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View Structure Prediction Details

Protein: PRP8
Organism: Saccharomyces cerevisiae
Length: 2413 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PRP8.

Description E-value Query
Range
Subject
Range
SPP42_SCHPO - Pre-mRNA-splicing factor spp42 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=spp42 PE=1 ...
spp42 - U5 snRNP complex subunit Spp42
0.0 [55..2413] [5..2363]
PRP8_YEAST - Pre-mRNA-splicing factor 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP8 PE=1 SV=...
PRP8 - Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutation...
0.0 [1..2413] [1..2413]
gi|19068795, gi|... - gi|19074153|ref|NP_584759.1| similarity to PRE-mRNA SPLICING FACTOR PRP8 [Encephalitozoon cuniculi G...
gi|19074153 - ref|NP_584759.1| similarity to PRE-mRNA SPLICING FACTOR PRP8 [Encephalitozoon cuniculi]
0.0 [808..2407] [782..2172]
gi|2463577 - gi|2463577|dbj|BAA22563.1| PRP8 protein [Homo sapiens]
0.0 [65..2413] [3..2335]
gi|13812348, gi|... - pir||F90109 splicing factor Prp8 [imported] - Guillardia theta nucleomorph, gi|162606442|ref|XP_0017...
0.0 [494..2231] [315..1952]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
0.0 [96..2411] [24..2398]
gi|15220049 - gi|15220049|ref|NP_178124.1| SUS2 (ABNORMAL SUSPENSOR 2) [Arabidopsis thaliana]
0.0 [62..2413] [12..2382]
gi|14719309 - gi|14719309|gb|AAK73127.1|AF397148_1 pre-mRNA processing factor 8 [Paramecium tetraurelia]
0.0 [106..2413] [13..2317]
gi|4757577 - gi|4757577|gb|AAD29088.1|AF115849_1 pre-mRNA processing 8 protein homolog PRP8 [Trichomonas vaginali...
0.0 [108..2412] [16..2319]
PRP8_MOUSE - Pre-mRNA-processing-splicing factor 8 OS=Mus musculus GN=Prpf8 PE=1 SV=1
0.0 [65..2413] [3..2335]

Back

Predicted Domain #1
Region A:
Residues: [1-237]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE  60
   61 INAEEIVDFT LPPPPPPPGL DELETKAEKK VELHGKRKLD IGKDTFVTRK SRKRAKKMTK 120
  121 KAKRSNLYTP KAEMPPEHLR KIINTHSDMA SKMYNTDKKA FLGALKYLPH AILKLLENMP 180
  181 HPWEQAKEVK VLYHTSGAIT FVNETPRVIE PVYTAQWSAT WIAMRREKRD RTHFKRM

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #2
Region A:
Residues: [238-803]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RFPPFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS  60
   61 YKKWSFDLPE MSNLYRLSTP LRDEVTDKNY YYLFDKKSFF NGKALNNAIP GGPKFEPLYP 120
  121 REEEEDYNEF NSIDRVIFRV PIRSEYKVAF PHLYNSRPRS VRIPWYNNPV SCIIQNDEEY 180
  181 DTPALFFDPS LNPIPHFIDN NSSLNVSNTK ENGDFTLPED FAPLLAEEEE LILPNTKDAM 240
  241 SLYHSPFPFN RTKGKMVRAQ DVALAKKWFL QHPDEEYPVK VKVSYQKLLK NYVLNELHPT 300
  301 LPTNHNKTKL LKSLKNTKYF QQTTIDWVEA GLQLCRQGHN MLNLLIHRKG LTYLHLDYNF 360
  361 NLKPTKTLTT KERKKSRLGN SFHLMRELLK MMKLIVDTHV QFRLGNVDAF QLADGIHYIL 420
  421 NHIGQLTGIY RYKYKVMHQI RACKDLKHII YYKFNKNLGK GPGCGFWQPA WRVWLNFLRG 480
  481 TIPLLERYIG NLITRQFEGR SNEIVKTTTK QRLDAYYDLE LRNSVMDDIL EMMPESIRQK 540
  541 KARTILQHLS EAWRCWKANI PWDVPG

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #3
Region A:
Residues: [804-1022]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPAPIKKIIE RYIKSKADAW VSAAHYNRER IKRGAHVEKT MVKKNLGRLT RLWIKNEQER  60
   61 QRQIQKNGPE ITPEEATTIF SVMVEWLESR SFSPIPFPPL TYKNDTKILV LALEDLKDVY 120
  121 ASKVRLNASE REELALIEEA YDNPHDTLNR IKKYLLTQRV FKPVDITMME NYQNISPVYS 180
  181 VDPLEKITDA YLDQYLWYEA DQRKLFPNWI KPSDSEIPP

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [1023-1282]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LLVYKWTQGI NNLSEIWDVS RGQSAVLLET TLGEMAEKID FTLLNRLLRL IVDPNIADYI  60
   61 TAKNNVVINF KDMSHVNKYG LIRGLKFASF IFQYYGLVID LLLLGQERAT DLAGPANNPN 120
  121 EFMQFKSKEV EKAHPIRLYT RYLDRIYMLF HFEEDEGEEL TDEYLAENPD PNFENSIGYN 180
  181 NRKCWPKDSR MRLIRQDVNL GRAVFWEIQS RVPTSLTSIK WENAFVSVYS KNNPNLLFSM 240
  241 CGFEVRILPR QRMEEVVSND 

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #5
Region A:
Residues: [1283-2068]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EGVWDLVDER TKQRTAKAYL KVSEEEIKKF DSRIRGILMA SGSTTFTKVA AKWNTSLISL  60
   61 FTYFREAIVA TEPLLDILVK GETRIQNRVK LGLNSKMPTR FPPAVFYTPK ELGGLGMISA 120
  121 SHILIPASDL SWSKQTDTGI THFRAGMTHE DEKLIPTIFR YITTWENEFL DSQRVWAEYA 180
  181 TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLERN 240
  241 SPFWWTNSHH DGKLWNLNAY RTDVIQALGG IETILEHTLF KGTGFNSWEG LFWEKASGFE 300
  301 DSMQFKKLTH AQRTGLSQIP NRRFTLWWSP TINRANVYVG FLVQLDLTGI FLHGKIPTLK 360
  361 ISLIQIFRAH LWQKIHESIV FDICQILDGE LDVLQIESVT KETVHPRKSY KMNSSAADIT 420
  421 MESVHEWEVS KPSLLHETND SFKGLITNKM WFDVQLRYGD YDSHDISRYV RAKFLDYTTD 480
  481 NVSMYPSPTG VMIGIDLAYN MYDAYGNWFN GLKPLIQNSM RTIMKANPAL YVLRERIRKG 540
  541 LQIYQSSVQE PFLNSSNYAE LFNNDIKLFV DDTNVYRVTV HKTFEGNVAT KAINGCIFTL 600
  601 NPKTGHLFLK IIHTSVWAGQ KRLSQLAKWK TAEEVSALVR SLPKEEQPKQ IIVTRKAMLD 660
  661 PLEVHMLDFP NIAIRPTELR LPFSAAMSID KLSDVVMKAT EPQMVLFNIY DDWLDRISSY 720
  721 TAFSRLTLLL RALKTNEESA KMILLSDPTI TIKSYHLWPS FTDEQWITIE SQMRDLILTE 780
  781 YGRKYN

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #6
Region A:
Residues: [2069-2151]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VNISALTQTE IKDIILGQNI KAPSVKRQKM AELEAARSEK QNDEEAAGAS TVMKTKTINA  60
   61 QGEEIVVVAS ADYESQTFSS KNE

