YRC Public Data Repository - Protein Structure Prediction

Protein:	CUSA_ECOLI
Organism:	Escherichia coli
Length:	1047 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for CUSA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|75229208, gi\|... - gi\|75229208\|ref\|ZP_00715776.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli B7A], gi\|1911... gi\|209919942, gi... - gi\|209919942\|ref\|YP_002294026.1\| aminoglycoside/multidrug efflux system [Escherichia coli SE11], gi\|...	783.0	[0..5]	[1047..1]
gi\|157080537, gi... - gi\|75187214\|ref\|ZP_00700481.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli E24377A], gi\|...	781.0	[0..5]	[1047..1]
gi\|191172943, gi... - gi\|75240119\|ref\|ZP_00724078.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli F11], gi\|1911...	781.0	[0..5]	[1047..1]
gi\|192958289, gi... - gi\|75237787\|ref\|ZP_00721803.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli E110019], gi\|...	781.0	[0..5]	[1047..1]
gi\|218559405, gi... - gi\|218559405\|ref\|YP_002392318.1\| aminoglycoside/multidrug efflux system [Escherichia coli S88], gi\|2... gi\|91211801, gi\|... - gi\|91211801\|ref\|YP_541787.1\| aminoglycoside/multidrug efflux system [Escherichia coli UTI89], gi\|910... gi\|218696096, gi... - gi\|218696096\|ref\|YP_002403763.1\| aminoglycoside/multidrug efflux system [Escherichia coli 55989], gi... gi\|226901351, gi... - gi\|237704985\|ref\|ZP_04535466.1\| aminoglycoside/multidrug efflux system [Escherichia sp. 3_2_53FAA], ... gi\|218361761, gi... - gi\|218554993\|ref\|YP_002387906.1\| aminoglycoside/multidrug efflux system [Escherichia coli IAI1], gi\|... gi\|218700926, gi... - gi\|218700926\|ref\|YP_002408555.1\| aminoglycoside/multidrug efflux system [Escherichia coli IAI39], gi... gi\|192927659, gi... - gi\|75257273\|ref\|ZP_00728797.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli E22], gi\|1930... gi\|115513790, gi... - gi\|117624666\|ref\|YP_853579.1\| aminoglycoside/multidrug efflux system [Escherichia coli APEC O1], gi\|...	781.0	[0..5]	[1047..1]
gi\|26109245, gi\|... - gi\|26248839\|ref\|NP_754879.1\| aminoglycoside/multidrug efflux system [Escherichia coli CFT073], gi\|26...	781.0	[0..5]	[1047..1]
gi\|24113798, gi\|... - gi\|24113798\|ref\|NP_708308.1\| aminoglycoside/multidrug efflux system [Shigella flexneri 2a str. 301],... gi\|30042104, gi\|... - gi\|30063849\|ref\|NP_838020.1\| aminoglycoside/multidrug efflux system [Shigella flexneri 2a str. 2457T...	781.0	[0..5]	[1047..1]
ACRD_ECOLI - Probable aminoglycoside efflux pump (Acriflavine resistance protein D) - Escherichia coli gi\|238901633, gi... - gi\|238901633\|ref\|YP_002927429.1\| aminoglycoside/multidrug efflux system [Escherichia coli BW2952], g... gi\|242378069, gi... - gi\|251785796\|ref\|YP_003000100.1\| hypothetical protein B21_02323 [Escherichia coli BL21], gi\|24237806... gi\|169889914, gi... - gi\|170082079\|ref\|YP_001731399.1\| aminoglycoside/multidrug efflux system [Escherichia coli str. K-12 ... gi\|254289205, gi... - gi\|254289205\|ref\|YP_003054953.1\| aminoglycoside/multidrug efflux system [Escherichia coli BL21(DE3)]... gi\|124499642, gi... - gi\|124529782\|ref\|ZP_01700659.1\| transporter, hydrophobe/amphiphile efflux-1 (HAE1) family [Escherich... gi\|169754174, gi... - gi\|170019246\|ref\|YP_001724200.1\| aminoglycoside/multidrug efflux system [Escherichia coli ATCC 8739]... gi\|253974345, gi... - gi\|254162444\|ref\|YP_003045552.1\| aminoglycoside/multidrug efflux system [Escherichia coli B str. REL... EG10014 - aminoglycoside /multidrug efflux system / Aminoglycoside efflux pump; RND-type transporter gi\|89109275, gi\|... - gi\|89109275\|ref\|AP_003055.1\| aminoglycoside/multidrug efflux system [Escherichia coli str. K-12 subs... EG10014 - aminoglycoside /multidrug efflux system / Aminoglycoside efflux pump; RND-type transporter	780.0	[0..5]	[1047..1]
gi\|75196475, gi\|... - gi\|75196475\|ref\|ZP_00706545.1\| COG0841: Cation/multidrug efflux pump [Escherichia coli HS], gi\|15716...	780.0	[0..5]	[1047..1]
gi\|74312995, gi\|... - gi\|74312995\|ref\|YP_311414.1\| aminoglycoside/multidrug efflux system [Shigella sonnei Ss046], gi\|7385...	780.0	[0..5]	[1047..1]

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Predicted Domain #1

Region A:
Residues: [1-1047]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIEWIIRRSV ANRFLVLMGA LFLSIWGTWT IINTPVDALP DLSDVQVIIK TSYPGQAPQI  60
   61 VENQVTYPLT TTMLSVPGAK TVRGFSQFGD SYVYVIFEDG TDPYWARSRV LEYLNQVQGK 120
  121 LPAGVSAELG PDATGVGWIY EYALVDRSGK HDLADLRSLQ DWFLKYELKT IPDVAEVASV 180
  181 GGVVKEYQVV IDPQRLAQYG ISLAEVKSAL DASNQEAGGS SIELAEAEYM VRASGYLQTL 240
  241 DDFNHIVLKA SENGVPVYLR DVAKVQIGPE MRRGIAELNG EGEVAGGVVI LRSGKNAREV 300
  301 IAAVKDKLET LKSSLPEGVE IVTTYDRSQL IDRAIDNLSG KLLEEFIVVA VVCALFLWHV 360
  361 RSALVAIISL PLGLCIAFIV MHFQGLNANI MSLGGIAIAV GAMVDAAIVM IENAHKRLEE 420
  421 WQHQHPDATL DNKTRWQVIT DASVEVGPAL FISLLIITLS FIPIFTLEGQ EGRLFGPLAF 480
  481 TKTYAMAGAA LLAIVVIPIL MGYWIRGKIP PESSNPLNRF LIRVYHPLLL KVLHWPKTTL 540
  541 LVAALSVLTV LWPLNKVGGE FLPQINEGDL LYMPSTLPGI SAAEAASMLQ KTDKLIMSVP 600
  601 EVARVFGKTG KAETATDSAP LEMVETTIQL KPQEQWRPGM TMDKIIEELD NTVRLPGLAN 660
  661 LWVPPIRNRI DMLSTGIKSP IGIKVSGTVL ADIDAMAEQI EEVARTVPGV ASALAERLEG 720
  721 GRYINVEINR EKAARYGMTV ADVQLFVTSA VGGAMVGETV EGIARYPINL RYPQSWRDSP 780
  781 QALRQLPILT PMKQQITLAD VADIKVSTGP SMLKTENARP TSWIYIDARD RDMVSVVHDL 840
  841 QKAIAEKVQL KPGTSVAFSG QFELLERANH KLKLMVPMTL MIIFVLLYLA FRRVGEALLI 900
  901 ISSVPFALVG GIWLLWWMGF HLSVATGTGF IALAGVAAEF GVVMLMYLRH AIEAVPSLNN 960
  961 PQTFSEQKLD EALYHGAVLR VRPKAMTVAV IIAGLLPILW GTGAGSEVMS RIAAPMIGGM1020
 1021 ITAPLLSLFI IPAAYKLMWL HRHRVRK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1000.0
Match:	1oy6A
Description:	Multidrug efflux transporter AcrB pore domain; PN1, PN2, PC1 and PC2 subdomains; Multidrug efflux transporter AcrB TolC docking domain; DN and DC subdomains; Multidrug efflux transporter AcrB transmembrane domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
cation transmembrane transporter activity	2.18377168861769	bayes_pls_golite062009
ion transmembrane transporter activity	2.13718030205691	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.90624016306311	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.42126232816819	bayes_pls_golite062009
transporter activity	1.3820077421337	bayes_pls_golite062009
transmembrane transporter activity	1.24821957412828	bayes_pls_golite062009
	0.91142062580843	bayes_pls_golite062009
drug transmembrane transporter activity	0.84711276738506	bayes_pls_golite062009
	0.76343319945017	bayes_pls_golite062009
substrate-specific transporter activity	0.12397386858091	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: