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View Structure Prediction Details

Protein: DOA4
Organism: Saccharomyces cerevisiae
Length: 926 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for DOA4.

Description E-value Query
Range
Subject
Range
DOA4 - Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated interme...
UBP4_YEAST - Ubiquitin carboxyl-terminal hydrolase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=...
0.0 [1..926] [1..926]
UBP4_KLULA - Ubiquitin carboxyl-terminal hydrolase 4 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 7...
UBP4_KLULA - Ubiquitin carboxyl-terminal hydrolase 4 OS=Kluyveromyces lactis GN=DOA4 PE=3 SV=1
0.0 [14..494] [2..442]
UBP21_MOUSE - Ubiquitin carboxyl-terminal hydrolase 21 OS=Mus musculus GN=Usp21 PE=1 SV=1
5.0E-99 [344..921] [7..557]
gi|6492126, gi|5... - gi|6492126|gb|AAF14190.1|AF106659_1 deubiquitinating enzyme Ubp69 [Rattus norvegicus], gi|51592088|r...
4.0E-96 [342..921] [82..610]

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Predicted Domain #1
Region A:
Residues: [1-184]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS LLDKCIDILS  60
   61 IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI HIIVTTNIPH LSEFAKIKLH 120
  121 KSTSDEGNGN NNNNEFQLMN IYNTLLETLL KDENIAKIKS FIKSSIKQTK LNHEQEECNL 180
  181 MRTG

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #2
Region A:
Residues: [185-335]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SYITSNQLNS LISSSANSAS SQMEILLIDI RSRLEFNKSH IDTKNIICLE PISFKMSYSD  60
   61 HDLEKKSLIT SPNSEIKMFQ SRNLFKFIIL YTDANEYNVK QQSVLLDILV NHSFEKPISD 120
  121 DFTKIFILES GFPGWLKSNY GRQVSSSFPS N

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 14.0
Match: 1d5vA_
Description: Adipocyte-transcription factor FREAC-11 (s12, fkh-14)
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [336-561]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NNIKDDSVYI NGNTSGLSLQ HLPKMSPSIR HSMDDSMKEM LVAPTPLNHL QQQQQQQSDN  60
   61 DHVLKRSSSF KKLFSNYTSP NPKNSNSNLY SISSLSISSS PSPLPLHSPD PVKGNSLPIN 120
  121 YPETPHLWKN SETDFMTNQR EQLNHNSFAH IAPINTKAIT SPSRTATPKL QRFPQTISMN 180
  181 LNMNSNGHSS ATSTIQPSCL SLSNNDSLDH TDVTPTSSHN YDLDFA

[Run NCBI BLAST on this sequence.]

Detection Method: ORFEUS
Confidence: 7.33
Match: 1ldvA
Description: No description for 1ldvA was found.

Predicted functions:

Term Confidence Notes
transcription regulator activity 5.29569167054278 bayes_pls_golite062009
DNA binding 4.90756541371868 bayes_pls_golite062009
nucleic acid binding 4.69183445184264 bayes_pls_golite062009
transcription factor activity 4.14166958134441 bayes_pls_golite062009
binding 3.52874320222492 bayes_pls_golite062009
sequence-specific DNA binding 2.48053910222825 bayes_pls_golite062009
hydrolase activity 2.30467124306022 bayes_pls_golite062009
transcription activator activity 2.29614125067822 bayes_pls_golite062009
transcription repressor activity 2.19033875364989 bayes_pls_golite062009
RNA polymerase II transcription factor activity 1.48055533524841 bayes_pls_golite062009
small conjugating protein ligase activity 1.27763700765562 bayes_pls_golite062009
ubiquitin-protein ligase activity 1.12147035668008 bayes_pls_golite062009
transcription factor binding 0.83705247708153 bayes_pls_golite062009
acid-amino acid ligase activity 0.754042916649094 bayes_pls_golite062009
protein binding 0.753698599635855 bayes_pls_golite062009
structure-specific DNA binding 0.379762425417023 bayes_pls_golite062009
structural molecule activity 0.272316540117209 bayes_pls_golite062009
signal transducer activity 0.120341737642948 bayes_pls_golite062009
molecular transducer activity 0.120341737642948 bayes_pls_golite062009
structural constituent of ribosome 0.0912115683429473 bayes_pls_golite062009

Predicted Domain #4
Region A:
Residues: [562-753]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VGLENLGNSC YMNCIIQCIL GTHELTQIFL DDSYAKHINI NSKLGSKGIL AKYFARLVHM  60
   61 MYKEQVDGSK KISISPIKFK LACGSVNSLF KTASQQDCQE FCQFLLDGLH EDLNQCGSNP 120
  121 PLKELSQEAE ARREKLSLRI ASSIEWERFL TTDFSVIVDL FQGQYASRLK CKVCSHTSTT 180
  181 YQPFTVLSIP IP

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #5
Region A:
Residues: [754-926]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKNSRNNITI EDCFREFTKC ENLEVDEQWL CPHCEKRQPS TKQLTITRLP RNLIVHLKRF  60
   61 DNLLNKNNDF VIYPFLLDLT PFWANDFDGV FPPGVNDDEL PIRGQIPPFK YELYGVACHF 120
  121 GTLYGGHYTA YVKKGLKKGW LYFDDTKYKP VKNKADAINS NAYVLFYHRV YGV

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle