YRC Public Data Repository - Protein Structure Prediction

Protein:	MST1
Organism:	Saccharomyces cerevisiae
Length:	462 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MST1.

Description	E-value	Query Range	Subject Range
SYT_ECOK1 - Threonine--tRNA ligase OS=Escherichia coli O1:K1 / APEC GN=thrS PE=3 SV=1 SYT_ECOLI - Threonyl-tRNA synthetase (EC 6.1.1.3) (Threonine--tRNA ligase) (ThrRS) - Escherichia coli SYT_SHISS - Threonine--tRNA ligase OS=Shigella sonnei (strain Ss046) GN=thrS PE=3 SV=1 SYT_SHIFL - Threonine--tRNA ligase OS=Shigella flexneri GN=thrS PE=3 SV=1 gi\|24112885, gi\|... - gi\|24112885\|ref\|NP_707395.1\| threonyl-tRNA synthetase [Shigella flexneri 2a str. 301], gi\|24051829\|g... EG11001 - threonyl-tRNA synthetase / Threonine--tRNA ligase, autogenously regulated; binds Zn(II) SYT_ECOL6 - Threonine--tRNA ligase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=thrS PE=3 S... gi\|30063014, gi\|... - gi\|30063014\|ref\|NP_837185.1\| threonyl-tRNA synthetase [Shigella flexneri 2a str. 2457T], gi\|30041263... SYT_ECOL6 - Threonine--tRNA ligase OS=Escherichia coli O6 GN=thrS PE=3 SV=1 sp\|B7USA2\|SYT_ECO27 - Threonyl-tRNA synthetase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=thrS PE=3 SV=1 SYT_ECOSM - Threonine--tRNA ligase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=thrS PE=3 SV=1 SYT_ECODH - Threonine--tRNA ligase OS=Escherichia coli (strain K12 / DH10B) GN=thrS PE=3 SV=1 gi\|190908082, gi... - gi\|191171752\|ref\|ZP_03033299.1\| threonyl-tRNA synthetase [Escherichia coli F11], gi\|190908082\|gb\|EDV... gi\|188492265, gi... - gi\|188492265\|ref\|ZP_02999535.1\| threonyl-tRNA synthetase [Escherichia coli 53638], gi\|188487464\|gb\|E... gi\|89108559, gi\|... - gi\|89108559\|ref\|AP_002339.1\| threonyl-tRNA synthetase [Escherichia coli str. K-12 substr. W3110], gi... SYT_ECO7I - Threonine--tRNA ligase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=thrS PE=3 SV=1 gi\|242377442, gi... - gi\|251785169\|ref\|YP_002999473.1\| hypothetical protein B21_01677 [Escherichia coli BL21], gi\|24237744... SYT_ECOUT - Threonine--tRNA ligase OS=Escherichia coli (strain UTI89 / UPEC) GN=thrS PE=3 SV=1 SYT_ECO81 - Threonine--tRNA ligase OS=Escherichia coli O81 (strain ED1a) GN=thrS PE=3 SV=1 gi\|254288567, gi... - gi\|254288567\|ref\|YP_003054315.1\| threonyl-tRNA synthetase [Escherichia coli BL21(DE3)], gi\|253977874... SYT_ECOL5 - Threonine--tRNA ligase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=thrS PE=3 SV=1 gi\|237705668, gi... - gi\|237705668\|ref\|ZP_04536149.1\| threonyl-tRNA synthetase [Escherichia sp. 3_2_53FAA], gi\|226900425\|g... EG11001 - threonyl-tRNA synthetase / Threonine--tRNA ligase, autogenously regulated; binds Zn(II) SYT_SHIF8 - Threonine--tRNA ligase OS=Shigella flexneri serotype 5b (strain 8401) GN=thrS PE=3 SV=1 SYT_ECOLU - Threonine--tRNA ligase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=thrS PE=3 SV=1 SYT_ECOBW - Threonine--tRNA ligase OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=thrS PE=3 SV=1 gi\|194422590, gi... - gi\|194438580\|ref\|ZP_03070669.1\| threonyl-tRNA synthetase [Escherichia coli 101-1], gi\|194422590\|gb\|E... gi\|194416606, gi... - gi\|194435142\|ref\|ZP_03067376.1\| threonyl-tRNA synthetase [Shigella dysenteriae 1012], gi\|194416606\|g... SYT_ECO45 - Threonine--tRNA ligase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=thrS PE=3 SV=1 gi\|254161779, gi... - gi\|254161779\|ref\|YP_003044887.1\| threonyl-tRNA synthetase [Escherichia coli B str. REL606], gi\|25397... SYT_ECO27 - Threonine--tRNA ligase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=thrS PE=3 SV=1	0.0	[1..461]	[199..635]
gi\|43066 - gi\|43066\|emb\|CAA23560.1\| unnamed protein product [Escherichia coli]	0.0	[1..461]	[199..635]
gi\|197241161, gi... - gi\|197262906\|ref\|ZP_03162980.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... SYT_SALTY - Threonine--tRNA ligase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=thrS PE=3 ... SYT_SALHS - Threonine--tRNA ligase OS=Salmonella heidelberg (strain SL476) GN=thrS PE=3 SV=1 SYT_SALTY - Threonyl-tRNA synthetase OS=Salmonella typhimurium GN=thrS PE=3 SV=1 gi\|168244449, gi... - gi\|205336802\|gb\|EDZ23566.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar He... gi\|205329759, gi... - gi\|205329759\|gb\|EDZ16523.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar 4,... lcl\|NC_016856.1_... - [gene=thrS] [protein=threonyl-tRNA synthetase] [protein_id=YP_005237194.1] [location=1422178..142410... SYT_SALTY - Threonine--tRNA ligase OS=Salmonella typhimurium GN=thrS PE=3 SV=1	0.0	[1..461]	[199..635]
SYT_SALTI - Threonyl-tRNA synthetase OS=Salmonella typhi GN=thrS PE=3 SV=1 gi\|199602680, gi... - gi\|200390235\|ref\|ZP_03216846.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... SYT_SALA4 - Threonine--tRNA ligase OS=Salmonella agona (strain SL483) GN=thrS PE=3 SV=1 SYT_SALSV - Threonine--tRNA ligase OS=Salmonella schwarzengrund (strain CVM19633) GN=thrS PE=3 SV=1 gi\|205341550, gi... - gi\|205341550\|gb\|EDZ28314.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar We... gi\|213852224 - gi\|213852224\|ref\|ZP_03381756.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213426331 - gi\|213426331\|ref\|ZP_03359081.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213423451 - gi\|213423451\|ref\|ZP_03356434.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|204927743, gi... - gi\|204927743\|ref\|ZP_03218944.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|168239001, gi... - gi\|197288252\|gb\|EDY27637.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Sc... SYT_SALTI - Threonine--tRNA ligase OS=Salmonella typhi GN=thrS PE=3 SV=1 gi\|167552305, gi... - gi\|205323017\|gb\|EDZ10856.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Sa... gi\|29141677, gi\|... - gi\|29141677\|ref\|NP_805019.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... gi\|16760562 - gi\|16760562\|ref\|NP_456179.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... gi\|238910851 - gi\|238910851\|ref\|ZP_04654688.1\| threonyl-tRNA synthetase [Salmonella enterica subsp. enterica serova...	0.0	[1..461]	[199..635]
gi\|187776029, gi... - gi\|187776029\|ref\|ZP_02799987.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4196], gi\|... gi\|188016225, gi... - gi\|188024843\|ref\|ZP_02774486.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4113], gi\|... gi\|189010441, gi... - gi\|189010441\|ref\|ZP_02807141.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4076], gi\|... gi\|189356196, gi... - gi\|189402338\|ref\|ZP_02781727.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4401], gi\|... gi\|189367509, gi... - gi\|189367509\|gb\|EDU85925.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4501], gi\|1687... gi\|189372060, gi... - gi\|189372060\|gb\|EDU90476.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC869], gi\|16878... gi\|189377572, gi... - gi\|189377572\|gb\|EDU95988.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC508], gi\|16880... gi\|15831680, gi\|... - gi\|15831680\|ref\|NP_310453.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. Sakai], gi\|1336... gi\|254793336, gi... - gi\|254793336\|ref\|YP_003078173.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. TW14359], g... SYT_ECO57 - Threonine--tRNA ligase OS=Escherichia coli O157:H7 GN=thrS PE=3 SV=1 gi\|209768834, gi... - pir\|\|F85780 threonine tRNA synthetase [imported] - Escherichia coli (strain O157:H7, substrain EDL9... gi\|15802131, gi\|... - gi\|15802131\|ref\|NP_288153.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 EDL933], gi\|12515733... gi\|217318337, gi... - gi\|217328990\|ref\|ZP_03445071.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. TW14588], gi... gi\|189360618, gi... - gi\|189403439\|ref\|ZP_02795487.2\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4486], gi\|... gi\|208725260, gi... - gi\|208810744\|ref\|ZP_03252620.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4206], gi\|... gi\|208730990, gi... - gi\|208816647\|ref\|ZP_03257767.1\| threonyl-tRNA synthetase [Escherichia coli O157:H7 str. EC4045], gi\|... SYT_ECO5E - Threonine--tRNA ligase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=thrS PE=3 SV=1	0.0	[1..461]	[199..635]
SYT_VIBCM - Threonine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain M66-2) GN=thrS PE=3 SV=1 SYT_VIBCH - Threonine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN=t... gi\|229353090, gi... - gi\|229510645\|ref\|ZP_04400125.1\| threonyl-tRNA synthetase [Vibrio cholerae B33], gi\|229353090\|gb\|EEO1... gi\|229356910, gi... - gi\|229506559\|ref\|ZP_04396068.1\| threonyl-tRNA synthetase [Vibrio cholerae BX 330286], gi\|229356910\|g... gi\|229606038, gi... - gi\|229606038\|ref\|YP_002876742.1\| threonyl-tRNA synthetase [Vibrio cholerae MJ-1236], gi\|229372524\|gb... gi\|229345260, gi... - gi\|229517224\|ref\|ZP_04406669.1\| threonyl-tRNA synthetase [Vibrio cholerae RC9], gi\|229345260\|gb\|EEO1... gi\|126511981, gi... - gi\|153817556\|ref\|ZP_01970223.1\| threonyl-tRNA synthetase [Vibrio cholerae NCTC 8457], gi\|126511981\|g... gi\|153221646, gi... - gi\|153221646\|ref\|ZP_01952571.1\| threonyl-tRNA synthetase [Vibrio cholerae MAK 757], gi\|124119556\|gb\|... gi\|254850514, gi... - gi\|254850514\|ref\|ZP_05239864.1\| threonyl-tRNA synthetase [Vibrio cholerae MO10], gi\|254846219\|gb\|EET... SYT_VIBCH - Threonine--tRNA ligase OS=Vibrio cholerae GN=thrS PE=3 SV=1	0.0	[1..462]	[200..637]
SYT_PASMU - Threonyl-tRNA synthetase OS=Pasteurella multocida GN=thrS PE=3 SV=1 SYT_PASMU - Threonine--tRNA ligase OS=Pasteurella multocida (strain Pm70) GN=thrS PE=3 SV=1	0.0	[1..461]	[199..635]
SYT_YERPP - Threonine--tRNA ligase OS=Yersinia pestis (strain Pestoides F) GN=thrS PE=3 SV=1 gi\|167424849, gi... - gi\|167424849\|ref\|ZP_02316602.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Mediaevalis str. K1... SYT_YERPY - Threonine--tRNA ligase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=thrS PE=3 SV=1 SYT_YERPB - Threonine--tRNA ligase OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=thrS PE=3 SV=1 SYT_YERPE - Threonine--tRNA ligase OS=Yersinia pestis GN=thrS PE=3 SV=1 gi\|165927513, gi... - gi\|165927513\|ref\|ZP_02223345.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Orientalis str. F19... gi\|165989259, gi... - gi\|166011794\|ref\|ZP_02232692.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Antiqua str. E19790... gi\|166207632, gi... - gi\|166210452\|ref\|ZP_02236487.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Antiqua str. B42003... gi\|149292071, gi... - gi\|149365658\|ref\|ZP_01887693.1\| threonyl-tRNA synthetase [Yersinia pestis CA88-4125], gi\|149292071\|g... gi\|167049601, gi... - gi\|167400748\|ref\|ZP_02306254.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Antiqua str. UG05-0... gi\|45442011, gi\|... - gi\|45442011\|ref\|NP_993550.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Microtus str. 91001], ... gi\|22125796, gi\|... - gi\|22125796\|ref\|NP_669219.1\| threonyl-tRNA synthetase [Yersinia pestis KIM], gi\|21958722\|gb\|AAM85470... gi\|115348133, gi... - gi\|218929522\|ref\|YP_002347397.1\| threonyl-tRNA synthetase [Yersinia pestis CO92], gi\|16122654\|ref\|NP... SYT_YERPS - Threonine--tRNA ligase OS=Yersinia pseudotuberculosis serotype I (strain IP32953) GN=thrS PE=3 SV=1 gi\|229694326, gi... - gi\|229837960\|ref\|ZP_04458119.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Orientalis str. PEX... gi\|229701883, gi... - gi\|229895121\|ref\|ZP_04510297.1\| threonyl-tRNA synthetase [Yersinia pestis Pestoides A], gi\|229701883... gi\|229688069, gi... - gi\|229898521\|ref\|ZP_04513666.1\| threonyl-tRNA synthetase [Yersinia pestis biovar Orientalis str. Ind... SYT_YERPN - Threonine--tRNA ligase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=thrS PE=3 SV=1 SYT_YERPS - Threonine--tRNA ligase OS=Yersinia pseudotuberculosis GN=thrS PE=3 SV=1 SYT_YERPG - Threonine--tRNA ligase OS=Yersinia pestis bv. Antiqua (strain Angola) GN=thrS PE=3 SV=1 SYT_YERP3 - Threonine--tRNA ligase OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=thrS PE=3 S... gi\|89105335, gi\|... - gi\|89105335\|ref\|ZP_01177856.1\| COG0441: Threonyl-tRNA synthetase [Yersinia pestis biovar Orientalis ... SYT_YERPA - Threonine--tRNA ligase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=thrS PE=3 SV=1	0.0	[2..461]	[200..635]
gi\|11271143 - pir\|\|E81167 threonyl-tRNA synthetase NMB0720 [imported] - Neisseria meningitidis (strain MC58 serogr... SYT_NEIMB - Threonine--tRNA ligase OS=Neisseria meningitidis serogroup B (strain MC58) GN=thrS PE=3 SV=1 SYT_NEIMB - Threonine--tRNA ligase OS=Neisseria meningitidis serogroup B GN=thrS PE=3 SV=1	0.0	[2..458]	[199..630]

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Predicted Domain #1

Region A:
Residues: [1-340]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM TDPLSPGSMF  60
   61 FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW EKSGHWENYA DDMFKVETTD 120
  121 EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL PLRFSDFSPL HRNEASGALS GLTRLRKFHQ 180
  181 DDGHIFCTPS QVKSEIFNSL KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK 240
  241 VWNHAEQVLK EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER 300
  301 FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	895.9897
Match:	1qf6A_
Description:	Threonyl-tRNA synthetase (ThrRS), C-terminal domain; Threonyl-tRNA synthetase (ThrRS), N-terminal 'additional' domain; Threonyl-tRNA synthetase (ThrRS), second 'additional' domain; Threonyl-tRNA synthetase (ThrRS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.89468709683036	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.89468709683036	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.88410036819636	bayes_pls_golite062009
ligase activity	6.92073480193262	bayes_pls_golite062009
threonine-tRNA ligase activity	6.47381405106149	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	2.99105702052936	bayes_pls_golite062009
serine-tRNA ligase activity	2.46505535069103	bayes_pls_golite062009
glycine-tRNA ligase activity	2.29229136935761	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
alanine-tRNA ligase activity	1.82594054955281	bayes_pls_golite062009
lysine-tRNA ligase activity	1.72324031407565	bayes_pls_golite062009
histidine-tRNA ligase activity	1.72215103383322	bayes_pls_golite062009
binding	0.966824335333342	bayes_pls_golite062009
asparagine-tRNA ligase activity	0.921492123574877	bayes_pls_golite062009
transcription regulator activity	0.375542987753806	bayes_pls_golite062009
nucleic acid binding	0.281662799812287	bayes_pls_golite062009
protein binding	0.0804954454681041	bayes_pls_golite062009
DNA binding	0.0309732052586011	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.00572514744734753	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [341-462]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 WPFWLNPYQA VIIPVNTKNV QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN  60
   61 EPVGYRIKSA ILKNYSYLII VGDEEVQLQK YNIRERDNRK SFEKLTMSQI WEKFIELEKN 120
  121 YK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	895.9897
Match:	1qf6A_
Description:	Threonyl-tRNA synthetase (ThrRS), C-terminal domain; Threonyl-tRNA synthetase (ThrRS), N-terminal 'additional' domain; Threonyl-tRNA synthetase (ThrRS), second 'additional' domain; Threonyl-tRNA synthetase (ThrRS)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: