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View Structure Prediction Details

Protein: PIF1
Organism: Saccharomyces cerevisiae
Length: 859 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PIF1.

Description E-value Query
Range
Subject
Range
gi|130196, gi|41... - pir||A29457 PIF1 protein precursor - yeast (Saccharomyces cerevisiae), gi|4178|emb|CAA28953.1| unnam...
0.0 [1..859] [1..857]
gi|15229268, gi|... - gi|7263610|emb|CAB81576.1| putative protein [Arabidopsis thaliana], gi|15229268|ref|NP_189921.1| hel...
0.0 [127..808] [70..830]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
7.0E-93 [83..783] [171..800]
PIF1_SCHPO - ATP-dependent DNA helicase pfh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pfh1 PE=1 ...
pfh1 - pif1 helicase homolog Pfh1
2.0E-92 [83..783] [171..800]

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Predicted Domain #1
Region A:
Residues: [1-133]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF IQAQLKHPSI  60
   61 LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED PVDKKPMRDK NVMNFINKDS 120
  121 PLSWNDMFKP SII

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2
Region A:
Residues: [134-236]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QPPQLISENS FDQSSQKKSR STGFKNPLRP ALKKESSFDE LQNNSISQER SLEMINENEK  60
   61 KKMQFGEKIA VLTQRPSFTE LQNDQDDSNL NPHNGVKVKI PIC

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 3.221849
Match: 1fts__
Description: Signal recognition particle receptor, FtsY; GTPase domain of the signal recognition particle receptor FtsY
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 2.5815673740955 bayes_pls_golite062009
nucleic acid binding 2.18361843860268 bayes_pls_golite062009
DNA binding 1.93283541470819 bayes_pls_golite062009
transcription regulator activity 1.86711849769952 bayes_pls_golite062009
pyrophosphatase activity 1.22434861137232 bayes_pls_golite062009
DNA-dependent ATPase activity 1.00145424382707 bayes_pls_golite062009
catalytic activity 0.942640875796745 bayes_pls_golite062009
helicase activity 0.930824665371921 bayes_pls_golite062009
transcription factor activity 0.737538356360809 bayes_pls_golite062009
transcription repressor activity 0.723025725174193 bayes_pls_golite062009
DNA helicase activity 0.525155854231881 bayes_pls_golite062009
ATP-dependent helicase activity 0.364091846246035 bayes_pls_golite062009
purine NTP-dependent helicase activity 0.364091846246035 bayes_pls_golite062009
protein binding 0.201869978778282 bayes_pls_golite062009
structure-specific DNA binding 0.164280007805333 bayes_pls_golite062009
ATP-dependent DNA helicase activity 0.0142902906813618 bayes_pls_golite062009

Predicted Domain #3
Region A:
Residues: [237-515]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LSKEQESIIK LAENGHNIFY TGSAGTGKSI LLREMIKVLK GIYGRENVAV TASTGLAACN  60
   61 IGGITIHSFA GIGLGKGDAD KLYKKVRRSR KHLRRWENIG ALVVDEISML DAELLDKLDF 120
  121 IARKIRKNHQ PFGGIQLIFC GDFFQLPPVS KDPNRPTKFA FESKAWKEGV KMTIMLQKVF 180
  181 RQRGDVKFID MLNRMRLGNI DDETEREFKK LSRPLPDDEI IPAELYSTRM EVERANNSRL 240
  241 SKLPGQVHIF NAIDGGALED EELKERLLQN FLAPKELHL

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 37.69897
Match: 1uaaA_
Description: DEXX box DNA helicase
Matching Structure (courtesy of the PDB):

Predicted Domain #4
Region A:
Residues: [516-634]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KVGAQVMMVK NLDATLVNGS LGKVIEFMDP ETYFCYEALT NDPSMPPEKL ETWAENPSKL  60
   61 KAAMEREQSD GEESAVASRK SSVKEGFAKS DIGEPVSPLD SSVFDFMKRV KTDDEVVLE

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 37.69897
Match: 1uaaA_
Description: DEXX box DNA helicase
Matching Structure (courtesy of the PDB):

Predicted Domain #5
Region A:
Residues: [635-778]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NIKRKEQLMQ TIHQNSAGKR RLPLVRFKAS DMSTRMVLVE PEDWAIEDEN EKPLVSRVQL  60
   61 PLMLAWSLSI HKSQGQTLPK VKVDLRRVFE KGQAYVALSR AVSREGLQVL NFDRTRIKAH 120
  121 QKVIDFYLTL SSAESAYKQL EADE

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #6
Region A:
Residues: [779-859]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QVKKRKLDYA PGPKYKAKSK SKSNSPAPIS ATTQSNNGIA AMLQRHSRKR FQLKKESNSN  60
   61 QVHSLVSDEP RGQDTEDHIL E

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle