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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: sample: hx4

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 4 5 48.1% 0.019 10746
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 10 36 36.7% 0.0513 32498
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 4 6 36.4% 0.022 11050
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 10 21 34.9% 0.029 32803
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 5 15 32.3% 0.0279 24668
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 8 14 23.4% 0.0179 36711
[View Peptides]   IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... 4 6 15.0% 0.00534 49976
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 2 3 12.8% 0.00617 21590
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 3 4 12.3% 0.0044 40054
[View Peptides]   VMA6 Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... 2 2 11.6% 0.00234 39791
[View Peptides]   YKT6 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... 2 2 11.0% 0.00403 22707
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 3 3 9.5% 0.0021 64597
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 4 7 9.1% 0.00414 74468
[View Peptides]   CIC1 Essential protein that interacts with proteasome components and has a potential role in proteasome s... 2 2 9.0% 0.00214 42530
[View Peptides]   PIL1 Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... 2 2 8.0% 0.00238 38349
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 3 7 8.0% 0.00498 63004
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 5 7.4% 0.00646 33717
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 4 5 7.1% 0.0024 95529
[View Peptides]   SEC18 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... 3 3 6.6% 0.00159 84056
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 15 6.6% 0.0159 42826
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 2 2 6.4% 0.00161 54545
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 3 3 6.3% 0.00161 84924
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 3 3 5.7% 0.00264 50033
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 3 3 5.5% 0.00179 78344
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 2 2 4.9% 0.00137 64382
[View Peptides]   LYP1 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... 2 3 4.4% 0.00198 68090
[View Peptides]   PEP3 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... 3 3 4.2% 0.00132 107398
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 2 2 4.0% 0.00116 77267
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 4 5 3.9% 0.00107 214849
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 2 2 3.3% 9.27E-4 98712
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 4 5 3.2% 0.00137 166749
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 2 2 3.1% 7.68E-4 122881
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 3 5 3.0% 0.00133 171120
[View Peptides]   VTC3 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 2 2 2.9% 9.65E-4 96553
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 2 2 2.7% 9.01E-4 99490
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 3 4 2.7% 0.001 181660
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198526
[View Peptides]   YER160C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198568
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198658
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198561
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198594
[View Peptides]   YBR012W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198868
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 2.5% 9.18E-4 198838

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