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Bait Protein: MIH1
Organism: Saccharomyces cerevisiae
Publication: McCusker D, Denison C, Anderson S, Egelhofer TA, Yates JR 3rd, Gygi SP, Kellogg DR. Cdk1 coordinates cell-surface growth with the cell cycle.Nat Cell Biol. 2007 May;9(5):506-15.
Comments: experimental1 - mih1-1

Raw Data:

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 13 58 37.5% 0.0594 61987
[View Peptides]   gi|7463016|pir||... lysyl endopeptidase (EC 3.4.21.50) - Lysobacter enzymogenes 7 12 35.3% 0.0284 27961
[View Peptides]   HSP26 Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that ... 4 10 35.0% 0.0298 23880
[View Peptides]   RPL12B, RPL12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... 3 13 32.1% 0.0502 17823
[View Peptides]   MIH1 Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of ... 16 78 31.4% 0.0897 63358
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 14 32 31.0% 0.0319 69470
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 14 35 29.3% 0.0347 69768
[View Peptides]   NAP1 Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamic... 8 21 28.8% 0.0321 47885
[View Peptides]   KRT10 keratin 10 10 59 26.3% 0.0634 59519
[View Peptides]   KRT2 keratin 2 14 33 26.2% 0.0326 65865
[View Peptides]   RPL23B, RPL23A Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... 2 2 25.5% 0.0093 14473
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 9 21 23.4% 0.0234 60752
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 9 38 22.0% 0.0395 66602
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 9 37 22.0% 0.0384 66595
[View Peptides]   RPL14A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 3 4 21.7% 0.0185 15167
[View Peptides]   RPL14B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... 3 4 21.7% 0.0185 15153
[View Peptides]   Contaminant_KERA... 11 38 19.0% 0.0376 65494
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 3 3 17.5% 0.00576 35747
[View Peptides]   RPS5 Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal ... 3 6 17.3% 0.017 25039
[View Peptides]   SEC21 Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat... 9 11 17.2% 0.00749 104831
[View Peptides]   RPL4B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... 4 10 16.9% 0.0176 39062
[View Peptides]   RPL4A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 4 10 16.9% 0.0176 39092
[View Peptides]   KAP122 Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucle... 11 32 16.7% 0.0189 123531
[View Peptides]   RPS0A Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for mat... 2 2 16.3% 0.00506 28024
[View Peptides]   RPS0B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for mat... 2 2 16.3% 0.00506 27962
[View Peptides]   SPA1_STAAU, cont... 5 12 16.0% 0.0146 57320
[View Peptides]   MPM1 Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches 2 2 14.3% 0.00506 28470
[View Peptides]   PEP4 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation o... 3 3 12.6% 0.00472 44499
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 5 12.5% 0.0102 33717
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 2 3 12.5% 0.00796 26503
[View Peptides]   gi|34069 gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] 4 13 12.3% 0.0232 39219
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 4 5 12.0% 0.00616 57749
[View Peptides]   SSA4 Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational... 5 8 11.7% 0.00794 69651
[View Peptides]   SSA3 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... 5 11 10.6% 0.0108 70547
[View Peptides]   TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... 2 2 10.5% 0.00384 35847
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 3 9.6% 0.00316 65553
[View Peptides]   NOT5 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcrip... 3 7 9.1% 0.00796 65855
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 2 2 8.5% 0.00399 34805
[View Peptides]   IMD3 Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-... 2 5 8.4% 0.00609 56585
[View Peptides]   IMD2 Inosine monophosphate dehydrogenase, catalyzes the rate-limiting step in GTP biosynthesis, expressio... 2 5 8.4% 0.00609 56530
[View Peptides]   contaminant_SPA2... 2 9 8.3% 0.0113 55439
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 3 3 8.1% 0.00292 70628
[View Peptides]   contaminant_KERA... 4 13 7.8% 0.0147 59822
[View Peptides]   gi|105815 pir||A34720 keratin 8, type II cytoskeletal - human 2 9 7.5% 0.0119 53748
[View Peptides]   KRT5 keratin 5 4 13 7.1% 0.014 62461
[View Peptides]   Contaminant_KERA... 2 5 7.0% 0.00676 51531
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 3 3 6.8% 0.00276 77367
[View Peptides]   contaminant_KERA... isb|A33652|A33652|probable keratin 16, type I, pseudogene - human 2 4 6.6% 0.00539 50915
[View Peptides]   RVB2 Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... 2 2 5.5% 0.0027 51612
[View Peptides]   GSY1 Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression indu... 2 2 5.4% 0.0018 80510
[View Peptides]   CHC1 Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... 4 6 5.1% 0.00231 187233
[View Peptides]   INT-STD1, Contam... 1 2 4 5.1% 0.0042 69271
[View Peptides]   RSC8 Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homol... 2 3 4.5% 0.00343 63168
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 1 10 4.2% 0.0167 42826
[View Peptides]   CDC48 ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p a... 2 2 4.1% 0.00153 91996
[View Peptides]   PHO81 Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complex... 2 2 3.9% 0.00108 134029
[View Peptides]   contaminant_GR78... 2 4 3.8% 0.0039 72379
[View Peptides]   contaminant_GR78... 2 4 3.8% 0.00383 73235
[View Peptides]   gi|1143492, gi|8... pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... 2 4 3.8% 0.0039 72116
[View Peptides]   gi|109893 pir||A37048 dnaK-type molecular chaperone grp78 precursor - mouse 2 4 3.8% 0.00389 72421
[View Peptides]   GRP78_RAT 78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1 2 4 3.8% 0.0039 72347
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 2 3.4% 0.00187 74468
[View Peptides]   KRT3 keratin 3 3 19 2.9% 0.0192 64511
[View Peptides]   SEC7 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of t... 3 3 2.8% 9.51E-4 226884
[View Peptides]   STH1 ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic g... 2 2 2.1% 9.37E-4 156742
[View Peptides]   FAS1 Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... 2 2 1.8% 6.21E-4 228689

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