YRC Public Data Repository - Protein Structure Prediction

Protein:	KAR5
Organism:	Saccharomyces cerevisiae
Length:	504 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for KAR5.

Description	E-value	Query Range	Subject Range
KAR5 - Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a solu... KAR5_YEAST - Nuclear fusion protein KAR5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=KAR5 PE=1 SV...	0.0	[1..504]	[1..504]
gi\|2506923, gi\|1... - gi\|2506923\|sp\|Q09684.2\|KAR5_SCHPO RecName: Full=Nuclear fusion protein tht1; AltName: Full=Twin hors... KAR5_SCHPO - Nuclear fusion protein tht1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tht1 PE=1 SV=2	3.0E-98	[6..277]	[12..277]
gi\|19113843 - gi\|19113843\|ref\|NP_592931.1\| hypothetical protein SPAC13C5.03 [Schizosaccharomyces pombe 972h-] tht1 - nuclear membrane protein involved in karyogamy	3.0E-93	[31..277]	[4..243]

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Predicted Domain #1

Region A:
Residues: [1-108]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFEMRYVYLF AICIKFVSSS ELGKINNLLQ GRLIYTDNSV ATNVLESKFP FLKSTCVKDA  60
   61 LKLFLPQCIA NGLESIDAET RVETAIKLSI CEFQASGLGE IPENCMVD

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [109-205]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DLGSMMDCMF ELESSSQWWT TYSGNYQRLS SICYENLLPF EKEQILKLFL NITELYDSFG  60
   61 DDVDTKLNHL MFQMEQDSQN FLDDLARMFR NYDNELR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [248-344]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LMNEVDTVHH IMSDLADELA KNDIKSKIND LKDDSLNNLQ DLVEMSNDVK EYYSRNNKLV  60
   61 NTELENFSMG LKKQLGGMSK DLSESQMEAI ELLQGFN

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [408-504]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKLDDIEIRV SLVHKNVMTM MRVLDFMWKT SKMIIRCGYL AVKNKYYWLL CSVVWIWSKY  60
   61 RTSRVNVKMI PIKRYYQWAA LLLSIYLGAK TGSLIDF

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	7.42
Match:	3btaA
Description:	Botulinum neurotoxin
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [206-247]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NATESNRIIL ENDLSFFRNK VNDVLYETSE QLEVQIIEKN SQ

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [345-407]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SILHDSLLPS MTDEIVPEMT NFKNTLLQEW TAITSTLNGD FALWNEEIFS TFNDISEKLN  60
   61 GTK

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	7.42
Match:	3btaA
Description:	Botulinum neurotoxin
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits