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View Structure Prediction Details

Protein: rfc4
Organism: Schizosaccharomyces pombe
Length: 342 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for rfc4.

Description E-value Query
Range
Subject
Range
gi|85102751 - gi|85102751|ref|XP_961381.1| hypothetical protein [Neurospora crassa OR74A]
gi|28922926, gi|... - gi|32412616|ref|XP_326788.1| hypothetical protein [Neurospora crassa], gi|28922926|gb|EAA32145.1| hy...
236.0 [0..17] [342..511]
gi|145016723, gi... - gi|39972449|ref|XP_367615.1| conserved hypothetical protein [Magnaporthe grisea 70-15], gb|EAA53249....
229.0 [0..4] [342..17]
RFC2_CHICK - Replication factor C subunit 2 OS=Gallus gallus GN=RFC2 PE=2 SV=1
227.0 [0..5] [337..25]
RFC2_BOVIN - Replication factor C subunit 2 OS=Bos taurus GN=RFC2 PE=2 SV=1
227.0 [0..6] [337..19]
gi|28950096 - gi|28950096|emb|CAD70859.1| probable REPLICATION FACTOR C (40 KDA SUBUNIT) [Neurospora crassa]
225.0 [0..17] [342..29]
RFC4_ORYSJ - Replication factor C subunit 4 OS=Oryza sativa subsp. japonica GN=RFC4 PE=2 SV=2
225.0 [0..5] [338..1]
gi|73957665 - gi|73957665|ref|XP_546916.2| PREDICTED: similar to replication factor C 2 (40kD) isoform 1 [Canis fa...
224.0 [0..2] [337..15]
gi|47124709, gi|... - gi|47124709|gb|AAH70622.1| MGC81391 protein [Xenopus laevis], gi|147904320|ref|NP_001084837.1| hypot...
223.0 [0..5] [332..15]
gi|109066327 - gi|109066327|ref|XP_001082511.1| PREDICTED: replication factor C 2 (40kD) isoform 2 [Macaca mulatta]
223.0 [0..5] [337..18]
gi|115928698, gi... - gi|115928698|ref|XP_001184083.1| PREDICTED: similar to replication factor C, 40-kDa subunit [Strongy...
223.0 [0..2] [337..14]

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Predicted Domain #1
Region A:
Residues: [1-342]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNAVSSSVF GEKNNSVAYE LPWVEKYRPI VLDDIVGNEE TIDRLKVIAK EGNMPHLVIS  60
   61 GMPGIGKTTS ILCLAHALLG PAYKEGVLEL NASDERGIDV VRNRIKAFAQ KKVILPPGRH 120
  121 KIIILDEADS MTAGAQQALR RTMEIYSNTT RFALACNQSN KIIEPIQSRC AILRYSRLTD 180
  181 QQVLQRLLNI CKAEKVNYTD DGLAALIMTA EGDMRQAVNN LQSTVAGFGL VNGENVFRVA 240
  241 DQPSPVAIHA MLTACQSGNI DVALEKLQGI WDLGFSAVDI VTNMFRVVKT MDSIPEFSRL 300
  301 EMLKEIGQTH MIILEGVQTL LQLSGLVCRL AKSQMKPESF II

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 52.30103
Match: 1sxjB
Description: Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
DNA clamp loader activity 6.51495346697904 bayes_pls_golite062009
protein-DNA loading ATPase activity 6.40748863504898 bayes_pls_golite062009
DNA-dependent ATPase activity 2.72462789867829 bayes_pls_golite062009
binding 2.64537357529343 bayes_pls_golite062009
mismatched DNA binding 2.51625633651862 bayes_pls_golite062009
DNA helicase activity 2.2817164193758 bayes_pls_golite062009
pyrophosphatase activity 2.13284212711072 bayes_pls_golite062009
ATP-dependent DNA helicase activity 1.88455339138556 bayes_pls_golite062009
1.85506769032946 bayes_pls_golite062009
helicase activity 1.70242731830224 bayes_pls_golite062009
guanine/thymine mispair binding 1.69026972999845 bayes_pls_golite062009
DNA insertion or deletion binding 1.68890613599528 bayes_pls_golite062009
RNA helicase activity 1.63960194106079 bayes_pls_golite062009
ATP-dependent RNA helicase activity 1.61735400124552 bayes_pls_golite062009
RNA-dependent ATPase activity 1.60404584546501 bayes_pls_golite062009
nucleic acid binding 1.49633029954726 bayes_pls_golite062009
structure-specific DNA binding 1.2539059700735 bayes_pls_golite062009
purine ribonucleotide binding 1.21658213854831 bayes_pls_golite062009
ribonucleotide binding 1.21652144153182 bayes_pls_golite062009
purine nucleotide binding 1.21571071905795 bayes_pls_golite062009
nucleotide binding 1.20347573865566 bayes_pls_golite062009
ATP-dependent helicase activity 1.19311444180077 bayes_pls_golite062009
purine NTP-dependent helicase activity 1.19311444180077 bayes_pls_golite062009
motor activity 1.14667913749579 bayes_pls_golite062009
microtubule motor activity 1.1277447806079 bayes_pls_golite062009
double-stranded DNA binding 1.117585097627 bayes_pls_golite062009
catalytic activity 1.00104173493322 bayes_pls_golite062009
transcription regulator activity 0.989226939888218 bayes_pls_golite062009
DNA-directed DNA polymerase activity 0.971476020912368 bayes_pls_golite062009
DNA binding 0.967303417236485 bayes_pls_golite062009
DNA polymerase activity 0.928091843881235 bayes_pls_golite062009
guanyl nucleotide binding 0.656009904481476 bayes_pls_golite062009
guanyl ribonucleotide binding 0.64416740142962 bayes_pls_golite062009
GTP binding 0.643084645864898 bayes_pls_golite062009
single base insertion or deletion binding 0.629563765934841 bayes_pls_golite062009
single-stranded DNA binding 0.568457189190858 bayes_pls_golite062009
cytoskeletal protein binding 0.500462538667228 bayes_pls_golite062009
GTPase activity 0.406399103964327 bayes_pls_golite062009
DNA strand annealing activity 0.283577486331079 bayes_pls_golite062009
protein binding 0.170837278876362 bayes_pls_golite062009
nucleoside-triphosphatase activity 0.147579541298736 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides 0.113504049290503 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 0.111293884322157 bayes_pls_golite062009
structural constituent of ribosome 0.089234257580272 bayes_pls_golite062009
3'-5' DNA helicase activity 0.0769112340058804 bayes_pls_golite062009
transcription factor activity 0.0434163635422169 bayes_pls_golite062009
lysine N-acetyltransferase activity 0.0320943071400439 bayes_pls_golite062009
histone acetyltransferase activity 0.0320943071400439 bayes_pls_golite062009
double-strand/single-strand DNA junction binding 0.0136229525922795 bayes_pls_golite062009
actin binding 0.00482938540552702 bayes_pls_golite062009

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