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.627 0.886 pre-mRNA splicing factor activity c.47.1 Thioredoxin-like
View Download 0.481 0.886 pre-mRNA splicing factor activity d.122.1 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
View Download 0.582 0.886 pre-mRNA splicing factor activity d.88.1 SRF-like
View Download 0.694 0.886 pre-mRNA splicing factor activity d.52.1 Alpha-lytic protease prodomain
View Download 0.508 0.886 pre-mRNA splicing factor activity d.88.1 SRF-like
View Download 0.406 0.886 pre-mRNA splicing factor activity d.52.3 Prokaryotic type KH domain (KH-domain type II)
View Download 0.390 0.886 pre-mRNA splicing factor activity a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.380 0.886 pre-mRNA splicing factor activity a.64.1 Saposin
View Download 0.334 0.886 pre-mRNA splicing factor activity a.24.13 Domain of the SRP/SRP receptor G-proteins
View Download 0.323 0.886 pre-mRNA splicing factor activity a.22.1 Histone-fold
View Download 0.313 0.886 pre-mRNA splicing factor activity a.4.5 "Winged helix" DNA-binding domain
View Download 0.312 0.886 pre-mRNA splicing factor activity b.55.1 PH domain-like
View Download 0.303 0.886 pre-mRNA splicing factor activity b.55.1 PH domain-like
View Download 0.293 0.886 pre-mRNA splicing factor activity a.80.1 DNA polymerase III clamp loader subunits, C-terminal domain
View Download 0.291 0.886 pre-mRNA splicing factor activity d.8.1 Urease, gamma-subunit
View Download 0.281 0.886 pre-mRNA splicing factor activity b.61.3 D-aminopeptidase, middle and C-terminal domains
View Download 0.249 0.886 pre-mRNA splicing factor activity a.47.2 t-snare proteins
View Download 0.247 0.886 pre-mRNA splicing factor activity d.95.2 Homing endonucleases
View Download 0.238 0.886 pre-mRNA splicing factor activity d.15.6 Superantigen toxins, C-terminal domain
View Download 0.237 0.886 pre-mRNA splicing factor activity b.1.14 Invasin/intimin cell-adhesion fragments
View Download 0.234 0.886 pre-mRNA splicing factor activity c.52.2 tRNA splicing endonuclease, C-terminal domain
View Download 0.220 0.886 pre-mRNA splicing factor activity a.138.1 Multiheme cytochromes
View Download 0.219 0.886 pre-mRNA splicing factor activity b.60.1 Lipocalins

Predicted Domain #7
Region A:
Residues: [2152-2322]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 WRKSAIANTL LYLRLKNIYV SADDFVEEQN VYVLPKNLLK KFIEISDVKI QVAAFIYGMS  60
   61 AKDHPKVKEI KTVVLVPQLG HVGSVQISNI PDIGDLPDTE GLELLGWIHT QTEELKFMAA 120
  121 SEVATHSKLF ADKKRDCIDI SIFSTPGSVS LSAYNLTDEG YQWGEENKDI M

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #8
Region A:
Residues: [2323-2413]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NVLSEGFEPT FSTHAQLLLS DRITGNFIIP SGNVWNYTFM GTAFNQEGDY NFKYGIPLEF  60
   61 YNEMHRPVHF LQFSELAGDE ELEAEQIDVF S

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.546 d.19.1 MHC antigen-recognition domain
View Download 0.570 b.36.1 PDZ domain-like
View Download 0.510 d.93.1 SH2 domain
View Download 0.531 d.16.1 FAD-linked reductases, C-terminal domain
View Download 0.498 c.9.1 Barstar (barnase inhibitor)
View Download 0.449 d.50.2 Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain
View Download 0.442 d.129.1 TATA-box binding protein-like
View Download 0.441 b.71.1 Glycosyl hydrolase domain
View Download 0.425 b.61.4 Quinohemoprotein amine dehydrogenase A chain, domain 3
View Download 0.396 b.71.1 Glycosyl hydrolase domain
View Download 0.394 d.20.1 UBC-like
View Download 0.387 d.93.1 SH2 domain
View Download 0.383 d.50.1 dsRNA-binding domain-like
View Download 0.359 b.40.2 Bacterial enterotoxins
View Download 0.352 d.233.1 Inhibitor of vertebrate lysozyme, Ivy
View Download 0.350 d.129.2 Phosphoglucomutase, C-terminal domain
View Download 0.347 d.204.1 Ribosome binding protein Y (YfiA homologue)
View Download 0.332 d.50.1 dsRNA-binding domain-like
View Download 0.329 b.1.20 Tp47 lipoprotein, middle and C-terminal domains
View Download 0.323 b.1.18 E set domains
View Download 0.320 d.93.1 SH2 domain
View Download 0.287 b.71.1 Glycosyl hydrolase domain
View Download 0.284 b.1.2 Fibronectin type III
View Download 0.280 d.49.1 Signal recognition particle alu RNA binding heterodimer, SRP9/14
View Download 0.259 b.1.5 Transglutaminase, two C-terminal domains
View Download 0.243 d.87.2 CO dehydrogenase flavoprotein C-terminal domain-like
View Download 0.236 b.1.1 Immunoglobulin
View Download 0.226 d.21.1 Diaminopimelate epimerase-like
View Download 0.225 b.40.4 Nucleic acid-binding proteins
View Download 0.220 a.28.1 ACP-like
View Download 0.215 d.52.3 Prokaryotic type KH domain (KH-domain type II)
View Download 0.212 d.15.1 Ubiquitin-like
View Download 0.210 d.198.1 Type III secretory system chaperone
View Download 0.205 d.20.1 UBC-like
View Download 0.202 d.16.1 FAD-linked reductases, C-terminal domain

Predicted Domain #9
Region A:
Residues: [1331-1527]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VAAKWNTSLI SLFTYFREAI VATEPLLDIL VKGETRIQNR VKLGLNSKMP TRFPPAVFYT  60
   61 PKELGGLGMI SASHILIPAS DLSWSKQTDT GITHFRAGMT HEDEKLIPTI FRYITTWENE 120
  121 FLDSQRVWAE YATKRQEAIQ QNRRLAFEEL EGSWDRGIPR ISTLFQRDRH TLAYDRGHRI 180
  181 RREFKQYSLE RNSPFWW

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #10
Region A:
Residues: [1528-1578]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TNSHHDGKLW NLNAYRTDVI QALGGIETIL EHTLFKGTGF NSWEGLFWEK A

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #11
Region A:
Residues: [1579-1650]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SGFEDSMQFK KLTHAQRTGL SQIPNRRFTL WWSPTINRAN VYVGFLVQLD LTGIFLHGKI  60
   61 PTLKISLIQI FR

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.697 N/A N/A d.93.1 SH2 domain
View Download 0.667 N/A N/A a.1.1 Globin-like
View Download 0.644 N/A N/A a.1.1 Globin-like
View Download 0.604 N/A N/A d.130.1 S-adenosylmethionine synthetase
View Download 0.595 N/A N/A d.93.1 SH2 domain

Predicted Domain #12
Region A:
Residues: [1651-1743]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPRK SYKMNSSAAD ITMESVHEWE  60
   61 VSKPSLLHET NDSFKGLITN KMWFDVQLRY GDY

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #13
Region A:
Residues: [1744-1998]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DSHDISRYVR AKFLDYTTDN VSMYPSPTGV MIGIDLAYNM YDAYGNWFNG LKPLIQNSMR  60
   61 TIMKANPALY VLRERIRKGL QIYQSSVQEP FLNSSNYAEL FNNDIKLFVD DTNVYRVTVH 120
  121 KTFEGNVATK AINGCIFTLN PKTGHLFLKI IHTSVWAGQK RLSQLAKWKT AEEVSALVRS 180
  181 LPKEEQPKQI IVTRKAMLDP LEVHMLDFPN IAIRPTELRL PFSAAMSIDK LSDVVMKATE 240
  241 PQMVLFNIYD DWLDR

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #14
Region A:
Residues: [1999-2070]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ISSYTAFSRL TLLLRALKTN EESAKMILLS DPTITIKSYH LWPSFTDEQW ITIESQMRDL  60
   61 ILTEYGRKYN VN

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #15
Region A:
Residues: [2071-2413]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ISALTQTEIK DIILGQNIKA PSVKRQKMAE LEAARSEKQN DEEAAGASTV MKTKTINAQG  60
   61 EEIVVVASAD YESQTFSSKN EWRKSAIANT LLYLRLKNIY VSADDFVEEQ NVYVLPKNLL 120
  121 KKFIEISDVK IQVAAFIYGM SAKDHPKVKE IKTVVLVPQL GHVGSVQISN IPDIGDLPDT 180
  181 EGLELLGWIH TQTEELKFMA ASEVATHSKL FADKKRDCID ISIFSTPGSV SLSAYNLTDE 240
  241 GYQWGEENKD IMNVLSEGFE PTFSTHAQLL LSDRITGNFI IPSGNVWNYT FMGTAFNQEG 300
  301 DYNFKYGIPL EFYNEMHRPV HFLQFSELAG DEELEAEQID VFS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 93.69897
Match: 2og4A
Description: No description for 2og4A was found.

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle