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View Structure Prediction Details

Protein: VBA5
Organism: Saccharomyces cerevisiae
Length: 582 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for VBA5.

Description E-value Query
Range
Subject
Range
VBA5_YEAST - Vacuolar basic amino acid transporter 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=...
VBA5 - Putative transporter of the Major Facilitator Superfamily (MFS); proposed role as a basic amino acid...
3.0E-88 [1..582] [1..582]
gi|21225257, gi|... - gi|7649556|emb|CAB89031.1| putative membrane transport protein. [Streptomyces coelicolor A3(2)], gi|...
2.0E-80 [1..572] [1..525]
gi|16944692 - gi|16944692|emb|CAC28808.2| related to tetracycline efflux protein (otrb) [Neurospora crassa]
7.0E-75 [3..582] [2..575]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
7.0E-74 [3..566] [13..530]
gi|15528453 - gi|15528453|emb|CAC69145.1| putative membrane protein [Pichia anomala]
1.0E-72 [2..578] [65..608]
gi|19386936 - gi|19386936|gb|AAL87047.1|AF448056_3 putative dothistromin transporter [Mycosphaerella pini]
gi|19386936 - gi|19386936|gb|AAL87047.1|AF448056_3 putative dothistromin transporter [Mycosphaerella pini]
1.0E-72 [2..574] [25..574]
gi|25498023 - pir||D96902 MDR-type permease [imported] - Clostridium acetobutylicum
gi|15893318, gi|... - gi|15893318|ref|NP_346667.1| MDR-type permease [Clostridium acetobutylicum ATCC 824], gi|15022839|gb...
2.0E-72 [34..499] [4..418]
gi|14389419 - gi|14389419|gb|AAF64435.2|AF238225_1 DHA14-like major facilitator [Botryotinia fuckeliana]
3.0E-71 [2..572] [68..594]

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Predicted Domain #1
Region A:
Residues: [1-242]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEETKYSSQQ EIEGACGSDA SLNARGSNDS PMGLSLYLCL ASLTLVLFIT ALDILIVGTI  60
   61 IDVVAEQFGN YSKTGWLVTG YSLPNAILSL IWGRFASIIG FQHSLILAIL IFEAGSLIAA 120
  121 LASSMNMLIF GRVVAGVGGS GLQTLCFVIG CTMVGERSRP LVISILSCAF AVAAIVGPII 180
  181 GGAFTTHVTW RWCFYINLPI GGLAIIMFLL TYKAENKGIL QQIKDAIGTI SSFTFSKFRH 240
  241 QV

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 9.154902
Match: 1lviA_
Description: No description for 1lviA_ was found.

Predicted functions:

Term Confidence Notes
transporter activity 4.09176719149278 bayes_pls_golite062009
substrate-specific transporter activity 3.59744164024479 bayes_pls_golite062009
transmembrane transporter activity 3.5865018981083 bayes_pls_golite062009
substrate-specific transmembrane transporter activity 3.54194478414824 bayes_pls_golite062009
ion transmembrane transporter activity 2.21266150105753 bayes_pls_golite062009
organic acid transmembrane transporter activity 2.15783210522667 bayes_pls_golite062009
cation transmembrane transporter activity 1.79369588808394 bayes_pls_golite062009
active transmembrane transporter activity 1.59419818573076 bayes_pls_golite062009
secondary active transmembrane transporter activity 1.22264637452409 bayes_pls_golite062009
binding 1.01584233411372 bayes_pls_golite062009
polyol transmembrane transporter activity 0.924836669821548 bayes_pls_golite062009
alcohol transmembrane transporter activity 0.924836669821548 bayes_pls_golite062009
glycerol transmembrane transporter activity 0.812001619402678 bayes_pls_golite062009
symporter activity 0.74591237387473 bayes_pls_golite062009
solute:cation symporter activity 0.74591237387473 bayes_pls_golite062009
cation:sugar symporter activity 0.143419068761182 bayes_pls_golite062009
sugar:hydrogen symporter activity 0.143419068761182 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [243-314]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NFKRLMNGII FKFDFFGFAL CSAGLVLFLL GLTFGGNKYS WNSGQVITYL VLGVLLFIFS  60
   61 LVYDFFLFDK FN

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.790 a.2.1 GreA transcript cleavage protein, N-terminal domain
View Download 0.601 a.2.7 tRNA-binding arm
View Download 0.585 a.144.2 Ribosomal protein L20
View Download 0.561 a.76.1 Iron-dependent represor protein, dimerization domain
View Download 0.538 a.4.5 "Winged helix" DNA-binding domain
View Download 0.536 a.60.9 lambda integrase-like, N-terminal domain
View Download 0.519 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.511 a.2.7 tRNA-binding arm
View Download 0.498 a.77.1 DEATH domain
View Download 0.482 a.60.11 Hypothetical protein YjbJ
View Download 0.474 a.30.2 Homodimeric domain of signal transducing histidine kinase
View Download 0.461 d.58.17 Metal-binding domain
View Download 0.447 a.60.3 C-terminal domain of RNA polymerase alpha subunit
View Download 0.444 f.28.1 Nonheme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
View Download 0.432 a.4.5 "Winged helix" DNA-binding domain
View Download 0.431 d.57.1 DNA damage-inducible protein DinI
View Download 0.430 a.6.1 Putative DNA-binding domain
View Download 0.427 f.14.1 Voltage-gated potassium channels
View Download 0.422 a.59.1 PAH2 domain
View Download 0.402 d.58.17 Metal-binding domain
View Download 0.401 a.7.2 Enzyme IIa from lactose specific PTS, IIa-lac
View Download 0.393 a.76.1 Iron-dependent represor protein, dimerization domain
View Download 0.392 a.144.1 PABC (PABP) domain
View Download 0.389 a.2.8 Eukaryotic DNA topoisomerase I, dispensable insert domain
View Download 0.388 a.8.1 Bacterial immunoglobulin/albumin-binding domains
View Download 0.388 a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.380 a.4.8 Ribosomal protein S18
View Download 0.379 a.27.1 Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
View Download 0.364 a.5.7 4-hydroxy-2-oxovalerate aldolase DmpG, communication domain
View Download 0.363 a.4.5 "Winged helix" DNA-binding domain
View Download 0.363 d.58.10 Acylphosphatase-like
View Download 0.360 i.1.1 Ribosome and ribosomal fragments
View Download 0.359 a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.347 a.77.1 DEATH domain
View Download 0.344 a.46.2 Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain
View Download 0.342 d.43.1 Elongation factor Ts (EF-Ts), dimerisation domain
View Download 0.340 d.50.3 PI-Pfui intein middle domain
View Download 0.333 a.24.7 FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)
View Download 0.333 d.58.49 YajQ-like
View Download 0.329 a.2.10 Epsilon subunit of F1F0-ATP synthase C-terminal domain
View Download 0.326 d.58.40 D-ribose-5-phosphate isomerase (RpiA), lid domain
View Download 0.325 a.4.5 "Winged helix" DNA-binding domain
View Download 0.319 d.58.11 EF-G/eEF-2 domains III and V
View Download 0.317 d.58.7 RNA-binding domain, RBD
View Download 0.317 a.112.1 Description not found.
View Download 0.314 d.50.1 dsRNA-binding domain-like
View Download 0.312 a.44.1 Disulphide-bond formation facilitator (DSBA), insertion domain
View Download 0.309 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.306 a.2.2 Ribosomal protein L29 (L29p)
View Download 0.306 a.58.1 Chemotaxis receptor methyltransferase CheR, N-terminal domain
View Download 0.306 a.4.9 Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View Download 0.301 a.144.1 PABC (PABP) domain
View Download 0.299 a.74.1 Cyclin-like
View Download 0.294 d.48.1 RecA protein, C-terminal domain
View Download 0.293 a.16.1 S15/NS1 RNA-binding domain
View Download 0.286 a.156.1 S13-like H2TH domain
View Download 0.286 a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.285 a.47.2 t-snare proteins
View Download 0.282 a.23.1 HSC20 (HSCB), C-terminal oligomerisation domain
View Download 0.281 a.64.1 Saposin
View Download 0.279 d.58.7 RNA-binding domain, RBD
View Download 0.245 a.77.1 DEATH domain
View Download 0.242 a.13.1 alpha-2-Macroglobulin receptor associated protein (RAP) domain 1
View Download 0.242 a.74.1 Cyclin-like
View Download 0.239 a.32.1 Transcription factor IIA (TFIIA), alpha-helical domain
View Download 0.236 a.8.1 Bacterial immunoglobulin/albumin-binding domains
View Download 0.232 a.35.1 lambda repressor-like DNA-binding domains
View Download 0.231 a.38.1 Helix-loop-helix DNA-binding domain
View Download 0.231 a.177.1 Sigma2 domain of RNA polymerase sigma factors
View Download 0.226 d.58.1 4Fe-4S ferredoxins
View Download 0.226 a.108.1 Ribosomal protein L7/12, oligomerisation (N-terminal) domain
View Download 0.223 a.5.2 UBA-like
View Download 0.218 a.2.11 Fe,Mn superoxide dismutase (SOD), N-terminal domain
View Download 0.212 d.58.43 Mechanosensitive channel protein MscS (YggB), C-terminal domain
View Download 0.212 a.3.1 Cytochrome c
View Download 0.208 a.7.5 Tubulin chaperone cofactor A

Predicted Domain #3
Region A:
Residues: [315-526]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PEPDNISYRP LLLRRLVAKP AIIIVNMVTF LLCTGYNGQM IYSVQFFQLI FASSAWKAGL  60
   61 HLIPIVITNV IAAIASGVIT KKLGLVKPLL IFGGVLGVIG AGLMTLMTNT STKSTQIGVL 120
  121 LLPGFSLGFA LQASLMSAQL QITKDRPEAA MDFIEVTAFN TFMKSLGTTL GGVLSTTVFS 180
  181 ASFHNKVSRA HLEPYEGKTV DDMILYRLQN YD

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [527-582]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GSHSTIGNIL SDSIKNVFWM DLGFYALGFL FCSFSSNKKL IIPKKDDTPE DNLEDK

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.655 d.47.1 Ribosomal protein L11, N-terminal domain
View Download 0.305 a.5.2 UBA-like
View Download 0.299 a.140.2 SAP domain
View Download 0.289 d.47.1 Ribosomal protein L11, N-terminal domain
View Download 0.238 a.65.1 Annexin
View Download 0.201 g.34.1 HIV-1 VPU cytoplasmic domain


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle