository - Protein Structure Prediction

Protein:	MENE_ECOLI
Organism:	Escherichia coli
Length:	451 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MENE_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|75701558, gi\|... - gi\|75907833\|ref\|YP_322129.1\| amino acid adenylation [Anabaena variabilis ATCC 29413], gi\|75701558\|gb...	458.0	[0..3]	[449..545]
gi\|49610951, gi\|... - gi\|50120425\|ref\|YP_049592.1\| non-ribosomal peptide synthetase [Pectobacterium atrosepticum SCRI1043]...	454.0	[0..3]	[448..2670]
gi\|89203970, gi\|... - gi\|89203970\|ref\|ZP_01182549.1\| Non-ribosomal peptide synthase:Amino acid adenylation [Bacillus weihe...	453.0	[0..3]	[449..492]
gi\|17131741, gi\|... - gi\|17230140\|ref\|NP_486688.1\| peptide synthetase [Nostoc sp. PCC 7120], gi\|17131741\|dbj\|BAB74347.1\| p... gi\|25294032 - pir\|\|AI2136 peptide synthetase [imported] - Nostoc sp. (strain PCC 7120)	453.0	[0..3]	[446..461]
gi\|27817222 - gi\|27817222\|gb\|AAO23333.1\| NcpA [Nostoc sp. ATCC 53789]	448.0	[0..3]	[449..2667]
LGRD_BREPA - Linear gramicidin synthase subunit D OS=Brevibacillus parabrevis GN=lgrD PE=1 SV=1 gi\|42820781 - gi\|42820781\|emb\|CAD92852.1\| nonribosomal peptide synthetase [Brevibacillus brevis]	442.0	[0..3]	[450..464]
gi\|186682529, gi... - gi\|23129063\|ref\|ZP_00110897.1\| COG1020: Non-ribosomal peptide synthetase modules and related protein...	442.0	[0..5]	[446..1572]

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Predicted Domain #1

Region A:
Residues: [1-451]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIFSDWPWRH WRQVRGETIA LRLNDEQLNW RELCARVDEL ASGFAVQGVV EGSGVMLRAW  60
   61 NTPQTLLAWL ALLQCGARVL PVNPQLPQPL LEELLPNLTL QFALVPDGEN TFPALTSLHI 120
  121 QLVEGAHAAT WQPTRLCSMT LTSGSTGLPK AAVHTYQAHL ASAQGVLSLI PFGDHDDWLL 180
  181 SLPLFHVSGQ GIMWRWLYAG ARMTVRDKQP LEQMLAGCTH ASLVPTQLWR LLVNRSSVSL 240
  241 KAVLLGGAAI PVELTEQARE QGIRCFCGYG LTEFASTVCA KEADGLADVG SPLPGREVKI 300
  301 VNNEVWLRAA SMAEGYWRNG QLVSLVNDEG WYATRDRGEM HNGKLTIVGR LDNLFFSGGE 360
  361 GIQPEEVERV IAAHPAVLQV FIVPVADKEF GHRPVAVMEY DHESVDLSEW VKDKLARFQQ 420
  421 PVRWLTLPPE LKNGGIKISR QALKEWVQRQ Q

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	110.0
Match:	1amuA
Description:	Phenylalanine activating domain of gramicidin synthetase 1
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming carbon-sulfur bonds	2.82174699142286	bayes_pls_golite062009
fatty acid ligase activity	2.47132758884786	bayes_pls_golite062009
long-chain fatty acid-CoA ligase activity	2.24834398174487	bayes_pls_golite062009
catalytic activity	2.20589179498934	bayes_pls_golite062009
ligase activity	2.04217449574493	bayes_pls_golite062009
acid-thiol ligase activity	2.03342160490677	bayes_pls_golite062009
CoA-ligase activity	1.74361558346113	bayes_pls_golite062009
acetate-CoA ligase activity	1.55149187194463	bayes_pls_golite062009
benzoate-CoA ligase activity	1.32376625973206	bayes_pls_golite062009
o-succinylbenzoate-CoA ligase activity	0.97784533685802	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: