YRC Public Data Repository - Protein Structure Prediction

Protein:	GUT1
Organism:	Saccharomyces cerevisiae
Length:	709 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GUT1.

Description	E-value	Query Range	Subject Range
GUT1 - Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is medi... GLPK_YEAST - Glycerol kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GUT1 PE=3 SV=1	0.0	[1..709]	[1..709]
GLPK2_THEMA, GLK... - Glycerol kinase 2 OS=Thermotoga maritima GN=glpK2 PE=3 SV=2, (Q9X1E4) Glycerol kinase 2 (EC 2.7.1.30... GLPK2_THEMA - Glycerol kinase 2 OS=Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) GN=glpK2 ...	0.0	[115..696]	[2..496]
gi\|7434247 - gi\|7434247\|pir\|\|C72254 glycerol kinase - Thermotoga maritima (strain MSB8) gi\|15644181, gi\|... - gi\|4981995\|gb\|AAD36500.1\|AE001795_3 glycerol kinase [Thermotoga maritima MSB8], gi\|15644181\|ref\|NP_2...	0.0	[115..682]	[2..482]
gi\|209752298, gi... - gi\|25287510\|pir\|\|C86082 glycerol kinase [imported] - Escherichia coli (strain O157:H7, substrain EDL... gi\|209914665, gi... - gi\|209921406\|ref\|YP_002295490.1\| glycerol kinase [Escherichia coli SE11], gi\|209914665\|dbj\|BAG79739.... gi\|15804515, gi\|... - gi\|15804515\|ref\|NP_290555.1\| glycerol kinase [Escherichia coli O157:H7 EDL933], gi\|12518827\|gb\|AAG59... gi\|115515317, gi... - gi\|117626193\|ref\|YP_859516.1\| glycerol kinase [Escherichia coli APEC O1], gi\|115515317\|gb\|ABJ03392.1...	0.0	[106..698]	[3..509]
GLPK_BACSU - Glycerol kinase OS=Bacillus subtilis GN=glpK PE=1 SV=1 gi\|2633252, gi\|1... - gi\|2633252\|emb\|CAB12757.1\| glycerol kinase [Bacillus subtilis subsp. subtilis str. 168], gi\|16077994...	0.0	[116..693]	[4..495]
gi\|144982459 - gi\|144982459\|gb\|EDJ90025.1\| glycerol kinase [Haemophilus influenzae R3021] gi\|145271495, gi... - gi\|145639448\|ref\|ZP_01795053.1\| glycerol kinase [Haemophilus influenzae PittII], gi\|145271495\|gb\|EDK... gi\|145637622, gi... - gi\|145637622\|ref\|ZP_01793278.1\| glycerol kinase [Haemophilus influenzae PittHH], gi\|145269219\|gb\|EDK... gi\|145631898 - gi\|145631898\|ref\|ZP_01787654.1\| glycerol kinase [Haemophilus influenzae 22.4-21] GLPK_HAEIN - Glycerol kinase OS=Haemophilus influenzae GN=glpK PE=3 SV=2 gi\|42630951 - gi\|42630951\|ref\|ZP_00156489.1\| COG0554: Glycerol kinase [Haemophilus influenzae R2866] GLPK_HAEIN - Glycerol kinase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) GN=glpK PE=3 S... gi\|229811540, gi... - gi\|229845524\|ref\|ZP_04465652.1\| glycerol kinase [Haemophilus influenzae 6P18H1], gi\|229811540\|gb\|EEP...	0.0	[111..698]	[1..502]
GLPK_CLOPE - Glycerol kinase OS=Clostridium perfringens GN=glpK PE=3 SV=1 GLPK_CLOPE - Glycerol kinase OS=Clostridium perfringens (strain 13 / Type A) GN=glpK PE=3 SV=1 gi\|168205814, gi... - gi\|170662693\|gb\|EDT15376.1\| glycerol kinase [Clostridium perfringens E str. JGS1987], gi\|168205814\|r... gi\|182624319, gi... - gi\|182624319\|ref\|ZP_02952104.1\| glycerol kinase [Clostridium perfringens D str. JGS1721], gi\|1779105...	0.0	[114..697]	[2..500]
gi\|191166449, gi... - gi\|75230447\|ref\|ZP_00716933.1\| COG0554: Glycerol kinase [Escherichia coli B7A], gi\|191166449\|ref\|ZP_... gi\|191172696, gi... - gi\|75240319\|ref\|ZP_00724259.1\| COG0554: Glycerol kinase [Escherichia coli F11], gi\|191172696\|ref\|ZP_... GLPK_ECOHS - Glycerol kinase OS=Escherichia coli O9:H4 (strain HS) GN=glpK PE=3 SV=1 gi\|194414525, gi... - gi\|75211260\|ref\|ZP_00711359.1\| COG0554: Glycerol kinase [Escherichia coli B171], gi\|194427485\|ref\|ZP... GLPK_SHIBS - Glycerol kinase OS=Shigella boydii serotype 4 (strain Sb227) GN=glpK PE=3 SV=1 gi\|124501289, gi... - gi\|124527587\|ref\|ZP_01699052.1\| glycerol kinase [Escherichia coli B], gi\|124501289\|gb\|EAY48754.1\| gl... gi\|89110103, gi\|... - gi\|89110103\|ref\|AP_003883.1\| glycerol kinase [Escherichia coli str. K-12 substr. W3110], gi\|85676134... GLPK_ECOLI - Glycerol kinase (EC 2.7.1.30) (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK) - Escherichia... gi\|75258091, gi\|... - gi\|75258091\|ref\|ZP_00729552.1\| COG0554: Glycerol kinase [Escherichia coli E22], gi\|193063920\|ref\|ZP_... GLPK_ECO57 - Glycerol kinase OS=Escherichia coli O157:H7 GN=glpK PE=3 SV=2 gi\|188493155, gi... - gi\|75513888\|ref\|ZP_00736241.1\| COG0554: Glycerol kinase [Escherichia coli 53638], gi\|188493155\|ref\|Z... EG10398 - glycerol kinase / Glycerol kinase EG10398 - glycerol kinase / Glycerol kinase gi\|83586230, gi\|... - gi\|83586230\|ref\|ZP_00924866.1\| COG0554: Glycerol kinase [Escherichia coli 101-1], gi\|194438285\|ref\|Z... GLPK_ECO24 - Glycerol kinase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=glpK PE=3 SV=1 gi\|83569808, gi\|... - gi\|83569808\|ref\|ZP_00921257.1\| COG0554: Glycerol kinase [Shigella dysenteriae 1012], gi\|194433205\|re... gi\|15834105, gi\|... - gi\|15834105\|ref\|NP_312878.1\| glycerol kinase [Escherichia coli O157:H7 str. Sakai], gi\|13364327\|dbj\|... GLPK_ECOL5 - Glycerol kinase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=glpK PE=3 SV=1 GLPK_ECOBW - Glycerol kinase OS=Escherichia coli (strain K12 / MC4100 / BW2952) GN=glpK PE=3 SV=1 gi\|251787189, gi... - gi\|251787189\|ref\|YP_003001493.1\| hypothetical protein B21_03760 [Escherichia coli BL21], gi\|24237946... gi\|189367919, gi... - gi\|189367919\|gb\|EDU86335.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4501], gi\|168761692\|ref... gi\|253326479, gi... - gi\|254290627\|ref\|YP_003056375.1\| glycerol kinase [Escherichia coli BL21(DE3)], gi\|253979934\|gb\|ACT45... gi\|188016761, gi... - gi\|188016761\|gb\|EDU54883.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4113], gi\|168748778\|ref... gi\|253975777, gi... - gi\|254163876\|ref\|YP_003046984.1\| glycerol kinase [Escherichia coli B str. REL606], gi\|253975777\|gb\|A... gi\|254795926, gi... - gi\|254795926\|ref\|YP_003080763.1\| glycerol kinase [Escherichia coli O157:H7 str. TW14359], gi\|2545953... gi\|208813468, gi... - gi\|208813468\|ref\|ZP_03254797.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4045], gi\|208734745... GLPK_ECOLC - Glycerol kinase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=glpK PE=3 SV=1 gi\|208729315, gi... - gi\|208809514\|ref\|ZP_03251851.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4206], gi\|208729315... GLPK_ECO5E - Glycerol kinase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=glpK PE=3 SV=1 gi\|208740827, gi... - gi\|208820704\|ref\|ZP_03261024.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4042], gi\|208740827... GLPK_ECO45 - Glycerol kinase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=glpK PE=3 SV=1 gi\|217324911, gi... - gi\|217324911\|ref\|ZP_03440995.1\| glycerol kinase [Escherichia coli O157:H7 str. TW14588], gi\|21732113... GLPK_ECOLU - Glycerol kinase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=glpK PE=3 SV=1 GLPK_ECO55 - Glycerol kinase OS=Escherichia coli (strain 55989 / EAEC) GN=glpK PE=3 SV=1 gi\|187769222, gi... - gi\|187769222\|gb\|EDU33066.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4196], gi\|168775240\|ref... gi\|168768227, gi... - gi\|189362669\|gb\|EDU81088.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4486], gi\|168768227\|ref... gi\|189373269, gi... - gi\|189373269\|gb\|EDU91685.1\| glycerol kinase [Escherichia coli O157:H7 str. EC869], gi\|168786781\|ref\|... gi\|189001421, gi... - gi\|189001421\|gb\|EDU70407.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4076], gi\|168780842\|ref... GLPK_ECODH - Glycerol kinase OS=Escherichia coli (strain K12 / DH10B) GN=glpK PE=3 SV=1 gi\|168799495, gi... - gi\|189378059\|gb\|EDU96475.1\| glycerol kinase [Escherichia coli O157:H7 str. EC508], gi\|168799495\|ref\|... gi\|195937568 - gi\|195937568\|ref\|ZP_03082950.1\| glycerol kinase [Escherichia coli O157:H7 str. EC4024] GLPK_ECOSM - Glycerol kinase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=glpK PE=3 SV=1	0.0	[111..698]	[1..502]
GLPK_PASMU - Glycerol kinase OS=Pasteurella multocida GN=glpK PE=3 SV=1 GLPK_PASMU - Glycerol kinase OS=Pasteurella multocida (strain Pm70) GN=glpK PE=3 SV=1	0.0	[111..698]	[1..502]
GLPK_CLOAB - Glycerol kinase OS=Clostridium acetobutylicum GN=glpK PE=3 SV=1 GLPK_CLOAB - Glycerol kinase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM...	0.0	[114..695]	[2..498]

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Predicted Domain #1

Region A:
Residues: [1-103]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFPSLFRLVV FSKRYIFRSS QRLYTSLKQE QSRMSKIMED LRSDYVPLIA SIDVGTTSSR  60
   61 CILFNRWGQD VSKHQIEYST SASKGKIGVS GLRRPSTAPA RET

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.560	c.47.1	Thioredoxin-like
View	Download	0.652	d.122.1	ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
View	Download	0.768	d.52.3	Prokaryotic type KH domain (KH-domain type II)
View	Download	0.601	d.233.1	Inhibitor of vertebrate lysozyme, Ivy
View	Download	0.560	d.130.1	S-adenosylmethionine synthetase
View	Download	0.470	d.102.1	Regulatory factor Nef
View	Download	0.462	b.61.3	D-aminopeptidase, middle and C-terminal domains
View	Download	0.462	a.74.1	Cyclin-like
View	Download	0.454	d.58.7	RNA-binding domain, RBD
View	Download	0.444	d.57.1	DNA damage-inducible protein DinI
View	Download	0.435	c.47.1	Thioredoxin-like
View	Download	0.427	a.133.1	Phospholipase A2, PLA2
View	Download	0.416	d.58.49	YajQ-like
View	Download	0.415	a.60.9	lambda integrase-like, N-terminal domain
View	Download	0.412	d.52.1	Alpha-lytic protease prodomain
View	Download	0.410	d.58.16	Poly(A) polymerase, C-terminal domain
View	Download	0.404	a.24.17	Group V grass pollen allergen
View	Download	0.396	d.38.1	Thioesterase/thiol ester dehydrase-isomerase
View	Download	0.396	b.61.1	Avidin/streptavidin
View	Download	0.395	a.2.11	Fe,Mn superoxide dismutase (SOD), N-terminal domain
View	Download	0.381	c.47.1	Thioredoxin-like
View	Download	0.380	a.4.9	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3
View	Download	0.376	d.17.2	Copper amine oxidase, domains 1 and 2
View	Download	0.372	d.131.1	DNA clamp
View	Download	0.363	d.204.1	Ribosome binding protein Y (YfiA homologue)
View	Download	0.353	a.24.10	Histidine-containing phosphotransfer domain, HPT domain
View	Download	0.352	d.17.4	NTF2-like
View	Download	0.345	d.81.1	Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
View	Download	0.333	a.45.1	Glutathione S-transferase (GST), C-terminal domain
View	Download	0.331	a.24.17	Group V grass pollen allergen
View	Download	0.331	d.58.4	Dimeric alpha+beta barrel
View	Download	0.299	c.55.4	Translational machinery components
View	Download	0.298	d.49.1	Signal recognition particle alu RNA binding heterodimer, SRP9/14
View	Download	0.297	d.58.7	RNA-binding domain, RBD
View	Download	0.296	d.44.1	Fe,Mn superoxide dismutase (SOD), C-terminal domain
View	Download	0.295	d.65.1	Hedgehog/DD-pepidase
View	Download	0.293	a.121.1	Tetracyclin repressor-like, C-terminal domain
View	Download	0.290	d.44.1	Fe,Mn superoxide dismutase (SOD), C-terminal domain
View	Download	0.277	d.17.2	Copper amine oxidase, domains 1 and 2
View	Download	0.271	a.2.11	Fe,Mn superoxide dismutase (SOD), N-terminal domain
View	Download	0.267	d.212.1	TolA/TonB C-terminal domain
View	Download	0.265	a.25.1	Ferritin-like
View	Download	0.257	d.50.1	dsRNA-binding domain-like
View	Download	0.256	d.58.12	eEF-1beta-like
View	Download	0.251	d.88.1	SRF-like
View	Download	0.243	a.7.8	GAT domain
View	Download	0.238	d.19.1	MHC antigen-recognition domain
View	Download	0.237	a.13.1	alpha-2-Macroglobulin receptor associated protein (RAP) domain 1
View	Download	0.237	c.48.1	TK C-terminal domain-like
View	Download	0.237	a.80.1	DNA polymerase III clamp loader subunits, C-terminal domain
View	Download	0.234	d.58.1	4Fe-4S ferredoxins
View	Download	0.234	c.51.1	Anticodon-binding domain of Class II aaRS
View	Download	0.227	c.58.1	Aminoacid dehydrogenase-like, N-terminal domain
View	Download	0.227	f.36.1	Neurotransmitter-gated ion-channel pransmembrane pore
View	Download	0.218	d.58.21	Molybdenum cofactor biosynthesis protein C, MoaC
View	Download	0.217	d.49.1	Signal recognition particle alu RNA binding heterodimer, SRP9/14
View	Download	0.217	a.24.9	alpha-catenin/vinculin
View	Download	0.216	a.26.1	4-helical cytokines
View	Download	0.214	a.60.6	DNA polymerase beta, N-terminal domain-like
View	Download	0.212	d.58.7	RNA-binding domain, RBD
View	Download	0.210	a.22.1	Histone-fold
View	Download	0.208	d.32.1	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase
View	Download	0.208	a.100.1	6-phosphogluconate dehydrogenase C-terminal domain-like
View	Download	0.203	d.208.1	MTH1598-like
View	Download	0.203	d.41.5	Molybdopterin synthase subunit MoaE

Predicted Domain #2

Region A:
Residues: [104-384]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PNAGDIKTSG KPIFSAEGYA IQETKFLKIE ELDLDFHNEP TLKFPKPGWV ECHPQKLLVN  60
   61 VVQCLASSLL SLQTINSERV ANGLPPYKVI CMGIANMRET TILWSRRTGK PIVNYGIVWN 120
  121 DTRTIKIVRD KWQNTSVDRQ LQLRQKTGLP LLSTYFSCSK LRWFLDNEPL CTKAYEENDL 180
  181 MFGTVDTWLI YQLTKQKAFV SDVTNASRTG FMNLSTLKYD NELLEFWGID KNLIHMPEIV 240
  241 SSSQYYGDFG IPDWIMEKLH DSPKTVLRDL VKRNLPIQGC L

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	996.54902
Match:	1bo5O_
Description:	Glycerol kinase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glycerol kinase activity	5.25911418019759	bayes_pls_golite062009
carbohydrate kinase activity	2.33338276303563	bayes_pls_golite062009
hexokinase activity	1.70516840340504	bayes_pls_golite062009
glucose binding	1.58717365638543	bayes_pls_golite062009
binding	1.44744777288539	bayes_pls_golite062009
protein binding	0.704303457266874	bayes_pls_golite062009
glucokinase activity	0.47468456642723	bayes_pls_golite062009
sugar binding	0.44352406324938	bayes_pls_golite062009
kinase activity	0.345540463799773	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.324655711623803	bayes_pls_golite062009
catalytic activity	0.296484721581333	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.214617425075414	bayes_pls_golite062009
monosaccharide binding	0.20435276425198	bayes_pls_golite062009
nucleic acid binding	0.132883501060045	bayes_pls_golite062009
hydrolase activity	0.13181840194556	bayes_pls_golite062009
transferase activity	0.105619024604158	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [385-457]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GDQSASMVGQ LAYKPGAAKC TYGTGCFLLY NTGTKKLISQ HGALTTLAFW FPHLQEYGGQ  60
   61 KPELSKPHFA LEG

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [611-645]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AECTALGAAI AANMAFKDVN ERPLWKDLHD VKKWV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	996.54902
Match:	1bo5O_
Description:	Glycerol kinase
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [458-610]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SVAVAGAVVQ WLRDNLRLID KSEDVGPIAS TVPDSGGVVF VPAFSGLFAP YWDPDARATI  60
   61 MGMSQFTTAS HIARAAVEGV CFQARAILKA MSSDAFGEGS KDRDFLEEIS DVTYEKSPLS 120
  121 VLAVDGGMSR SNEVMQIQAD ILGPCVKVRR SPT

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [646-709]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FYNGMEKNEQ ISPEAHPNLK IFRSESDDAE RRKHWKYWEV AVERSKGWLK DIEGEHEQVL  60
   61 ENFQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	996.54902
Match:	1bo5O_
Description:	Glycerol kinase
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: