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View Structure Prediction Details

Protein: PFK27
Organism: Saccharomyces cerevisiae
Length: 397 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PFK27.

Description E-value Query
Range
Subject
Range
6P22_YEAST - 6-phosphofructo-2-kinase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PFK27 PE=1 SV...
PFK27 - 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenol...
gi|207341472 - gi|207341472|gb|EDZ69520.1| YOL136Cp-like protein [Saccharomyces cerevisiae AWRI1631]
gi|190407217 - gi|190407217|gb|EDV10484.1| 6-phosphofructo-2-kinase [Saccharomyces cerevisiae RM11-1a]
0.0 [1..397] [1..397]
gi|162463489, gi... - gi|162463489|ref|NP_001105044.1| fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase [Zea mays...
6.0E-88 [4..326] [253..548]
gi|14149116 - gi|14149116|dbj|BAB55655.1| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase [Bruguiera gym...
1.0E-87 [7..326] [256..539]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
2.0E-86 [11..326] [17..296]
F261_RAT - 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 OS=Rattus norvegicus GN=Pfkfb1 PE=1 SV=3
4.0E-86 [59..326] [1..239]
PFKFB1 - 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
1.0E-85 [59..326] [1..239]
F26_LITCT - 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase OS=Lithobates catesbeiana PE=2 SV=1
1.0E-85 [65..326] [6..238]
gi|18251459 - gi|18251459|gb|AAL66023.1| fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase [Oryza sativa]
1.0E-85 [13..326] [260..548]

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Predicted Domain #1
Region A:
Residues: [1-327]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGGSSDSDSH DGYLTSEYNS SNSLFSLNTG NSYSSASLDR ATLDCQDSVF FDNHKSSLLS  60
   61 TEVPRFISND PLHLPITLNY KRDNADPTYT NGKVNKFMIV LIGLPATGKS TISSHLIQCL 120
  121 KNNPLTNSLR CKVFNAGKIR RQISCATISK PLLLSNTSSE DLFNPKNNDK KETYARITLQ 180
  181 KLFHEINNDE CDVGIFDATN STIERRRFIF EEVCSFNTDE LSSFNLVPII LQVSCFNRSF 240
  241 IKYNIHNKSF NEDYLDKPYE LAIKDFAKRL KHYYSQFTPF SLDEFNQIHR YISQHEEIDT 300
  301 SLFFFNVINA GVVEPHSLNQ SHYPSTC

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 152.211549
Match: 2bifA_
Description: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, kinase domain; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphatase domain
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [328-397]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GKQIRDTIMV IENFINHYSQ MFGFEYIEAV KLFFESFGNS SEETLTTLDS VVNDKFFDDL  60
   61 QSLIESNGFA 

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.849 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.881 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.833 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.829 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.791 a.4.1 Homeodomain-like
View Download 0.782 a.144.1 PABC (PABP) domain
View Download 0.728 a.150.1 Anti-sigma factor AsiA
View Download 0.715 a.60.1 SAM/Pointed domain
View Download 0.711 a.60.13 Putative methyltransferase TM0872, insert domain
View Download 0.663 a.4.5 "Winged helix" DNA-binding domain
View Download 0.629 a.4.5 "Winged helix" DNA-binding domain
View Download 0.625 a.8.1 Bacterial immunoglobulin/albumin-binding domains
View Download 0.622 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.605 a.60.12 DNA polymerase beta-like, second domain
View Download 0.575 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.575 a.4.5 "Winged helix" DNA-binding domain
View Download 0.573 a.4.1 Homeodomain-like
View Download 0.564 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.556 a.5.2 UBA-like
View Download 0.544 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.541 a.144.1 PABC (PABP) domain
View Download 0.535 a.64.1 Saposin
View Download 0.517 a.13.1 alpha-2-Macroglobulin receptor associated protein (RAP) domain 1
View Download 0.507 a.8.1 Bacterial immunoglobulin/albumin-binding domains
View Download 0.498 a.150.1 Anti-sigma factor AsiA
View Download 0.493 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.492 a.159.2 FF domain
View Download 0.488 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.483 a.5.1 DNA helicase RuvA subunit, C-terminal domain
View Download 0.479 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.478 a.4.1 Homeodomain-like
View Download 0.476 f.13.1 Family A G protein-coupled receptor-like
View Download 0.475 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.466 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.457 a.4.9 Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View Download 0.447 a.65.1 Annexin
View Download 0.441 a.4.6 C-terminal effector domain of the bipartite response regulators
View Download 0.435 a.7.7 BAG domain
View Download 0.429 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.419 a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.415 a.150.1 Anti-sigma factor AsiA
View Download 0.407 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.401 a.17.1 p8-MTCP1
View Download 0.395 d.48.1 RecA protein, C-terminal domain
View Download 0.392 a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.392 a.17.1 p8-MTCP1
View Download 0.392 a.2.1 GreA transcript cleavage protein, N-terminal domain
View Download 0.390 a.60.13 Putative methyltransferase TM0872, insert domain
View Download 0.388 d.8.1 Urease, gamma-subunit
View Download 0.385 a.4.9 Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View Download 0.381 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.376 a.5.6 Hypothetical protein MTH1615
View Download 0.374 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.374 d.54.1 Enolase N-terminal domain-like
View Download 0.371 a.2.7 tRNA-binding arm
View Download 0.358 a.4.9 Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View Download 0.357 d.188.1 Prokaryotic ribosomal protein L17
View Download 0.356 a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.355 d.50.1 dsRNA-binding domain-like
View Download 0.352 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.351 a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.350 a.17.1 p8-MTCP1
View Download 0.348 a.60.1 SAM/Pointed domain
View Download 0.348 a.39.1 EF-hand
View Download 0.347 a.52.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View Download 0.343 a.60.1 SAM/Pointed domain
View Download 0.341 d.51.1 Eukaryotic type KH-domain (KH-domain type I)
View Download 0.339 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.338 a.60.13 Putative methyltransferase TM0872, insert domain
View Download 0.338 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.337 a.144.1 PABC (PABP) domain
View Download 0.335 a.5.2 UBA-like
View Download 0.334 a.4.11 RNA polymerase subunit RPB10
View Download 0.331 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.330 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.323 a.64.2 Bacteriocin AS-48
View Download 0.322 a.60.12 DNA polymerase beta-like, second domain
View Download 0.319 a.163.1 Crustacean CHH/MIH/GIH neurohormone
View Download 0.318 a.7.7 BAG domain
View Download 0.318 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.316 i.1.1 Ribosome and ribosomal fragments
View Download 0.316 a.4.6 C-terminal effector domain of the bipartite response regulators
View Download 0.314 a.5.2 UBA-like
View Download 0.313 a.76.1 Iron-dependent represor protein, dimerization domain
View Download 0.312 a.60.1 SAM/Pointed domain
View Download 0.311 a.30.2 Homodimeric domain of signal transducing histidine kinase
View Download 0.310 a.60.11 Hypothetical protein YjbJ
View Download 0.306 a.64.1 Saposin
View Download 0.304 a.118.9 ENTH/VHS domain
View Download 0.303 a.164.1 C-terminal domain of DFF45/ICAD (DFF-C domain)
View Download 0.302 a.74.1 Cyclin-like
View Download 0.299 i.1.1 Ribosome and ribosomal fragments
View Download 0.299 a.4.5 "Winged helix" DNA-binding domain
View Download 0.298 a.60.1 SAM/Pointed domain
View Download 0.298 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.295 d.39.1 Dynein light chain 8 (DLC8)
View Download 0.294 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.291 a.4.1 Homeodomain-like
View Download 0.288 a.12.1 Kix domain of CBP (creb binding protein)
View Download 0.286 a.58.1 Chemotaxis receptor methyltransferase CheR, N-terminal domain
View Download 0.285 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.283 a.60.12 DNA polymerase beta-like, second domain
View Download 0.281 d.15.1 Ubiquitin-like
View Download 0.278 a.30.2 Homodimeric domain of signal transducing histidine kinase
View Download 0.277 a.4.5 "Winged helix" DNA-binding domain
View Download 0.277 a.61.1 Retroviral matrix proteins
View Download 0.275 a.60.9 lambda integrase-like, N-terminal domain
View Download 0.274 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.274 a.23.4 Mitochondrial import receptor subunit Tom20
View Download 0.273 a.60.12 DNA polymerase beta-like, second domain
View Download 0.272 a.21.1 HMG-box
View Download 0.272 d.43.1 Elongation factor Ts (EF-Ts), dimerisation domain
View Download 0.271 a.5.7 4-hydroxy-2-oxovalerate aldolase DmpG, communication domain
View Download 0.266 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.265 a.4.5 "Winged helix" DNA-binding domain
View Download 0.265 a.135.1 Tetraspanin
View Download 0.265 a.3.1 Cytochrome c
View Download 0.265 a.69.3 1-deoxy-D-xylulose-5-phosphate reductoisomerase, C-terminal domain
View Download 0.262 a.5.1 DNA helicase RuvA subunit, C-terminal domain
View Download 0.260 a.76.1 Iron-dependent represor protein, dimerization domain
View Download 0.259 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.258 d.58.7 RNA-binding domain, RBD
View Download 0.257 a.4.5 "Winged helix" DNA-binding domain
View Download 0.256 a.48.3 Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View Download 0.255 d.58.44 Multidrug efflux transporter AcrB pore domain; PN1, PN2, PC1 and PC2 subdomains
View Download 0.252 d.52.5 Probable GTPase Der, C-terminal domain
View Download 0.251 a.118.1 ARM repeat
View Download 0.249 a.118.8 TPR-like
View Download 0.249 d.203.1 DsrC, the gamma subunit of dissimilatory sulfite reductase
View Download 0.247 a.179.1 Replisome organizer (g39p helicase loader/inhibitor protein)
View Download 0.246 a.2.1 GreA transcript cleavage protein, N-terminal domain
View Download 0.244 a.30.1 ROP protein
View Download 0.243 a.60.6 DNA polymerase beta, N-terminal domain-like
View Download 0.243 d.45.1 ClpS-like
View Download 0.243 a.23.4 Mitochondrial import receptor subunit Tom20
View Download 0.242 a.22.1 Histone-fold
View Download 0.242 a.105.1 FIS-like
View Download 0.241 a.28.3 Retrovirus capsid protein C-terminal domain
View Download 0.240 a.39.1 EF-hand
View Download 0.239 d.58.25 Killer toxin KP6 alpha-subunit
View Download 0.239 a.48.3 Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View Download 0.238 a.4.6 C-terminal effector domain of the bipartite response regulators
View Download 0.238 a.77.1 DEATH domain
View Download 0.238 a.58.1 Chemotaxis receptor methyltransferase CheR, N-terminal domain
View Download 0.236 a.8.2 Plasmid maintenance system epsilon/zeta, antidote epsilon subunit
View Download 0.234 d.94.1 HPr-like
View Download 0.234 a.7.6 Ribosomal protein S20
View Download 0.233 a.5.1 DNA helicase RuvA subunit, C-terminal domain
View Download 0.233 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.232 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.231 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.231 a.2.10 Epsilon subunit of F1F0-ATP synthase C-terminal domain
View Download 0.230 a.39.1 EF-hand
View Download 0.229 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.229 a.165.1 Myosin phosphatase inhibitor 17kDa protein, CPI-17
View Download 0.229 a.1.1 Globin-like
View Download 0.227 a.28.3 Retrovirus capsid protein C-terminal domain
View Download 0.225 a.74.1 Cyclin-like
View Download 0.225 a.39.1 EF-hand
View Download 0.223 a.24.7 FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)
View Download 0.223 a.60.11 Hypothetical protein YjbJ
View Download 0.221 a.21.1 HMG-box
View Download 0.220 a.4.1 Homeodomain-like
View Download 0.215 a.60.5 Barrier-to-autointegration factor, BAF
View Download 0.215 a.144.1 PABC (PABP) domain
View Download 0.214 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.214 a.4.5 "Winged helix" DNA-binding domain
View Download 0.213 d.106.1 Sterol carrier protein, SCP
View Download 0.213 a.16.1 S15/NS1 RNA-binding domain
View Download 0.211 a.60.2 RuvA domain 2-like
View Download 0.210 a.77.1 DEATH domain
View Download 0.210 a.11.1 Acyl-CoA binding protein
View Download 0.207 a.4.5 "Winged helix" DNA-binding domain
View Download 0.207 a.4.1 Homeodomain-like
View Download 0.206 a.71.1 Endoplasmic reticulum protein ERP29, C-domain
View Download 0.206 a.17.1 p8-MTCP1
View Download 0.206 a.60.13 Putative methyltransferase TM0872, insert domain
View Download 0.205 d.52.5 Probable GTPase Der, C-terminal domain
View Download 0.204 a.4.13 Sigma3 and sigma4 domains of RNA polymerase sigma factors
View Download 0.204 a.4.1 Homeodomain-like
View Download 0.200 a.74.1 Cyclin-like


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle