YRC Public Data Repository - Protein Structure Prediction

Protein:	RLUC_ECOLI
Organism:	Escherichia coli
Length:	319 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RLUC_ECOLI.

Description	E-value	Query Range	Subject Range
RLUC_ECOL6 - Ribosomal large subunit pseudouridine synthase C OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700... gi\|227836761, gi... - gi\|227886557\|ref\|ZP_04004362.1\| 23S rRNA pseudouridylate synthase C [Escherichia coli 83972], gi\|227... RLUC_ECOL6 - Ribosomal large subunit pseudouridine synthase C OS=Escherichia coli O6 GN=rluC PE=3 SV=1	318.0	[0..1]	[319..1]
gi\|74311647, gi\|... - gi\|74311647\|ref\|YP_310066.1\| 23S rRNA pseudouridylate synthase C [Shigella sonnei Ss046], gi\|7385512...	317.0	[0..1]	[319..1]
gi\|12514630, gi\|... - gi\|15801203\|ref\|NP_287220.1\| 23S rRNA pseudouridylate synthase C [Escherichia coli O157:H7 EDL933], ... gi\|209773284, gi... - pir\|\|D85671 hypothetical protein yceC [imported] - Escherichia coli (strain O157:H7, substrain EDL9... RLUC_ECO57 - Ribosomal large subunit pseudouridine synthase C OS=Escherichia coli O157:H7 GN=rluC PE=3 SV=1 gi\|208816352, gi... - gi\|208816352\|ref\|ZP_03257531.1\| ribosomal large subunit pseudouridine synthase C [Escherichia coli O... gi\|254591868, gi... - gi\|254792468\|ref\|YP_003077305.1\| Ribosomal large subunit pseudouridine synthase C [Escherichia coli ... gi\|209400210, gi... - gi\|209400210\|ref\|YP_002269931.1\| ribosomal large subunit pseudouridine synthase C [Escherichia coli ... gi\|217328324, gi... - gi\|217328324\|ref\|ZP_03444406.1\| ribosomal large subunit pseudouridine synthase C [Escherichia coli O... gi\|218704495, gi... - gi\|218704495\|ref\|YP_002412014.1\| 23S rRNA pseudouridylate synthase C [Escherichia coli UMN026], gi\|2... gi\|195935476 - gi\|195935476\|ref\|ZP_03080858.1\| 23S rRNA pseudouridylate synthase C [Escherichia coli O157:H7 str. E... gi\|15830718, gi\|... - gi\|15830718\|ref\|NP_309491.1\| 23S rRNA pseudouridylate synthase C [Escherichia coli O157:H7 str. Saka... gi\|208807624, gi... - gi\|208807624\|ref\|ZP_03249961.1\| ribosomal large subunit pseudouridine synthase C [Escherichia coli O...	317.0	[0..1]	[319..1]
gi\|81241450, gi\|... - gi\|82777302\|ref\|YP_403651.1\| 23S rRNA pseudouridylate synthase C [Shigella dysenteriae Sd197], gi\|81...	316.0	[0..1]	[319..1]
gi\|229330881, gi... - gi\|229498191\|ref\|ZP_04391888.1\| pseudouridine synthase, RluA family [Geobacillus sp. Y412MC52], gi\|2... gi\|56379523, gi\|... - gi\|56419681\|ref\|YP_146999.1\| hypothetical protein GK1146 [Geobacillus kaustophilus HTA426], gi\|56379... gi\|221087579, gi... - gi\|221087579\|ref\|ZP_03557116.1\| pseudouridine synthase, RluA family [Geobacillus sp. Y412MC61], gi\|2...	316.0	[0..7]	[318..2]
RLUC_SALTY - Ribosomal large subunit pseudouridine synthase C OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ... gi\|206709109, gi... - gi\|207857306\|ref\|YP_002243957.1\| 23S rRNA pseudouridylate synthase C [Salmonella enterica subsp. ent... gi\|194448791, gi... - gi\|194448791\|ref\|YP_002045185.1\| 23S rRNA pseudouridylate synthase C [Salmonella enterica subsp. ent... RLUC_SALTY - Ribosomal large subunit pseudouridine synthase C OS=Salmonella typhimurium GN=rluC PE=3 SV=1 gi\|194444187, gi... - gi\|194444187\|ref\|YP_002040440.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enteri... gi\|168822633, gi... - gi\|205340990\|gb\|EDZ27754.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enterica su... gi\|205336408, gi... - gi\|205336408\|gb\|EDZ23172.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enterica su... gi\|204320190, gi... - gi\|204930731\|ref\|ZP_03221604.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enteric... gi\|198244450, gi... - gi\|198244450\|ref\|YP_002215951.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enteri... gi\|197242045, gi... - gi\|197263790\|ref\|ZP_03163864.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enteric... gi\|194738103, gi... - gi\|194738103\|ref\|YP_002114185.1\| ribosomal large subunit pseudouridine synthase C [Salmonella enteri... RLUC_SALTY - Ribosomal large subunit pseudouridine synthase C OS=Salmonella typhimurium GN=rluC PE=3 SV=1 gi\|197289855, gi... - gi\|197289855\|gb\|EDY29216.1\| ribosomal large subunit pseudouridine synthase C (rRNA-uridine isomerase... lcl\|NC_016856.1_... - [gene=rluC] [protein=23S rRNA pseudouridylate synthase C] [protein_id=YP_005236942.2] [location=1230... gi\|238910994 - gi\|238910994\|ref\|ZP_04654831.1\| 23S rRNA pseudouridylate synthase C [Salmonella enterica subsp. ente...	315.0	[0..1]	[319..1]
gi\|187732109, gi... - gi\|187732109\|ref\|YP_001880745.1\| ribosomal large subunit pseudouridine synthase C [Shigella boydii C... gi\|81245859, gi\|... - gi\|82544448\|ref\|YP_408395.1\| 23S rRNA pseudouridylate synthase C [Shigella boydii Sb227], gi\|8124585... gi\|110614649, gi... - gi\|110805101\|ref\|YP_688621.1\| 23S rRNA pseudouridylate synthase C [Shigella flexneri 5 str. 8401], g...	314.0	[0..1]	[319..1]
gi\|20807974, gi\|... - gi\|20807974\|ref\|NP_623145.1\| pseudouridylate synthase, 23S RNA-specific [Thermoanaerobacter tengcong... gi\|20807974 - gi\|20807974\|ref\|NP_623145.1\| Pseudouridylate synthase, 23S RNA-specific [Thermoanaerobacter tengcong...	314.0	[0..14]	[319..12]
gi\|15615105, gi\|... - gi\|15615105\|ref\|NP_243408.1\| hypothetical protein BH2542 [Bacillus halodurans C-125], gi\|10175162\|db... gi\|25322336 - pir\|\|F83967 hypothetical protein BH2542 [imported] - Bacillus halodurans (strain C-125)	313.0	[0..1]	[319..1]
gi\|62127337, gi\|... - gi\|62179704\|ref\|YP_216121.1\| 23S rRNA pseudouridylate synthase [Salmonella enterica subsp. enterica ...	312.0	[0..1]	[319..29]

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Predicted Domain #1

Region A:
Residues: [1-79]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN KKRIKPEYKL  60
   61 EAGDEVRIPP VRVAEREEE

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	3.03
Match:	1qyuA
Description:	Structure of the catalytic domain of 23S rRNA pseudouridine synthase RluD
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
pseudouridine synthase activity	4.91262436325473	bayes_pls_golite062009
intramolecular transferase activity	4.03380803526801	bayes_pls_golite062009
isomerase activity	2.9653842461049	bayes_pls_golite062009
catalytic activity	2.2317719900989	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [80-319]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AVSPHLQKVA ALADVILYED DHILVLNKPS GTAVHGGSGL SFGVIEGLRA LRPEARFLEL  60
   61 VHRLDRDTSG VLLVAKKRSA LRSLHEQLRE KGMQKDYLAL VRGQWQSHVK SVQAPLLKNI 120
  121 LQSGERIVRV SQEGKPSETR FKVEERYAFA TLVRCSPVTG RTHQIRVHTQ YAGHPIAFDD 180
  181 RYGDREFDRQ LTEAGTGLNR LFLHAAALKF THPGTGEVMR IEAPMDEGLK RCLQKLRNAR 240
  241

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	64.0
Match:	1xpiA
Description:	Crystal structure of the catalytic domain of E. coli pseudouridine synthase RluC
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: