YRC Public Data Repository - Protein Structure Prediction

Protein:	MBHM_ECOLI
Organism:	Escherichia coli
Length:	567 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MBHM_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|110643234, gi... - gi\|110643234\|ref\|YP_670964.1\| hydrogenase 2 large subunit [Escherichia coli 536], gi\|110344826\|gb\|AB...	571.0	[0..1]	[567..1]
gi\|81242385, gi\|... - gi\|82778237\|ref\|YP_404586.1\| hydrogenase 2 large subunit [Shigella dysenteriae Sd197], gi\|81242385\|g...	571.0	[0..1]	[567..1]
gi\|218428849, gi... - gi\|218691285\|ref\|YP_002399497.1\| hydrogenase 2 large subunit [Escherichia coli ED1a], gi\|218428849\|e... gi\|227887701, gi... - gi\|227887701\|ref\|ZP_04005506.1\| hydrogenase (acceptor) [Escherichia coli 83972], gi\|227835097\|gb\|EEJ... gi\|75240550, gi\|... - gi\|75240550\|ref\|ZP_00724478.1\| COG0374: Ni,Fe-hydrogenase I large subunit [Escherichia coli F11], gi...	571.0	[0..1]	[567..1]
gi\|110616445, gi... - gi\|110806897\|ref\|YP_690417.1\| hydrogenase 2 large subunit [Shigella flexneri 5 str. 8401], gi\|110616...	569.0	[0..1]	[567..1]
gi\|26249562, gi\|... - gi\|26249562\|ref\|NP_755602.1\| hydrogenase 2 large subunit [Escherichia coli CFT073], gi\|26109970\|gb\|A...	569.0	[0..1]	[567..1]
MBHL_BRADU - Uptake hydrogenase large subunit OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NB... MBHL_BRAJA - Uptake hydrogenase large subunit OS=Bradyrhizobium japonicum GN=hupB PE=3 SV=1	567.0	[0..2]	[566..15]
gi\|119354150, gi... - gi\|67934969\|ref\|ZP_00527994.1\| Nickel-dependent hydrogenase, large subunit [Chlorobium phaeobacteroi...	563.0	[0..1]	[567..1]
gi\|77701268, gi\|... - gi\|77738800\|ref\|ZP_00807293.1\| IMP dehydrogenase/GMP reductase:Nickel-dependent hydrogenase, large s...	559.0	[0..2]	[566..16]
gi\|206710209, gi... - gi\|207858406\|ref\|YP_002245057.1\| hydrogenase 2 large subunit [Salmonella enterica subsp. enterica se... gi\|199605021, gi... - gi\|200387923\|ref\|ZP_03214535.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica se... gi\|16421701, gi\|... - gi\|16766447\|ref\|NP_462062.1\| hydrogenase 2 large subunit [Salmonella typhimurium LT2], gi\|16421701\|g... gi\|205273846, gi... - gi\|205354065\|ref\|YP_002227866.1\| hydrogenase 2 large subunit [Salmonella enterica subsp. enterica se... gi\|197242930, gi... - gi\|197264675\|ref\|ZP_03164749.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica se... gi\|197939159, gi... - gi\|198244643\|ref\|YP_002217124.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica s... gi\|194401664, gi... - gi\|194443001\|ref\|YP_002042411.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica s... gi\|194448931, gi... - gi\|194448931\|ref\|YP_002047142.1\| hydrogenase 2 large subunit [Salmonella enterica subsp. enterica se... gi\|205348467, gi... - gi\|205348467\|gb\|EDZ35098.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica serova... lcl\|NC_016856.1_... - [gene=hybC] [protein=hydrogenase 2 large subunit] [protein_id=YP_005239310.1] [location=complement(3... gi\|168819798, gi... - gi\|205343401\|gb\|EDZ30165.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica serova... gi\|205331255, gi... - gi\|205331255\|gb\|EDZ18019.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica serova... gi\|205337811, gi... - gi\|205337811\|gb\|EDZ24575.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica serova... gi\|161365491, gi... - gi\|161616127\|ref\|YP_001590092.1\| hydrogenase 2 large subunit [Salmonella enterica subsp. enterica se... gi\|205323322, gi... - gi\|205323322\|gb\|EDZ11161.1\| hydrogenase-2, large subunit [Salmonella enterica subsp. enterica serova... gi\|62129291, gi\|... - gi\|62181658\|ref\|YP_218075.1\| hydrogenase 2 large subunit [Salmonella enterica subsp. enterica serova...	559.0	[0..1]	[567..1]
hupL - hupL hydrogenase large chain 1065304:1067097 forward MW:65672	559.0	[0..2]	[566..16]

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Predicted Domain #1

Region A:
Residues: [1-567]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI  60
   61 CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT 120
  121 SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP 180
  181 PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL 240
  241 MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP 300
  301 GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG 360
  361 KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS 420
  421 TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG 480
  481 MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI 540
  541 HSFDPCMACA VHVVDADGNE VVSVKVL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	143.0
Match:	1h2aL
Description:	Nickel-iron hydrogenase, large subunit
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity, acting on hydrogen as donor	6.92862518539575	bayes_pls_golite062009
ferredoxin hydrogenase activity	3.63852106070173	bayes_pls_golite062009
oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor	3.63852106070173	bayes_pls_golite062009
NADH dehydrogenase activity	3.2672032605265	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	2.70075876201392	bayes_pls_golite062009
NADH dehydrogenase (ubiquinone) activity	2.4952454159327	bayes_pls_golite062009
oxidoreductase activity, acting on iron-sulfur proteins as donors	2.45015987642707	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	2.19558031681108	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	2.05566803830683	bayes_pls_golite062009
oxidoreductase activity	1.80355547790706	bayes_pls_golite062009
catalytic activity	1.13542026154948	bayes_pls_golite062009
binding	0.33259738701549	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: