YRC Public Data Repository - Protein Structure Prediction

Protein:	BUD14
Organism:	Saccharomyces cerevisiae
Length:	707 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BUD14.

Description	E-value	Query Range	Subject Range
BUD14 - Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhi...	8.0E-90	[1..707]	[1..707]
MDN1_GIAIN - Midasin OS=Giardia intestinalis GN=MDN1 PE=3 SV=1	7.0E-60	[61..613]	[4049..4548]
GL50803_39312 - \| organism=Giardia_Assemblage_A_isolate_WB \| product=Midasin \| location=GLCHR02:40567-55074(+) \| len... ctg02_53\|39312\|M... - null gi\|157432603, gi... - gi\|71070869\|ref\|XP_768005.1\| hypothetical protein GLP_74_7403_21910 [Giardia lamblia ATCC 50803], gi...	7.0E-60	[61..613]	[4049..4548]
gi\|42549477, gi\|... - gi\|46116552\|ref\|XP_384294.1\| hypothetical protein FG04118.1 [Gibberella zeae PH-1], gi\|42549477\|gb\|E...	2.0E-50	[112..663]	[210..803]
gi\|49086366, gi\|... - gi\|67517841\|ref\|XP_658703.1\| hypothetical protein AN1099.2 [Aspergillus nidulans FGSC A4], ref\|XP_40...	3.0E-45	[149..633]	[10..486]
gi\|32470954, gi\|... - gi\|32470954\|ref\|NP_863947.1\| myosin heavy chain [Rhodopirellula baltica SH 1], gi\|32443099\|emb\|CAD71...	2.0E-44	[331..668]	[538..878]
gi\|44983865 - gi\|44983865\|gb\|AAS52901.1\| AER220Cp [Ashbya gossypii ATCC 10895] gi\|44983865, gi\|... - gi\|45190823\|ref\|NP_985077.1\| AER220Cp [Ashbya gossypii ATCC 10895], gi\|44983865\|gb\|AAS52901.1\| AER22...	2.0E-39	[39..585]	[16..568]
CG13185-PB - This gene is referred to in FlyBase by the symbol Dmel\CG13185 (CG13185, FBgn0033661). It is a prote...	2.0E-39	[2..484]	[4462..4956]
gi\|85082837 - gi\|85082837\|ref\|XP_956993.1\| hypothetical protein [Neurospora crassa OR74A] gi\|32402958, gi\|... - gi\|32402958\|ref\|XP_322092.1\| hypothetical protein [Neurospora crassa], gi\|28918076\|gb\|EAA27757.1\| hy...	1.0E-38	[211..632]	[290..721]

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Predicted Domain #1

Region A:
Residues: [1-186]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL NDPTLIEDYS  60
   61 DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN SKLNILSNAA EKGHDVLRNR 120
  121 DDDKELEEEN VEKHMHSNSK RDQRHYKENS SELPDSYDYS DSEFEDNLER RLQEIETDSV 180
  181 DSADKD

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [187-259]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EVHFSVNNTM NPDVDDFSDG LKYAISEDED EEENYSDDDD FDRKFQDSGF QGEKDDLEEE  60
   61 NDDYQPLSPP REL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
binding	2.48735671726158	bayes_pls_golite062009
protein binding	2.14586566310919	bayes_pls_golite062009
cytoskeletal protein binding	2.02807082768447	bayes_pls_golite062009
actin binding	1.61769239689139	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.29663830666504	bayes_pls_golite062009
transcription regulator activity	1.28186497060277	bayes_pls_golite062009
nucleic acid binding	1.22075469862949	bayes_pls_golite062009
kinase activity	1.07563262976041	bayes_pls_golite062009
DNA binding	0.96398527572881	bayes_pls_golite062009
ligase activity	0.701001687425066	bayes_pls_golite062009
actin filament binding	0.58289829357591	bayes_pls_golite062009
structural molecule activity	0.537901980414372	bayes_pls_golite062009
molecular adaptor activity	0.494024302340603	bayes_pls_golite062009
SH3/SH2 adaptor activity	0.409062479526042	bayes_pls_golite062009
protein binding, bridging	0.370788151516215	bayes_pls_golite062009
transcription factor activity	0.256358801491972	bayes_pls_golite062009
signal transducer activity	0.150891693412241	bayes_pls_golite062009
molecular transducer activity	0.150891693412241	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [260-419]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DPDKLYALYA FNGHDSSHCQ LGQDEPCILL NDQDAYWWLV KRITDGKIGF APAEILETFP  60
   61 ERLARLNCWK NENMSSQSVA SSDSKDDSIS SGNKNQSDAE SIIPTPALNG YGKGNKSVSF 120
  121 NDVVGYADRF IDDAIEDTSL DSNDDGGEGN GQSYDDDVDN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	33.0
Match:	1g83A_
Description:	Fyn proto-oncogene tyrosine kinase, SH3 domain; Tyrosine kinase Fyn
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [420-491]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DKETKVTHRD EYTEAKLNFA KFQDDDTSDV VSDVSFSTSL NTPLNVKKVR RQDNKNESEP  60
   61 KTSSSKDRED DY

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #5

Region A:
Residues: [492-600]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NANRYVGQEK SEPVDSDYDT DLKKVFEAPR MPFANGMAKS DSQNSLSTIG EFSPSSSEWT  60
   61 NESPSTPIVE ESSSIPSSRA IRTFTYIMQN RKLRDNKRGK HRGQIQASL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #6

Region A:
Residues: [601-707]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GSSGGMPNQT DAEQPKEELE KHHSTPEEEK QSTLSLHSSS EEDFYMDEQR AVSSASINSS  60
   61 LSGSRALSNT NMSDPASKPN SLVQHLYAPV FDRMDVLMKQ LDEIIRK

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: