YRC Public Data Repository - Protein Structure Prediction

Protein:	GSHB_ECOLI
Organism:	Escherichia coli
Length:	316 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GSHB_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|77961493, gi\|... - gi\|77961493\|ref\|ZP_00825329.1\| COG0189: Glutathione synthase/Ribosomal protein S6 modification enzym...	285.0	[0..1]	[316..1]
GSHB_VIBVY - Glutathione synthetase OS=Vibrio vulnificus (strain YJ016) GN=gshB PE=3 SV=1	285.0	[0..1]	[316..2]
gi\|149367056, gi... - gi\|149367056\|ref\|ZP_01889089.1\| glutathione synthetase [Yersinia pestis CA88-4125], gi\|149290670\|gb\|... gi\|145597887, gi... - gi\|145597887\|ref\|YP_001161963.1\| glutathione synthetase [Yersinia pestis Pestoides F], gi\|145209583\|... gi\|165937233, gi... - gi\|89104338\|ref\|ZP_01176884.1\| COG0189: Glutathione synthase/Ribosomal protein S6 modification enzym... gi\|108778240, gi... - gi\|108806327\|ref\|YP_650243.1\| glutathione synthetase [Yersinia pestis Antiqua], gi\|108778240\|gb\|ABG1... gi\|108813292, gi... - gi\|229903755\|ref\|ZP_04518868.1\| glutathione synthetase [Yersinia pestis Nepal516], gi\|229679525\|gb\|E... gi\|186896640, gi... - gi\|186896640\|ref\|YP_001873752.1\| glutathione synthetase [Yersinia pseudotuberculosis PB1/+], gi\|1866... gi\|45438113, gi\|... - gi\|45443245\|ref\|NP_994784.1\| glutathione synthetase [Yersinia pestis biovar Microtus str. 91001], gi... gi\|22127197, gi\|... - gi\|22127197\|ref\|NP_670620.1\| glutathione synthetase [Yersinia pestis KIM], gi\|21960263\|gb\|AAM86871.1... gi\|229837627, gi... - gi\|229837627\|ref\|ZP_04457789.1\| glutathione synthetase [Yersinia pestis Pestoides A], gi\|229704315\|g... gi\|115346718, gi... - gi\|218928107\|ref\|YP_002345982.1\| glutathione synthetase [Yersinia pestis CO92], gi\|16121239\|ref\|NP_4... gi\|229840854, gi... - gi\|229840854\|ref\|ZP_04461013.1\| glutathione synthetase [Yersinia pestis biovar Orientalis str. PEXU2... gi\|51590799, gi\|... - gi\|51597517\|ref\|YP_071708.1\| glutathione synthetase [Yersinia pseudotuberculosis IP 32953], gi\|51590... gi\|229690896, gi... - gi\|229842586\|ref\|ZP_04462741.1\| glutathione synthetase [Yersinia pestis biovar Orientalis str. India... gi\|162418966, gi... - gi\|162418966\|ref\|YP_001607685.1\| glutathione synthetase [Yersinia pestis Angola], gi\|162351781\|gb\|AB... gi\|166010361, gi... - gi\|166010361\|ref\|ZP_02231259.1\| glutathione synthase [Yersinia pestis biovar Antiqua str. E1979001],... gi\|165925090, gi... - gi\|165925090\|ref\|ZP_02220922.1\| glutathione synthase [Yersinia pestis biovar Orientalis str. F199101... GSHB_YERPE - Glutathione synthetase OS=Yersinia pestis GN=gshB PE=3 SV=1 gi\|167399834, gi... - gi\|167399834\|ref\|ZP_02305352.1\| glutathione synthase [Yersinia pestis biovar Antiqua str. UG05-0454]... Q666P1\|Q666P1_YERPS - Glutathione synthetase - Yersinia pseudotuberculosis gi\|166212824, gi... - gi\|166212824\|ref\|ZP_02238859.1\| glutathione synthase [Yersinia pestis biovar Antiqua str. B42003004]... gi\|167426710, gi... - gi\|167426710\|ref\|ZP_02318463.1\| glutathione synthase [Yersinia pestis biovar Mediaevalis str. K19730... gi\|166960167, gi... - gi\|167422030\|ref\|ZP_02313783.1\| glutathione synthase [Yersinia pestis biovar Orientalis str. MG05-10...	285.0	[0..1]	[316..1]
gi\|78363510, gi\|... - gi\|78485224\|ref\|YP_391149.1\| carbamoyl-phosphate synthase, large subunit [Thiomicrospira crunogena X...	285.0	[0..3]	[316..563]
GSHB_VIBVU - Glutathione synthetase OS=Vibrio vulnificus (strain CMCP6) GN=gshB PE=3 SV=1 GSHB_VIBVU - Glutathione synthetase OS=Vibrio vulnificus GN=gshB PE=3 SV=1	284.0	[0..1]	[316..1]
gi\|75819935, gi\|... - gi\|75819935\|ref\|ZP_00749996.1\| COG0458: Carbamoylphosphate synthase large subunit (split gene in MJ)...	284.0	[0..3]	[316..462]
gi\|90438592, gi\|... - gi\|90580290\|ref\|ZP_01236097.1\| glutathione synthetase [Vibrio angustum S14], gi\|90438592\|gb\|EAS63776...	284.0	[0..1]	[316..1]
gi\|153950930, gi... - gi\|77630183\|ref\|ZP_00792769.1\| COG0189: Glutathione synthase/Ribosomal protein S6 modification enzym...	284.0	[0..1]	[316..1]
gi\|116215779, gi... - gi\|75822523\|ref\|ZP_00752133.1\| COG0458: Carbamoylphosphate synthase large subunit (split gene in MJ)...	284.0	[0..3]	[316..32]
gi\|75816491, gi\|... - gi\|75816491\|ref\|ZP_00746960.1\| COG0458: Carbamoylphosphate synthase large subunit (split gene in MJ)...	284.0	[0..3]	[316..560]

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Predicted Domain #1

Region A:
Residues: [1-316]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIKLGIVMDP IANINIKKDS SFAMLLEAQR RGYELHYMEM GDLYLINGEA RAHTRTLNVK  60
   61 QNYEEWFSFV GEQDLPLADL DVILMRKDPP FDTEFIYATY ILERAEEKGT LIVNKPQSLR 120
  121 DCNEKLFTAW FSDLTPETLV TRNKAQLKAF WEKHSDIILK PLDGMGGASI FRVKEGDPNL 180
  181 GVIAETLTEH GTRYCMAQNY LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG 240
  241 RGEPRPLTES DWKIARQIGP TLKEKGLIFV GLDIIGDRLT EINVTSPTCI REIEAEFPVS 300
  301 ITGMLMDAIE ARLQQQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	75.69897
Match:	1gsaA
Description:	STRUCTURE OF GLUTATHIONE SYNTHETASE COMPLEXED WITH ADP AND GLUTATHIONE
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glutathione synthase activity	3.18460876432532	bayes_pls_golite062009
catalytic activity	2.5009559386228	bayes_pls_golite062009
glutathione binding	2.38364045371863	bayes_pls_golite062009
ligase activity	2.15409885945916	bayes_pls_golite062009
succinate-CoA ligase activity	1.52785506428706	bayes_pls_golite062009
ligase activity, forming carbon-sulfur bonds	0.935432886482705	bayes_pls_golite062009
acid-amino acid ligase activity	0.599203164789408	bayes_pls_golite062009
CoA-ligase activity	0.51228075142462	bayes_pls_golite062009
acid-thiol ligase activity	0.448084116680826	bayes_pls_golite062009
ligase activity, forming carbon-carbon bonds	0.351267830197198	bayes_pls_golite062009
succinate-CoA ligase (ADP-forming) activity	0.324506681366243	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.239594107637156	bayes_pls_golite062009
CoA carboxylase activity	0.185924207432397	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: