YRC Public Data Repository - Protein Structure Prediction

Protein:	TOS3
Organism:	Saccharomyces cerevisiae
Length:	560 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TOS3.

Description	E-value	Query Range	Subject Range
gi\|322596, gi\|11... - gi\|6714479\|gb\|AAF26165.1\|AC008261_22 putative SNF1-related protein kinase [Arabidopsis thaliana], pi...	0.0	[49..544]	[18..467]
TOS3_YEAST - Serine/threonine-protein kinase TOS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOS... TOS3 - Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation a...	0.0	[1..560]	[1..560]
gi\|125543425 - gi\|125543425\|gb\|EAY89564.1\| hypothetical protein OsI_11096 [Oryza sativa Indica Group] gi\|28201244, gi\|... - gi\|28201244\|dbj\|BAC56590.1\| SnRK1b protein kinase [Oryza sativa Japonica Group], gi\|169244479\|gb\|ACA... gi\|4107007, gi\|4... - gi\|4107007\|dbj\|BAA36297.1\| OSK3 [Oryza sativa], gi\|4107003\|dbj\|BAA36295.1\| OSK5 [Oryza sativa]	0.0	[47..523]	[14..433]
gi\|7672782 - gi\|7672782\|gb\|AAF66639.1\|AF143743_1 SNF1 [Lycopersicon esculentum]	0.0	[46..538]	[15..463]
gi\|4567091 - gi\|4567091\|gb\|AAD23582.1\|AF128443_1 SNF-1-like serine/threonine protein kinase [Glycine max]	0.0	[46..498]	[16..416]
gi\|575292 - gi\|575292\|emb\|CAA57898.1\| SNF1-related protein kinase [Hordeum vulgare subsp. vulgare]	0.0	[53..538]	[1..432]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[49..520]	[7..421]

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Predicted Domain #1

Region A:
Residues: [1-41]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVLLKEPVQP LPRSSLLYNN ASNSSSRIKE TRKVKLLYNP L

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [97-119]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ENPRVNQEIE VMKRCHHENV VEL

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [137-390]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YCSRGPVKWC PENKMEIKAV GPSILTFQQS RKVVLDVVSG LEYLHSQGIT HRDIKPSNLL  60
   61 ISSNGTVKIS DFGVAMSTAT GSTNIQSSHE QLLKSRALGT PAFFAPELCS TEKEYSCSSA 120
  121 IDIWSLGVTI YCLLFGKLPF NANSGLELFD SIINKPLEFP SYEEMLNGAT SGITMEEYTD 180
  181 AKDLLKKLLQ KDPDKRIKLA DIKVHPFMCH YGKSDAASVL TNLETFHELK VSPPSSCKRV 240
  241 ELVSLPVNSS FASL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	380.0
Match:	1ctpE_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
protein kinase activity	5.17195961442593	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	5.04467222534257	bayes_pls_golite062009
kinase activity	4.85903980916957	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.46123870227889	bayes_pls_golite062009
transferase activity	3.26787750561728	bayes_pls_golite062009
binding	2.31930216202447	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
protein tyrosine kinase activity	1.3898059094397	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.38496328649031	bayes_pls_golite062009
receptor signaling protein activity	1.27291233344322	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.282849280638519	bayes_pls_golite062009
signal transducer activity	0.270054246024761	bayes_pls_golite062009
molecular transducer activity	0.270054246024761	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.0556163711947139	bayes_pls_golite062009
transmembrane receptor activity	0.00652065412910241	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [42-96]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TKRQILNNFE ILATLGNGQY GKVKLARDLG TGALVAIKIL NRFEKRSGYS LQLKV

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [120-136]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YEILNDPEST KVYLVLE

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [391-439]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DSVYMENFDH NNLRTGADRN STYSPSIYDA NTLSPSAYHN IGSRESSYS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	380.0
Match:	1ctpE_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [440-560]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SFSSFTSSTA FASQISIQDA PAIGDQQCLI GESGSSLRVN SCEFPQYTTM SPVGEYPFES  60
   61 TEASLSSTLT PVGNVPQRIK AHLVEGKSNS KDDLRIEADA SLVFEASDAQ RTRRRMSLYK 120
  121 L

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: