Bait Protein: | MIH1 |
Organism: | Saccharomyces cerevisiae |
Publication: | McCusker D, Denison C, Anderson S, Egelhofer TA, Yates JR 3rd, Gygi SP, Kellogg DR. Cdk1 coordinates cell-surface growth with the cell cycle.Nat Cell Biol. 2007 May;9(5):506-15. |
Comments: | experimental1 - mih1-1 |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MIH1 | Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of ... | 16 | 78 | 31.4% | 0.0897 | 63358 |
[ |
KRT10 | keratin 10 | 10 | 59 | 26.3% | 0.0634 | 59519 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 13 | 58 | 37.5% | 0.0594 | 61987 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 9 | 38 | 22.0% | 0.0395 | 66602 |
[ |
Contaminant_KERA... | 11 | 38 | 19.0% | 0.0376 | 65494 | |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 9 | 37 | 22.0% | 0.0384 | 66595 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 14 | 35 | 29.3% | 0.0347 | 69768 |
[ |
KRT2 | keratin 2 | 14 | 33 | 26.2% | 0.0326 | 65865 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 14 | 32 | 31.0% | 0.0319 | 69470 |
[ |
KAP122 | Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucle... | 11 | 32 | 16.7% | 0.0189 | 123531 |
[ |
NAP1 | Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamic... | 8 | 21 | 28.8% | 0.0321 | 47885 |
[ |
HSP60 | Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... | 9 | 21 | 23.4% | 0.0234 | 60752 |
[ |
KRT3 | keratin 3 | 3 | 19 | 2.9% | 0.0192 | 64511 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 3 | 13 | 32.1% | 0.0502 | 17823 |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 4 | 13 | 12.3% | 0.0232 | 39219 |
[ |
contaminant_KERA... | 4 | 13 | 7.8% | 0.0147 | 59822 | |
[ |
KRT5 | keratin 5 | 4 | 13 | 7.1% | 0.014 | 62461 |
[ |
gi|7463016|pir||... | lysyl endopeptidase (EC 3.4.21.50) - Lysobacter enzymogenes | 7 | 12 | 35.3% | 0.0284 | 27961 |
[ |
SPA1_STAAU, cont... | 5 | 12 | 16.0% | 0.0146 | 57320 | |
[ |
SEC21 | Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat... | 9 | 11 | 17.2% | 0.00749 | 104831 |
[ |
SSA3 | ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... | 5 | 11 | 10.6% | 0.0108 | 70547 |
[ |
HSP26 | Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that ... | 4 | 10 | 35.0% | 0.0298 | 23880 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 4 | 10 | 16.9% | 0.0176 | 39062 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 4 | 10 | 16.9% | 0.0176 | 39092 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 1 | 10 | 4.2% | 0.0167 | 42826 |
[ |
contaminant_SPA2... | 2 | 9 | 8.3% | 0.0113 | 55439 | |
[ |
gi|105815 | pir||A34720 keratin 8, type II cytoskeletal - human | 2 | 9 | 7.5% | 0.0119 | 53748 |
[ |
SSA4 | Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational... | 5 | 8 | 11.7% | 0.00794 | 69651 |
[ |
NOT5 | Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcrip... | 3 | 7 | 9.1% | 0.00796 | 65855 |
[ |
RPS5 | Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal ... | 3 | 6 | 17.3% | 0.017 | 25039 |
[ |
CHC1 | Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... | 4 | 6 | 5.1% | 0.00231 | 187233 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 3 | 5 | 12.5% | 0.0102 | 33717 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 4 | 5 | 12.0% | 0.00616 | 57749 |
[ |
IMD3 | Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-... | 2 | 5 | 8.4% | 0.00609 | 56585 |
[ |
IMD2 | Inosine monophosphate dehydrogenase, catalyzes the rate-limiting step in GTP biosynthesis, expressio... | 2 | 5 | 8.4% | 0.00609 | 56530 |
[ |
Contaminant_KERA... | 2 | 5 | 7.0% | 0.00676 | 51531 | |
[ |
RPL14A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 3 | 4 | 21.7% | 0.0185 | 15167 |
[ |
RPL14B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... | 3 | 4 | 21.7% | 0.0185 | 15153 |
[ |
contaminant_KERA... | isb|A33652|A33652|probable keratin 16, type I, pseudogene - human | 2 | 4 | 6.6% | 0.00539 | 50915 |
[ |
INT-STD1, Contam... | 1 | 2 | 4 | 5.1% | 0.0042 | 69271 |
[ |
contaminant_GR78... | 2 | 4 | 3.8% | 0.0039 | 72379 | |
[ |
contaminant_GR78... | 2 | 4 | 3.8% | 0.00383 | 73235 | |
[ |
gi|1143492, gi|8... | pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... | 2 | 4 | 3.8% | 0.0039 | 72116 |
[ |
gi|109893 | pir||A37048 dnaK-type molecular chaperone grp78 precursor - mouse | 2 | 4 | 3.8% | 0.00389 | 72421 |
[ |
GRP78_RAT | 78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1 | 2 | 4 | 3.8% | 0.0039 | 72347 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 3 | 3 | 17.5% | 0.00576 | 35747 |
[ |
PEP4 | Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation o... | 3 | 3 | 12.6% | 0.00472 | 44499 |
[ |
RPS3 | Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... | 2 | 3 | 12.5% | 0.00796 | 26503 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 3 | 3 | 9.6% | 0.00316 | 65553 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 3 | 3 | 8.1% | 0.00292 | 70628 |
[ |
SSE1 | ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... | 3 | 3 | 6.8% | 0.00276 | 77367 |
[ |
RSC8 | Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homol... | 2 | 3 | 4.5% | 0.00343 | 63168 |
[ |
SEC7 | Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of t... | 3 | 3 | 2.8% | 9.51E-4 | 226884 |
[ |
RPL23B, RPL23A | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... | 2 | 2 | 25.5% | 0.0093 | 14473 |
[ |
RPS0A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for mat... | 2 | 2 | 16.3% | 0.00506 | 28024 |
[ |
RPS0B | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for mat... | 2 | 2 | 16.3% | 0.00506 | 27962 |
[ |
MPM1 | Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches | 2 | 2 | 14.3% | 0.00506 | 28470 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 2 | 2 | 10.5% | 0.00384 | 35847 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 2 | 2 | 8.5% | 0.00399 | 34805 |
[ |
RVB2 | Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... | 2 | 2 | 5.5% | 0.0027 | 51612 |
[ |
GSY1 | Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression indu... | 2 | 2 | 5.4% | 0.0018 | 80510 |
[ |
CDC48 | ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p a... | 2 | 2 | 4.1% | 0.00153 | 91996 |
[ |
PHO81 | Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complex... | 2 | 2 | 3.9% | 0.00108 | 134029 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 3.4% | 0.00187 | 74468 |
[ |
STH1 | ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic g... | 2 | 2 | 2.1% | 9.37E-4 | 156742 |
[ |
FAS1 | Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... | 2 | 2 | 1.8% | 6.21E-4 | 228689 |