YRC Public Data Repository - Protein Structure Prediction

Protein:	BCK1
Organism:	Saccharomyces cerevisiae
Length:	1478 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BCK1.

Description	E-value	Query Range	Subject Range
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[1..1475]	[1..1338]
gi\|218490 - gi\|218490\|dbj\|BAA01226.1\| Ssp31 protein kinase [Saccharomyces cerevisiae]	0.0	[1..1478]	[1..1478]
gi\|189491751, gi... - gi\|57284082\|emb\|CAI43167.1\| p21 protein (Cdc42/Rac)-activated kinase 3 [Homo sapiens], gi\|57284006\|e... gi\|157170250 - gi\|157170250\|gb\|AAI52762.1\| P21 protein (Cdc42/Rac)-activated kinase 3 [synthetic construct] ENSPTRT00000049237 - null	0.0	[895..1462]	[4..541]
gi\|3420949 - gi\|3420949\|gb\|AAC31969.1\| Cdc42/Rac effector kinase; PAK-3 [Mus musculus]	0.0	[895..1462]	[4..541]
PAK3_RAT - Serine/threonine-protein kinase PAK 3 OS=Rattus norvegicus GN=Pak3 PE=1 SV=1	0.0	[895..1462]	[4..541]
gi\|1168176 - gi\|1168176\|gb\|AAB35358.1\| mPAK-3=p21Cdc42/Rac activated kinase [mice, fibroblast, Peptide, 543 aa]	0.0	[895..1462]	[4..541]

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Predicted Domain #1

Region A:
Residues: [1-653]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPFLRKIAGT AHTHSRSDSN SSVKFGHQPT SSVASTKSSS KSPRATSRKS IYDDIRSQFP  60
   61 NLTPNSTSSQ FYESTPVIEQ SFNWTTDDHI SAGTLENPTS FTNSSYKNDN GPSSLSDSRK 120
  121 SSGGNSVNSL SFDKLILSWD PTDPDEWTMH RVTSWFKFHD FPESWILFFK KHQLFGHRFI 180
  181 KLLAYDNFAV YEKYLPQTKT ASYTRFQQLL KKTMTKNVTN SHIRQKSASK LKSSRSSSES 240
  241 IKSKLKNSKS QEDISNSRST SESALSPTKS GPSKTDEKNF LHSTSTHQKT KSASSLYRRS 300
  301 FISLRGSSSS NASSAKSPSN IKLSIPARPH SIIESNSTLT KSASPPASPS YPSIFRRHHK 360
  361 SSSSESSLLN SLFGSGIGEE APTKPNPQGH SLSSENLAKG KSKHYETNVS SPLKQSSLPT 420
  421 SDDKGNLWNK FKRKSQIGVP SPNTVAYVTS QETPSLKSNS STATLTVQTA DVNIPSPSSS 480
  481 PPPIPKTANR SLEVISTEDT PKISSTTASF KETYPDCINP DKTVPVPVNN QKYSVKNFLL 540
  541 DQKFYPLKKT GLNDSENKYI LVTKDNVSFV PLNLKSVAKL SSFKESALTK LGINHKNVTF 600
  601 HMTDFDCDIG AAIPDDTLEF LKKSLFLNTS GKIYIKDQMK LQQKPKPAPL TSE

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [654-949]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NNVPLKSVKS KSSMRSGTSS LIASTDDVSI VTSSSDITSF DEHASGSGRR YPQTPSYYYD  60
   61 RVSNTNPTEE LNYWNIKEVL SHEENAPKMV FKTSPKLELN LPDKGSKLNI PTPITENESK 120
  121 SSFQVLRKDE GTEIDFNHRR ESPYTKPELA PKREAPKPPA NTSPQRTLST SKQNKPIRLV 180
  181 RASTKISRSK RSKPLPPQLL SSPIEASSSS PDSLTSSYTP ASTHVLIPQP YKGANDVMRR 240
  241 LKTDQDSTST SPSLKMKQKV NRSNSTVSTS NSIFYSPSPL LKRGNSKRVV SSTSAA

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	10.04
Match:	1ldvA
Description:	No description for 1ldvA was found.

Predicted Domain #3

Region A:
Residues: [950-1032]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DIFEENDITF ADAPPMFDSD DSDDDSSSSD DIIWSKKKTA PETNNENKKD EKSDNSSTHS  60
   61 DEIFYDSQTQ DKMERKMTFR PSP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	15.0
Match:	1f3mA_
Description:	pak1 autoregulatory domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [1033-1129]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EVVYQNLEKF FPRANLDKPI TEGIASPTSP KSLDSLLSPK NVASSRTEPS TPSRPVPPDS  60
   61 SYEFIQDGLN GKNKPLNQAK TPKRTKTIRT IAHEASL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.351	a.3.1	Cytochrome c
View	Download	0.303	a.40.1	Calponin-homology domain, CH-domain
View	Download	0.207	a.40.1	Calponin-homology domain, CH-domain

Predicted Domain #5

Region A:
Residues: [1130-1259]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ARKNSVKLKR QNTKMWGTRM VEVTENHMVS INKAKNSKGE YKEFAWMKGE MIGKGSFGAV  60
   61 YLCLNVTTGE MMAVKQVEVP KYSSQNEAIL STVEALRSEV STLKDLDHLN IVQYLGFENK 120
  121 NNIYSLFLEY

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	486.9897
Match:	1f3mC_
Description:	pak1
Matching Structure (courtesy of the PDB):

Predicted Domain #6

Region A:
Residues: [1260-1478]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VAGGSVGSLI RMYGRFDEPL IKHLTTQVLK GLAYLHSKGI LHRDMKADNL LLDQDGICKI  60
   61 SDFGISRKSK DIYSNSDMTM RGTVFWMAPE MVDTKQGYSA KVDIWSLGCI VLEMFAGKRP 120
  121 WSNLEVVAAM FKIGKSKSAP PIPEDTLPLI SQIGRNFLDA CFEINPEKRP TANELLSHPF 180
  181 SEVNETFNFK STRLAKFIKS NDKLNSSKLR ITSQENKTE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	486.9897
Match:	1f3mC_
Description:	pak1
Matching Structure (courtesy of the PDB):

Predicted Domain #7

Region A:
Residues: [808-921]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 APKPPANTSP QRTLSTSKQN KPIRLVRAST KISRSKRSKP LPPQLLSSPI EASSSSPDSL  60
   61 TSSYTPASTH VLIPQPYKGA NDVMRRLKTD QDSTSTSPSL KMKQKVNRSN STVS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10.30103
Match:	2nptB
Description:	No description for 2nptB was found.

Predicted functions:

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	2.40869571527615	bayes_pls_golite062009
protein kinase activity	2.33375027480966	bayes_pls_golite062009
kinase activity	2.3070634049589	bayes_pls_golite062009
binding	2.24212855645397	bayes_pls_golite062009
protein binding	2.09596244214119	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	2.07643991042071	bayes_pls_golite062009
protein serine/threonine kinase activity	1.93849072962936	bayes_pls_golite062009
transferase activity	1.56694652741645	bayes_pls_golite062009
signal transducer activity	1.24594754122874	bayes_pls_golite062009
molecular transducer activity	1.24594754122874	bayes_pls_golite062009
potassium channel regulator activity	1.20477942519661	bayes_pls_golite062009
phospholipase binding	0.746850275604721	bayes_pls_golite062009
kinase binding	0.452988418922855	bayes_pls_golite062009
protein kinase binding	0.438176837693626	bayes_pls_golite062009
	0.31361705040754	bayes_pls_golite062009
hydrolase activity	0.297499761800095	bayes_pls_golite062009
channel regulator activity	0.295510833740311	bayes_pls_golite062009
catalytic activity	0.178906437366734	bayes_pls_golite062009

Predicted Domain #8

Region A:
Residues: [922-1087]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TSNSIFYSPS PLLKRGNSKR VVSSTSAADI FEENDITFAD APPMFDSDDS DDDSSSSDDI  60
   61 IWSKKKTAPE TNNENKKDEK SDNSSTHSDE IFYDSQTQDK MERKMTFRPS PEVVYQNLEK 120
  121 FFPRANLDKP ITEGIASPTS PKSLDSLLSP KNVASSRTEP STPSRP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	16.221849
Match:	1f3mA
Description:	pak1 autoregulatory domain
Matching Structure (courtesy of the PDB):

Predicted Domain #9

Region A:
Residues: [1088-1171]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VPPDSSYEFI QDGLNGKNKP LNQAKTPKRT KTIRTIAHEA SLARKNSVKL KRQNTKMWGT  60
   61 RMVEVTENHM VSINKAKNSK GEYK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	34.30103
Match:	2ozoA
Description:	No description for 2ozoA was found.

Predicted Domain #10

Region A:
Residues: [1172-1478]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EFAWMKGEMI GKGSFGAVYL CLNVTTGEMM AVKQVEVPKY SSQNEAILST VEALRSEVST  60
   61 LKDLDHLNIV QYLGFENKNN IYSLFLEYVA GGSVGSLIRM YGRFDEPLIK HLTTQVLKGL 120
  121 AYLHSKGILH RDMKADNLLL DQDGICKISD FGISRKSKDI YSNSDMTMRG TVFWMAPEMV 180
  181 DTKQGYSAKV DIWSLGCIVL EMFAGKRPWS NLEVVAAMFK IGKSKSAPPI PEDTLPLISQ 240
  241 IGRNFLDACF EINPEKRPTA NELLSHPFSE VNETFNFKST RLAKFIKSND KLNSSKLRIT 300
  301 SQENKTE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	117.0
Match:	1zmuA
Description:	Catalytic and ubiqutin-associated domains of MARK2/PAR-1: Wild type
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: