YRC Public Data Repository - Protein Structure Prediction

Protein:	PFLB_ECOLI
Organism:	Escherichia coli
Length:	759 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PFLB_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|32033949 - gi\|32033949\|ref\|ZP_00134205.1\| COG1882: Pyruvate-formate lyase [Actinobacillus pleuropneumoniae sero... gi\|126097302, gi... - gi\|126208510\|ref\|YP_001053735.1\| formate acetyltransferase [Actinobacillus pleuropneumoniae L20], gi... gi\|190150361, gi... - gi\|190150361\|ref\|YP_001968886.1\| formate acetyltransferase [Actinobacillus pleuropneumoniae serovar ...	1174.0	[0..1]	[759..2]
gi\|75229892, gi\|... - gi\|75229892\|ref\|ZP_00716415.1\| COG1882: Pyruvate-formate lyase [Escherichia coli B7A], gi\|191166987\|...	1172.0	[0..1]	[759..2]
PFLB_ECOLI - Formate acetyltransferase 1 (EC 2.3.1.54) (Pyruvate formate-lyase 1) - Escherichia coli EG10701 - formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1 / Formate C-acetyltransferase 1; ... gi\|218364577, gi... - gi\|218557808\|ref\|YP_002390721.1\| pyruvate formate lyase I [Escherichia coli S88], gi\|218364577\|emb\|C... gi\|218553489, gi... - gi\|218553489\|ref\|YP_002386402.1\| pyruvate formate lyase I [Escherichia coli IAI1], gi\|218360257\|emb\|... gi\|218426307, gi... - gi\|218688743\|ref\|YP_002396955.1\| pyruvate formate lyase I [Escherichia coli ED1a], gi\|218426307\|emb\|... gi\|75512059, gi\|... - gi\|75512059\|ref\|ZP_00734644.1\| COG1882: Pyruvate-formate lyase [Escherichia coli 53638], gi\|18849291... gi\|73854936, gi\|... - gi\|74311459\|ref\|YP_309878.1\| formate acetyltransferase 1 [Shigella sonnei Ss046], gi\|73854936\|gb\|AAZ... gi\|191172132, gi... - gi\|75240977\|ref\|ZP_00724866.1\| COG1882: Pyruvate-formate lyase [Escherichia coli F11], gi\|191172132\|... gi\|192927747, gi... - gi\|75257382\|ref\|ZP_00728898.1\| COG1882: Pyruvate-formate lyase [Escherichia coli E22], gi\|193064690\|... gi\|117623121, gi... - gi\|117623121\|ref\|YP_852034.1\| PflB [Escherichia coli APEC O1], gi\|115512245\|gb\|ABJ00320.1\| PflB [Esc... gi\|124531972, gi... - gi\|124531972\|ref\|ZP_01702031.1\| formate acetyltransferase [Escherichia coli B], gi\|124498234\|gb\|EAY4... gi\|157160425, gi... - gi\|75195091\|ref\|ZP_00705161.1\| COG1882: Pyruvate-formate lyase [Escherichia coli HS], gi\|157160425\|r... gi\|194413580, gi... - gi\|75210408\|ref\|ZP_00710564.1\| COG1882: Pyruvate-formate lyase [Escherichia coli B171], gi\|194428365... gi\|194438693, gi... - gi\|83585846\|ref\|ZP_00924486.1\| COG1882: Pyruvate-formate lyase [Escherichia coli 101-1], gi\|19443869... gi\|75189958, gi\|... - gi\|75189958\|ref\|ZP_00703225.1\| COG1882: Pyruvate-formate lyase [Escherichia coli E24377A], gi\|157157... gi\|89107753, gi\|... - gi\|89107753\|ref\|AP_001533.1\| pyruvate formate lyase I [Escherichia coli str. K-12 substr. W3110], gi... gi\|91071578, gi\|... - gi\|91210004\|ref\|YP_539990.1\| formate acetyltransferase 1 [Escherichia coli UTI89], gi\|91071578\|gb\|AB... EG10701 - formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1 / Formate C-acetyltransferase 1; ... gi\|110641100, gi... - gi\|110641100\|ref\|YP_668830.1\| formate acetyltransferase 1 [Escherichia coli 536], gi\|110342692\|gb\|AB... gi\|227838884, gi... - gi\|227884132\|ref\|ZP_04001937.1\| pyruvate formate lyase I [Escherichia coli 83972], gi\|227838884\|gb\|E... gi\|237707109, gi... - gi\|237707109\|ref\|ZP_04537590.1\| formate acetyltransferase 1 [Escherichia sp. 3_2_53FAA], gi\|22689831... gi\|238900161, gi... - gi\|238900161\|ref\|YP_002925957.1\| pyruvate formate lyase I [Escherichia coli BW2952], gi\|238863359\|gb... gi\|169888396, gi... - gi\|170080561\|ref\|YP_001729881.1\| pyruvate formate lyase I [Escherichia coli str. K-12 substr. DH10B]... gi\|242376718, gi... - gi\|251784445\|ref\|YP_002998749.1\| hypothetical protein B21_00914 [Escherichia coli BL21], gi\|24237671... gi\|169755623, gi... - gi\|170020695\|ref\|YP_001725649.1\| formate acetyltransferase [Escherichia coli ATCC 8739], gi\|16975562... gi\|253977132, gi... - gi\|254287825\|ref\|YP_003053573.1\| pyruvate formate lyase I [Escherichia coli BL21(DE3)], gi\|253977132... gi\|253972918, gi... - gi\|254161017\|ref\|YP_003044125.1\| pyruvate formate lyase I [Escherichia coli B str. REL606], gi\|25397...	1172.0	[0..1]	[759..2]
gi\|187430216, gi... - gi\|75178294\|ref\|ZP_00698348.1\| COG1882: Pyruvate-formate lyase [Shigella boydii BS512], gi\|187733224... gi\|81246052, gi\|... - gi\|82544641\|ref\|YP_408588.1\| formate acetyltransferase 1 [Shigella boydii Sb227], gi\|81246052\|gb\|ABB... gi\|215264100, gi... - gi\|215486028\|ref\|YP_002328459.1\| pyruvate formate lyase I [Escherichia coli O127:H6 str. E2348/69], ...	1171.0	[0..1]	[759..2]
gi\|194443827, gi... - gi\|194443827\|ref\|YP_002040171.1\| formate acetyltransferase [Salmonella enterica subsp. enterica sero... gi\|194410131, gi... - gi\|194451827\|ref\|YP_002044965.1\| formate acetyltransferase [Salmonella enterica subsp. enterica sero... gi\|194471684, gi... - gi\|194471684\|ref\|ZP_03077668.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serov... gi\|197213430, gi... - gi\|197249727\|ref\|YP_002145890.1\| formate acetyltransferase [Salmonella enterica subsp. enterica sero... gi\|205339897, gi... - gi\|205339897\|gb\|EDZ26661.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar H... gi\|168263538, gi... - gi\|205347666\|gb\|EDZ34297.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar H... gi\|168466561, gi... - gi\|195630994\|gb\|EDX49580.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar N... gi\|205340731, gi... - gi\|205340731\|gb\|EDZ27495.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar W... gi\|161364157, gi... - gi\|161614793\|ref\|YP_001588758.1\| hypothetical protein SPAB_02545 [Salmonella enterica subsp. enteric... gi\|167550352, gi... - gi\|205324590\|gb\|EDZ12429.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar S... gi\|205327469, gi... - gi\|205327469\|gb\|EDZ14233.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar 4... gi\|168230868, gi... - gi\|205334836\|gb\|EDZ21600.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serovar K... gi\|206708167, gi... - gi\|207856364\|ref\|YP_002243015.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica se... gi\|213424590 - gi\|213424590\|ref\|ZP_03357373.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica ser... gi\|213648475 - gi\|213648475\|ref\|ZP_03378528.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica ser... gi\|214002575 - gi\|214002575\|ref\|ZP_03413310.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica ser... gi\|197264023, gi... - gi\|197264023\|ref\|ZP_03164097.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serov... gi\|197362900, gi... - gi\|197362900\|ref\|YP_002142537.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica se... gi\|197936423, gi... - gi\|198241907\|ref\|YP_002214896.1\| formate acetyltransferase [Salmonella enterica subsp. enterica sero... gi\|199603024, gi... - gi\|200390579\|ref\|ZP_03217190.1\| formate acetyltransferase [Salmonella enterica subsp. enterica serov... gi\|56413977, gi\|... - gi\|56413977\|ref\|YP_151052.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica serova... gi\|16764333, gi\|... - gi\|16764333\|ref\|NP_459948.1\| pyruvate formate lyase I [Salmonella typhimurium LT2], gi\|16419484\|gb\|A... gi\|238913254 - gi\|238913254\|ref\|ZP_04657091.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica ser... gi\|29138014, gi\|... - gi\|29142384\|ref\|NP_805726.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica serova... gi\|16502136, gi\|... - gi\|25285937\|pir\|\|AC0613 formate acetyltransferase 1 [imported] - Salmonella enterica subsp. enterica... lcl\|NC_016856.1_... - [gene=pflB] [protein=pyruvate formate lyase I] [protein_id=YP_005236689.1] [location=complement(1013... gi\|16759843 - gi\|16759843\|ref\|NP_455460.1\| formate acetyltransferase 1 [Salmonella enterica subsp. enterica serova...	1171.0	[0..1]	[759..2]
gi\|24112311, gi\|... - gi\|24112311\|ref\|NP_706821.1\| formate acetyltransferase 1 [Shigella flexneri 2a str. 301], gi\|2405116... gi\|110804911, gi... - gi\|110804911\|ref\|YP_688431.1\| formate acetyltransferase 1 [Shigella flexneri 5 str. 8401], gi\|110614... gi\|30040684, gi\|... - gi\|30062438\|ref\|NP_836609.1\| formate acetyltransferase 1 [Shigella flexneri 2a str. 2457T], gi\|30040...	1170.0	[0..1]	[759..2]
gi\|62127130, gi\|... - gi\|62179497\|ref\|YP_215914.1\| pyruvate formate lyase I, induced anaerobically [Salmonella enterica su...	1169.0	[0..1]	[759..2]
gi\|83569142, gi\|... - gi\|83569142\|ref\|ZP_00920601.1\| COG1882: Pyruvate-formate lyase [Shigella dysenteriae 1012], gi\|19443...	1169.0	[0..1]	[759..2]
gi\|25285928, gi\|... - pir\|\|H85615 formate acetyltransferase 1 [imported] - Escherichia coli (strain O157:H7, substrain ED... gi\|254792102, gi... - gi\|254792102\|ref\|YP_003076939.1\| pyruvate formate lyase I [Escherichia coli O157:H7 str. TW14359], g... gi\|12514066, gi\|... - gi\|15800764\|ref\|NP_286778.1\| formate acetyltransferase 1 [Escherichia coli O157:H7 EDL933], gi\|12514... gi\|13360445, gi\|... - gi\|15830240\|ref\|NP_309013.1\| formate acetyltransferase 1 [Escherichia coli O157:H7], gi\|13360445\|dbj... gi\|217324930, gi... - gi\|217324930\|ref\|ZP_03441014.1\| formate acetyltransferase [Escherichia coli O157:H7 str. TW14588], g... gi\|209399377, gi... - gi\|209399377\|ref\|YP_002269575.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4115], g... gi\|218370565, gi... - gi\|218700579\|ref\|YP_002408208.1\| pyruvate formate lyase I [Escherichia coli IAI39], gi\|218370565\|emb... gi\|218694376, gi... - gi\|218694376\|ref\|YP_002402043.1\| pyruvate formate lyase I [Escherichia coli 55989], gi\|218351108\|emb... gi\|195939564 - gi\|195939564\|ref\|ZP_03084946.1\| formate acetyltransferase 1 [Escherichia coli O157:H7 str. EC4024] gi\|170681780, gi... - gi\|170681780\|ref\|YP_001744268.1\| formate acetyltransferase [Escherichia coli SMS-3-5], gi\|170519498\|... gi\|208737573, gi... - gi\|208822088\|ref\|ZP_03262407.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4042], gi... gi\|208732552, gi... - gi\|208815904\|ref\|ZP_03257083.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4045], gi... gi\|168783814, gi... - gi\|188998916\|gb\|EDU67902.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4076], gi\|168... gi\|187768347, gi... - gi\|187768347\|gb\|EDU32191.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4196], gi\|168... gi\|189376449, gi... - gi\|189376449\|gb\|EDU94865.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC508], gi\|1688... gi\|189372706, gi... - gi\|189372706\|gb\|EDU91122.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC869], gi\|1687... gi\|189355903, gi... - gi\|189355903\|gb\|EDU74322.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4401], gi\|168... gi\|168752114, gi... - gi\|188013955\|gb\|EDU52077.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4113], gi\|168... gi\|13360445, gi\|... - gi\|15830240\|ref\|NP_309013.1\| formate acetyltransferase 1 [Escherichia coli O157:H7 str. Sakai], gi\|1... gi\|189361112, gi... - gi\|189361112\|gb\|EDU79531.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4486], gi\|168... gi\|81241725, gi\|... - gi\|82777577\|ref\|YP_403926.1\| formate acetyltransferase 1 [Shigella dysenteriae Sd197], gi\|81241725\|g... gi\|168762962, gi... - gi\|189366827\|gb\|EDU85243.1\| formate acetyltransferase [Escherichia coli O157:H7 str. EC4501], gi\|168...	1169.0	[0..1]	[759..2]
gi\|75234414, gi\|... - gi\|75234414\|ref\|ZP_00718775.1\| COG1882: Pyruvate-formate lyase [Escherichia coli E110019], gi\|193070...	1168.0	[0..1]	[759..2]

Back

Predicted Domain #1

Region A:
Residues: [1-759]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SELNEKLATA WEGFTKGDWQ NEVNVRDFIQ KNYTPYEGDE SFLAGATEAT TTLWDKVMEG  60
   61 VKLENRTHAP VDFDTAVAST ITSHDAGYIN KQLEKIVGLQ TEAPLKRALI PFGGIKMIEG 120
  121 SCKAYNRELD PMIKKIFTEY RKTHNQGVFD VYTPDILRCR KSGVLTGLPD AYGRGRIIGD 180
  181 YRRVALYGID YLMKDKLAQF TSLQADLENG VNLEQTIRLR EEIAEQHRAL GQMKEMAAKY 240
  241 GYDISGPATN AQEAIQWTYF GYLAAVKSQN GAAMSFGRTS TFLDVYIERD LKAGKITEQE 300
  301 AQEMVDHLVM KLRMVRFLRT PEYDELFSGD PIWATESIGG MGLDGRTLVT KNSFRFLNTL 360
  361 YTMGPSPEPN MTILWSEKLP LNFKKFAAKV SIDTSSLQYE NDDLMRPDFN NDDYAIACCV 420
  421 SPMIVGKQMQ FFGARANLAK TMLYAINGGV DEKLKMQVGP KSEPIKGDVL NYDEVMERMD 480
  481 HFMDWLAKQY ITALNIIHYM HDKYSYEASL MALHDRDVIR TMACGIAGLS VAADSLSAIK 540
  541 YAKVKPIRDE DGLAIDFEIE GEYPQFGNND PRVDDLAVDL VERFMKKIQK LHTYRDAIPT 600
  601 QSVLTITSNV VYGKKTGNTP DGRRAGAPFG PGANPMHGRD QKGAVASLTS VAKLPFAYAK 660
  661 DGISYTFSIV PNALGKDDEV RKTNLAGLMD GYFHHEASIE GGQHLNVNVM NREMLLDAME 720
  721 NPEKYPQLTI RVSGYAVRFN SLTKEQQQDV ITRTFTQSM

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1000.0
Match:	1h16A
Description:	Pyruvate formate-lyase, PFL
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	1.95058695475279	bayes_pls_golite062009
oxidoreductase activity, acting on CH or CH2 groups, disulfide as acceptor	1.72563679072631	bayes_pls_golite062009
oxidoreductase activity, acting on CH or CH2 groups	1.43559439297918	bayes_pls_golite062009
oxidoreductase activity	1.42705638794743	bayes_pls_golite062009
ribonucleoside-diphosphate reductase activity	1.37298861502572	bayes_pls_golite062009
C-acyltransferase activity	0.0923196663496405	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: