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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 5 6 49.7% 0.119 16135
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 15 17 44.4% 0.136 42271
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 1 1 34.8% 0.0421 7729
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 6 7 28.1% 0.0804 29280
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 16 17 26.9% 0.085 67400
[View Peptides]   HHT1, HHT2 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... 1 1 23.5% 0.0214 15356
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 25 25 18.2% 0.0433 195140
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 12 12 18.2% 0.041 94104
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 2 2 14.9% 0.0361 18751
[View Peptides]   RPS7A Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... 1 1 14.7% 0.0153 21622
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 2 2 13.3% 0.0175 35747
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 4 4 10.1% 0.0202 66087
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 1 9.4% 0.00978 33715
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 1 1 7.7% 0.00702 44535
[View Peptides]   Reverse_YOR292C YOR292C SGDID:S000005818, Chr XV from 866579-865650, reverse complement, Uncharacterized ORF, "Hypot... 1 1 7.1% 0.00937 36195
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 1 1 7.1% 0.0121 26503
[View Peptides]   SRX1 Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups i... 1 1 7.1% 0.0229 13854
[View Peptides]   RPS12 Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 1 1 7.0% 0.0203 15472
[View Peptides]   RRN3 Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of ... 2 2 6.7% 0.00927 72388
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 2 2 6.7% 0.00909 69470
[View Peptides]   PUP1 Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after ba... 1 1 5.4% 0.0111 28268
[View Peptides]   RPL17A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... 1 1 5.4% 0.0158 20549
[View Peptides]   RPL19B, RPL19A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similari... 1 1 5.3% 0.0154 21704
[View Peptides]   DAK2 Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress a... 1 1 5.2% 0.00492 62135
[View Peptides]   Reverse_YDR305C HNT2 SGDID:S000002713, Chr IV from 1073482-1073396,1073306-1072740, reverse complement, Verified ORF... 1 1 4.6% 0.0133 24784
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 1 1 4.4% 0.00755 45384
[View Peptides]   ECM31 Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoiso... 1 1 4.2% 0.00931 34465
[View Peptides]   Reverse_YOR323C PRO2 SGDID:S000005850, Chr XV from 922900-921530, reverse complement, Verified ORF, "Gamma-glutamyl ... 1 1 3.7% 0.00636 49741
[View Peptides]   MFB1 Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; in... 1 1 3.2% 0.00625 53168
[View Peptides]   VPS4 AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endos... 1 1 3.2% 0.00665 48172
[View Peptides]   Reverse_YLR052W IES3 SGDID:S000004042, Chr XII from 247202-247954, Verified ORF, "Subunit of the INO80 chromatin rem... 1 1 3.2% 0.0116 28243
[View Peptides]   Reverse_YIL131C FKH1 SGDID:S000001393, Chr IX from 102235-100781, reverse complement, Verified ORF, "Transcription f... 1 1 3.1% 0.00599 53490
[View Peptides]   Reverse_YHR064C SSZ1 SGDID:S000001106, Chr VIII from 227143-225527, reverse complement, Verified ORF, "Hsp70 protein... 1 1 2.6% 0.00539 58238
[View Peptides]   ALK2 Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated ... 1 1 2.5% 0.0043 76372
[View Peptides]   QNS1 Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid ade... 1 1 2.4% 0.00407 80686
[View Peptides]   NOP1 Nucleolar protein, component of the small subunit processome complex, which is required for processi... 1 1 2.4% 0.00889 34465
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 2 3 2.1% 0.00923 111782
[View Peptides]   SWP82 Member of the SWI/SNF chromatin remodeling complex in which it plays an as yet unidentified role; ha... 1 1 2.1% 0.00466 70276
[View Peptides]   SAN1 Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear prote... 1 1 2.1% 0.00476 65618
[View Peptides]   APM1 Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved ... 1 1 2.1% 0.00612 53874
[View Peptides]   TDA11 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 1 1 2.0% 0.00577 56314
[View Peptides]   YGR012W Putative cysteine synthase, localized to the mitochondrial outer membrane 1 1 2.0% 0.00739 42801
[View Peptides]   Reverse_YML035C AMD1 SGDID:S000004498, Chr XIII from 208860-206428, reverse complement, Verified ORF, "AMP deaminase... 1 1 1.9% 0.00358 93302
[View Peptides]   Reverse_YDR085C AFR1 SGDID:S000002492, Chr IV from 616145-614283, reverse complement, Verified ORF, "Alpha-factor ph... 1 1 1.9% 0.00468 71364
[View Peptides]   Reverse_YGR260W TNA1 SGDID:S000003492, Chr VII from 1012491-1014095, Verified ORF, "High affinity nicotinic acid pla... 1 1 1.9% 0.00543 60135
[View Peptides]   ROG3 Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; has similarity to Rod1p; mutation... 1 1 1.8% 0.00396 79709
[View Peptides]   TOS1 Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF tar... 1 1 1.8% 0.00639 47994
[View Peptides]   FRE1 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to u... 1 1 1.6% 0.00424 78854
[View Peptides]   IQG1 Essential protein required for determination of budding pattern, promotes localization of axial mark... 1 1 1.5% 0.00194 172830
[View Peptides]   MSB2 Mucin family member involved in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pa... 1 1 1.5% 0.00222 133114
[View Peptides]   Reverse_YOL089C HAL9 SGDID:S000005449, Chr XV from 153489-150397, reverse complement, Verified ORF, "Putative transc... 1 1 1.5% 0.00282 117926
[View Peptides]   UTP25 Nucleolar protein that cosediments with the 40S ribosomal subunit and is required for its biogenesis... 1 1 1.5% 0.00403 83991
[View Peptides]   TDA9 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 1 1 1.4% 0.00232 141465
[View Peptides]   RAD3 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA poly... 1 1 1.3% 0.00373 89786
[View Peptides]   SAS10 Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of ... 1 1 1.3% 0.00476 70260
[View Peptides]   HER1 Protein of unknown function required for proliferation or remodeling of the ER that is caused by ove... 1 1 1.1% 0.00233 139457
[View Peptides]   VRP1 Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related... 1 1 1.1% 0.00356 82594
[View Peptides]   Reverse_YKR103W NFT1 SGDID:S000001811, Chr XI from 652718-656374, Verified ORF, "Putative transporter of the multidr... 1 1 1.0% 0.00238 137996
[View Peptides]   Reverse_YDR379W RGA2 SGDID:S000002787, Chr IV from 1230157-1233186, Verified ORF, "GTPase-activating protein for the... 1 1 1.0% 0.00288 113291
[View Peptides]   EDE1 Key endocytic protein involved in a network of interactions with other endocytic proteins, binds mem... 1 1 0.8% 0.0021 150783
[View Peptides]   Reverse_YPR095C SYT1 SGDID:S000006299, Chr XVI from 724715-721035, reverse complement, Verified ORF, "Guanine nucleo... 1 1 0.7% 0.00237 137583
[View Peptides]   Reverse_YMR207C HFA1 SGDID:S000004820, Chr XIII from 683563-677192, reverse complement, Verified ORF, "Mitochondrial... 1 1 0.6% 0.00137 241784
[View Peptides]   Reverse_YNR016C ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA car... 1 1 0.5% 0.0013 250351

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle