Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 542 | 2073 | 93.7% | 0.156 | 195140 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 21 | 74 | 98.6% | 0.136 | 7729 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 28 | 61 | 65.6% | 0.0212 | 42271 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 7 | 19 | 44.1% | 0.0149 | 18751 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 12 | 29 | 43.1% | 0.0145 | 29280 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 23 | 52 | 56.8% | 0.0144 | 50033 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 21 | 49 | 48.8% | 0.0124 | 54545 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 20 | 35 | 44.4% | 0.00762 | 67400 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 11 | 36 | 15.0% | 0.00617 | 83973 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 5 | 7 | 58.5% | 0.00602 | 16135 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 12 | 23 | 38.1% | 0.00563 | 57749 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 6 | 15 | 25.9% | 0.00528 | 39621 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 10 | 18 | 37.5% | 0.00521 | 46914 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 8 | 13 | 27.5% | 0.00503 | 34465 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 7 | 14 | 27.1% | 0.0049 | 39566 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 19 | 30 | 21.5% | 0.00402 | 111782 |
[ |
SNU13 | RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP invol... | 2 | 4 | 19.0% | 0.00401 | 13569 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 6 | 11 | 24.1% | 0.004 | 36849 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 5 | 10 | 27.9% | 0.00396 | 34805 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 17 | 26 | 37.3% | 0.00386 | 94104 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 12 | 17 | 33.2% | 0.00382 | 61495 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 6 | 9 | 24.3% | 0.00274 | 44738 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 6 | 8 | 31.2% | 0.0027 | 41690 |
[ |
SAM1 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 7 | 8 | 32.7% | 0.00265 | 41818 |
[ |
TPI1 | Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availabi... | 3 | 5 | 26.6% | 0.00255 | 26795 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 5 | 8 | 22.5% | 0.00244 | 45407 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 4 | 9 | 12.7% | 0.00223 | 56956 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 5 | 12 | 14.6% | 0.00212 | 80047 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 3 | 4 | 38.9% | 0.00205 | 27609 |
[ |
RNQ1 | [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate | 4 | 5 | 16.3% | 0.00156 | 42580 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 8 | 10 | 17.8% | 0.0015 | 93289 |
[ |
AHP1 | Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function i... | 2 | 2 | 29.0% | 0.00144 | 19115 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 5 | 6 | 16.2% | 0.00132 | 66087 |
[ |
YHB1 | Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... | 4 | 4 | 19.5% | 0.00127 | 44646 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 4 | 5 | 12.6% | 0.00105 | 65553 |
[ |
HSP42 | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... | 2 | 3 | 10.1% | 0.00101 | 42817 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 7 | 7.0% | 9.64E-4 | 99619 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 5 | 7 | 6.8% | 9.35E-4 | 102172 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 3 | 13.9% | 8.49E-4 | 49800 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 6 | 12 | 5.8% | 8.09E-4 | 218454 |
[ |
BGL2 | Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance | 2 | 2 | 7.3% | 8.08E-4 | 34119 |
[ |
ASP1 | Cytosolic L-asparaginase, involved in asparagine catabolism | 2 | 2 | 11.3% | 6.64E-4 | 41395 |
[ |
ILV1 | Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under genera... | 3 | 3 | 9.4% | 6.59E-4 | 63831 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 2 | 2 | 6.8% | 6.57E-4 | 45384 |
[ |
ACS2 | Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... | 3 | 3 | 7.3% | 5.55E-4 | 75492 |
[ |
NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... | 2 | 2 | 6.7% | 5.02E-4 | 56864 |
[ |
GRS1 | Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... | 2 | 2 | 5.1% | 3.79E-4 | 75411 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198658 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198838 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198526 |
[ |
YGR038C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198520 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198594 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 4 | 4.1% | 2.88E-4 | 198561 |
[ |
FAS2 | Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... | 2 | 2 | 1.7% | 1.34E-4 | 206945 |