YRC Public Data Repository - Protein Structure Prediction

Protein:	ASNB_ECOLI
Organism:	Escherichia coli
Length:	554 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ASNB_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|91071280, gi\|... - gi\|91209706\|ref\|YP_539692.1\| asparagine synthetase B [Escherichia coli UTI89], gi\|91071280\|gb\|ABE061... gi\|117622874, gi... - gi\|117622874\|ref\|YP_851787.1\| asparagine synthetase B [Escherichia coli APEC O1], gi\|115511998\|gb\|AB... gi\|237707368, gi... - gi\|237707368\|ref\|ZP_04537849.1\| glutamine-hydrolyzing asparagine synthetase B [Escherichia sp. 3_2_5...	552.0	[0..1]	[554..79]
gi\|26107038, gi\|... - gi\|26246639\|ref\|NP_752679.1\| asparagine synthetase B [Escherichia coli CFT073], gi\|26107038\|gb\|AAN79...	551.0	[0..1]	[554..79]
gi\|6691648 - gi\|6691648\|dbj\|BAA89376.1\| unnamed protein product [Moritella marina]	550.0	[0..1]	[554..1]
gi\|218357299, gi... - gi\|218549758\|ref\|YP_002383549.1\| asparagine synthetase B [Escherichia fergusonii ATCC 35469], gi\|218... gi\|157065831, gi... - gi\|75194840\|ref\|ZP_00704910.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col... gi\|169755908, gi... - gi\|170020980\|ref\|YP_001725934.1\| asparagine synthetase B [Escherichia coli ATCC 8739], gi\|169755908\|... gi\|254287530, gi... - gi\|254287530\|ref\|YP_003053278.1\| asparagine synthetase B [Escherichia coli BL21(DE3)], gi\|253976837\|... gi\|251784164, gi... - gi\|251784164\|ref\|YP_002998468.1\| hypothetical protein B21_00622 [Escherichia coli BL21], gi\|24237643... gi\|253972643, gi... - gi\|254160742\|ref\|YP_003043850.1\| asparagine synthetase B [Escherichia coli B str. REL606], gi\|253972... gi\|124499701, gi... - gi\|124529698\|ref\|ZP_01700605.1\| asparagine synthase (glutamine-hydrolyzing) [Escherichia coli B], gi...	548.0	[0..1]	[554..1]
gi\|157156873, gi... - gi\|75189702\|ref\|ZP_00702969.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col...	548.0	[0..1]	[554..1]
gi\|190905760, gi... - gi\|75237584\|ref\|ZP_00721615.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col... gi\|110640885, gi... - gi\|110640885\|ref\|YP_668613.1\| asparagine synthetase B [Escherichia coli 536], gi\|110342477\|gb\|ABG687...	548.0	[0..1]	[554..1]
gi\|75258600, gi\|... - gi\|75258600\|ref\|ZP_00730007.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col... gi\|75227340 - gi\|75227340\|ref\|ZP_00714123.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col... gi\|194412220, gi... - gi\|75209023\|ref\|ZP_00709283.1\| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Escherichia col... gi\|209911184, gi... - gi\|209917925\|ref\|YP_002292009.1\| asparagine synthetase B [Escherichia coli SE11], gi\|209911184\|dbj\|B... gi\|218350834, gi... - gi\|218694102\|ref\|YP_002401769.1\| asparagine synthetase B [Escherichia coli 55989], gi\|218350834\|emb\|...	547.0	[0..1]	[554..1]
gi\|30040395, gi\|... - gi\|30062150\|ref\|NP_836321.1\| asparagine synthetase B [Shigella flexneri 2a str. 2457T], gi\|30040395\|...	546.0	[0..1]	[554..1]
gi\|110804689, gi... - gi\|110804689\|ref\|YP_688209.1\| asparagine synthetase B [Shigella flexneri 5 str. 8401], gi\|110614237\|...	546.0	[0..1]	[554..1]
gi\|74311201, gi\|... - gi\|74311201\|ref\|YP_309620.1\| asparagine synthetase B [Shigella sonnei Ss046], gi\|73854678\|gb\|AAZ8738...	546.0	[0..1]	[554..1]

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Predicted Domain #1

Region A:
Residues: [1-554]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCSIFGVFDI KTDAVELRKK ALELSRLMRH RGPDWSGIYA SDNAILAHER LSIVDVNAGA  60
   61 QPLYNQQKTH VLAVNGEIYN HQALRAEYGD RYQFQTGSDC EVILALYQEK GPEFLDDLQG 120
  121 MFAFALYDSE KDAYLIGRDH LGIIPLYMGY DEHGQLYVAS EMKALVPVCR TIKEFPAGSY 180
  181 LWSQDGEIRS YYHRDWFDYD AVKDNVTDKN ELRQALEDSV KSHLMSDVPY GVLLSGGLDS 240
  241 SIISAITKKY AARRVEDQER SEAWWPQLHS FAVGLPGSPD LKAAQEVANH LGTVHHEIHF 300
  301 TVQEGLDAIR DVIYHIETYD VTTIRASTPM YLMSRKIKAM GIKMVLSGEG SDEVFGGYLY 360
  361 FHKAPNAKEL HEETVRKLLA LHMYDCARAN KAMSAWGVEA RVPFLDKKFL DVAMRINPQD 420
  421 KMCGNGKMEK HILRECFEAY LPASVAWRQK EQFSDGVGYS WIDTLKEVAA QQVSDQQLET 480
  481 ARFRFPYNTP TSKEAYLYRE IFEELFPLPS AAECVPGGPS VACSSAKAIE WDEAFKKMDD 540
  541 PSGRAVGVHQ SAYK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	154.0
Match:	1ct9A
Description:	Asparagine synthetase B, C-terminal domain; Asparagine synthetase B, N-terminal domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
asparagine synthase (glutamine-hydrolyzing) activity	3.86770527955716	bayes_pls_golite062009
asparaginase activity	2.94567124791739	bayes_pls_golite062009
peptidase activity	1.76298553502805	bayes_pls_golite062009
DNA binding	1.66643360311025	bayes_pls_golite062009
nucleic acid binding	1.65897778831304	bayes_pls_golite062009
endopeptidase activity	1.58381740825719	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	1.56290563467621	bayes_pls_golite062009
acid-ammonia (or amide) ligase activity	1.41109692769794	bayes_pls_golite062009
N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity	1.2528157874993	bayes_pls_golite062009
catalytic activity	1.19663085909195	bayes_pls_golite062009
binding	1.18139971903068	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	1.17174228126736	bayes_pls_golite062009
hydrolase activity	1.05106912722792	bayes_pls_golite062009
protein binding	1.03373728327879	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: