Protein Overview: SIL1

General Information:
Name(s) found:	SIL1 / YOL031C [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein [SGD]
Organism:	Saccharomyces cerevisiae
Length:	421 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum [IPI]
Biological Process:	SRP-dependent cotranslational protein targeting to membrane, translocation [IGI][IMP]
Molecular Function:	adenyl-nucleotide exchange factor activity [IDA][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVRILPIILS ALSSKLVAST ILHSSIHSVP SGGEIISAED LKELEISGNS ICVDNRCYPK  60
   61 IFEPRHDWQP ILPGQELPGG LDIRINMDTG LKEAKLNDEK NVGDNGSHEL IVSSEDMKAS 120
  121 PGDYEFSSDF KEMRNIIDSN PTLSSQDIAR LEDSFDRIME FAHDYKHGYK IITHEFALLA 180
  181 NLSLNENLPL TLRELSTRVI TSCLRNNPPV VEFINESFPN FKSKIMAALS NLNDSNHRSS 240
  241 NILIKRYLSI LNELPVTSED LPIYSTVVLQ NVYERNNKDK QLQIKVLELI SKILKADMYE 300
  301 NDDTNLILFK RNAENWSSNL QEWANEFQEM VQNKSIDELH TRTFFDTLYN LKKIFKSDIT 360
  361 INKGFLNWLA QQCKARQSNL DNGLQERDTE QDSFDKKLID SRHLIFGNPM AHRIKNFRDE 420
  421 L

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		KEX2 SIL1
View Details	Krogan NJ, et al. (2006)		KEX2 SIL1
View Details	Gavin AC, et al. (2006)		KEX2 SIL1 YBT1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SIL1	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
protein transporter activity	2.17038865096656	bayes_pls_golite062009
protein binding	2.0097220759196	bayes_pls_golite062009
binding	1.83766472499136	bayes_pls_golite062009
transporter activity	1.12250209220616	bayes_pls_golite062009
substrate-specific transporter activity	0.68280791022469	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.3757449438966	bayes_pls_golite062009
nucleic acid binding	0.371883653230174	bayes_pls_golite062009
protein transmembrane transporter activity	0.347277736469816	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.298901699355651	bayes_pls_golite062009
signal sequence binding	0.279693431859311	bayes_pls_golite062009
nuclear localization sequence binding	0.241630311762028	bayes_pls_golite062009
hydrolase activity	0.0962597719675237	bayes_pls_golite062009

No functions predicted.

Source: Reynolds et al. 2008. Manuscript submitted

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..125]	deduced	N/A	No confident structure predictions are available.
2	View Details	[126..231]	ORFEUS	9.99	Importin alpha
3	View Details	[232..324]	ORFEUS	9.99	Importin alpha
4	View Details	[325..421]	ORFEUS	9.99	Importin alpha

Protein Overview: SIL1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: