YRC Public Data Repository - Protein Structure Prediction

Protein:	ECM32
Organism:	Saccharomyces cerevisiae
Length:	1121 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ECM32.

Description	E-value	Query Range	Subject Range
ECM32_YEAST - Putative ATP-dependent RNA helicase ECM32 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) G... ECM32 - DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is...	0.0	[1..1121]	[1..1121]
RENT1_NEUCR, RNT... - (Q9HEH1) Regulator of nonsense transcripts 1 homolog, Regulator of nonsense transcripts 1 homolog OS... RENT1_NEUCR - Regulator of nonsense transcripts 1 homolog OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / C...	0.0	[401..1114]	[196..883]
gi\|19577367 - gi\|19577367\|emb\|CAD28448.1\| possible regulator of nonsense transcripts [Aspergillus fumigatus]	0.0	[403..1114]	[149..834]
gi\|3328175, gi\|1... - gi\|3328175\|gb\|AAC26788.1\| nonsense-mediated mRNA decay trans-acting factor [Homo sapiens], gi\|273935...	0.0	[402..1114]	[217..898]
gi\|12836885, gi\|... - gi\|13507601\|ref\|NP_109605.1\| regulator of nonsense transcripts 1 [Mus musculus], gi\|12836885\|gb\|AAK0...	0.0	[402..1114]	[212..893]
gi\|19569060 - gi\|19569060\|gb\|AAL92018.1\| UPF1 [Arabidopsis thaliana]	0.0	[421..1114]	[246..907]

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Predicted Domain #1

Region A:
Residues: [1-382]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDFQCRTCSQ AYDAEQMMKH LSSTRHKTVF DTSNDEDICC EECQDKNIHQ LQIIRFGGED  60
   61 MVLLCNSCFR KEYSETERPS TSYSLQNGSI LKFWEKYVKV RECCCDECGE ESNLNANRNG 120
  121 EVLCDKCLPK SNRAKDFVSE KSGRFLYIYL GLNETQNSTR KPRKKGGRRV GRGKKGRKGA 180
  181 KIKKEKKETF EAKISRIAYE VKKENSTIQS SSSSNLRNFK GFKAVESDPV VAAKVSKSET 240
  241 SRSNPGPSNR NKGKGNKANH KKNSGNGIGK EKERKTNIRN NVRNSQPIPE DRKNTNSHVT 300
  301 TNSGGKGKNE SVDKHQLPQP KALNGNGSGS TNTTGLKKGK KDHAGQKTKG NDKTGNKNPR 360
  361 EAKLNSAGRK NALGKKSNNQ PN

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	8.07
Match:	1i3qA
Description:	RBP1
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [383-461]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KGTSRWTIGS DTESSREPSI SPNENTTSIT KSRNRNKKAS KPTLNEKSKT TTMPKKLETK  60
   61 NQEKNNGKTK DGKLIYEEG

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	10.24
Match:	1l8mA
Description:	No description for 1l8mA was found.

Predicted Domain #3

Region A:
Residues: [462-632]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EPLTRYNTFK STLSYPDLNT YLNDYSFALF LEQKLENEFV QNFNILWPRN EKDTAFIINV  60
   61 EKNNNSELEK LLPANLLALG RPAFNERQPF FFCTQDEQKV WYIFIKELSI QRGKYVLLVE 120
  121 LFSWNNLSLP TKNGSSQFKL LPTSAQTSRI LFAMTRITNP KFIDLLLGQK P

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4

Region A:
Residues: [633-1121]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IKEIYFDNRL KFSSDKLNRS QKTAVEHVLN NSITILQGPP GTGKTSTIEE IIIQVIERFH  60
   61 AFPILCVAAS NIAIDNIAEK IMENRPQIKI LRILSKKKEQ QYSDDHPLGE ICLHNIVYKN 120
  121 LSPDMQVVAN KTRRGEMISK SEDTKFYKEK NRVTNKVVSQ SQIIFTTNIA AGGRELKVIK 180
  181 ECPVVIMDEA TQSSEASTLV PLSLPGIRNF VFVGDEKQLS SFSNIPQLET SLFERVLSNG 240
  241 TYKNPLMLDT QYRMHPKISE FPIKKIYNGE LKDGVTDEQK AWPGVQHPLF FYQCDLGPES 300
  301 RVRSTQRDIV GFTYENKHEC VEIVKIIQIL MLDKKVPLEE IGVITPYSAQ RDLLSDILTK 360
  361 NVVINPKQIS MQQEYDEIEL FNAAGSQGTA GSLQNNVINI INGLHVATVD SFQGHEKSFI 420
  421 IFSCVRNNTE NKIGFLRDKR RLNVALTRAK HGLIVVGNKN VLRKGDPLWK DYITYLEEQE 480
  481 VIFTDLTAY

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	80.30103
Match:	1pjr__
Description:	DEXX box DNA helicase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
structural constituent of ribosome	3.070470376968	bayes_pls_golite062009
structural molecule activity	2.08211451183607	bayes_pls_golite062009
binding	2.00548409930693	bayes_pls_golite062009
translation release factor activity	1.47279606301067	bayes_pls_golite062009
translation termination factor activity	1.47162484856171	bayes_pls_golite062009
nucleic acid binding	1.24151268097078	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA binding	0.829652732561224	bayes_pls_golite062009
transcription regulator activity	0.648798858522413	bayes_pls_golite062009
purine nucleotide binding	0.569910969126728	bayes_pls_golite062009
purine ribonucleotide binding	0.568840867400671	bayes_pls_golite062009
ribonucleotide binding	0.568779924702229	bayes_pls_golite062009
nucleotide binding	0.564275755355315	bayes_pls_golite062009
translation regulator activity	0.493216082928152	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.480200274620277	bayes_pls_golite062009
translation elongation factor activity	0.322643574778	bayes_pls_golite062009
RNA helicase activity	0.2302541318658	bayes_pls_golite062009
ATP binding	0.1860481660648	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
adenyl ribonucleotide binding	0.152859582615242	bayes_pls_golite062009
adenyl nucleotide binding	0.138615225841109	bayes_pls_golite062009
RNA binding	0.0693163519626152	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0530667986761242	bayes_pls_golite062009
pyrophosphatase activity	0.0482442222493492	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.028470639373934	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0265151533232588	bayes_pls_golite062009
RNA-dependent ATPase activity	0.0067235917574795	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: