YRC Public Data Repository - Protein Structure Prediction

Protein:	HOLB_ECOLI
Organism:	Escherichia coli
Length:	334 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HOLB_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|48862900, gi\|... - gi\|90020982\|ref\|YP_526809.1\| DNA polymerase III subunits gamma and tau [Saccharophagus degradans 2-4...	247.0	[0..2]	[332..9]
gi\|76793556, gi\|... - gi\|76793556\|ref\|ZP_00776041.1\| DNA-directed DNA polymerase [Pseudoalteromonas atlantica T6c], gi\|765...	247.0	[0..2]	[330..9]
gi\|114334870, gi... - gi\|114563577\|ref\|YP_751090.1\| DNA polymerase III, subunits gamma and tau [Shewanella frigidimarina N...	245.0	[0..2]	[326..9]
gi\|71734194, gi\|... - gi\|71734194\|ref\|YP_274019.1\| DNA polymerase III subunits gamma and tau [Pseudomonas syringae pv. pha...	241.0	[0..2]	[333..9]
gi\|89094005, gi\|... - gi\|89094005\|ref\|ZP_01166949.1\| DNA polymerase subunits gamma and tau [Oceanospirillum sp. MED92], gi...	241.0	[0..2]	[330..9]
gi\|77458032, gi\|... - gi\|77458032\|ref\|YP_347537.1\| DNA polymerase III subunits gamma and tau [Pseudomonas fluorescens Pf0-...	241.0	[0..2]	[333..9]
gi\|88794015, gi\|... - gi\|88794015\|ref\|ZP_01109726.1\| DNA polymerase III subunits gamma and tau [Alteromonas macleodii 'Dee...	240.0	[0..2]	[327..9]
gi\|83633436, gi\|... - gi\|83645393\|ref\|YP_433828.1\| DNA polymerase III subunits gamma and tau [Hahella chejuensis KCTC 2396...	240.0	[0..2]	[326..9]
gi\|82411044, gi\|... - gi\|82702979\|ref\|YP_412545.1\| DNA-directed DNA polymerase [Nitrosospira multiformis ATCC 25196], gi\|8...	240.0	[0..2]	[319..11]
gi\|113970589, gi... - gi\|82496658\|ref\|ZP_00882223.1\| ATPas [Shewanella sp. MR-4], gi\|82405233\|gb\|EAP45908.1\| ATPas [Shewan...	240.0	[0..2]	[318..9]

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Predicted Domain #1

Region A:
Residues: [1-334]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRWYPWLRPD FEKLVASYQA GRGHHALLIQ ALPGMGDDAL IYALSRYLLC QQPQGHKSCG  60
   61 HCRGCQLMQA GTHPDYYTLA PEKGKNTLGV DAVREVTEKL NEHARLGGAK VVWVTDAALL 120
  121 TDAAANALLK TLEEPPAETW FFLATREPER LLATLRSRCR LHYLAPPPEQ YAVTWLSREV 180
  181 TMSQDALLAA LRLSAGSPGA ALALFQGDNW QARETLCQAL AYSVPSGDWY SLLAALNHEQ 240
  241 APARLHWLAT LLMDALKRHH GAAQVTNVDV PGLVAELANH LSPSRLQAIL GDVCHIREQL 300
  301 MSVTGINREL LITDLLLRIE HYLQPGVVLP VPHL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	53.045757
Match:	1a5tA
Description:	CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
DNA-directed DNA polymerase activity	6.09279996112705	bayes_pls_golite062009
DNA polymerase activity	6.04865791514294	bayes_pls_golite062009
nucleotidyltransferase activity	3.99580940620614	bayes_pls_golite062009
pyrophosphatase activity	2.23646936731119	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.77451606086272	bayes_pls_golite062009
catalytic activity	1.32370671504792	bayes_pls_golite062009
transferase activity	1.23626141560528	bayes_pls_golite062009
helicase activity	0.895387263223335	bayes_pls_golite062009
DNA helicase activity	0.8104901979223	bayes_pls_golite062009
nucleic acid binding	0.625617128641089	bayes_pls_golite062009
binding	0.594355060369958	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.248432446853676	bayes_pls_golite062009
protein-DNA loading ATPase activity	0.24468171323128	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.217252531553173	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.215962398124297	bayes_pls_golite062009
DNA clamp loader activity	0.20238478387743	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.156322124775303	bayes_pls_golite062009
ATP-dependent helicase activity	0.156322124775303	bayes_pls_golite062009
ATPase activity	0.0740243885501273	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.0398638249772338	bayes_pls_golite062009
DNA binding	0.0064778111446141	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: